PMID- 34522782
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210916
IS  - 2391-5412 (Electronic)
IS  - 2391-5412 (Linking)
VI  - 16
IP  - 1
DP  - 2021
TI  - Clonal diversity of the B cell receptor repertoire in patients with coronary 
      in-stent restenosis and type 2 diabetes.
PG  - 884-898
LID - 10.1515/biol-2021-0091 [doi]
AB  - Type 2 diabetes mellitus (T2DM) is known as a risk factor for coronary in-stent 
      restenosis (ISR) in patients with coronary artery disease (CAD). Evidence 
      suggests that B cells play a functional role in the progression of 
      atherosclerotic lesions. However, the B cell receptor (BCR) repertoire in 
      patients with ISR remains unclear. This study aims to profile the BCR repertoire 
      in patients with coronary ISR/T2DM. A total of 21 CAD patients with or without 
      ISR/T2DM were enrolled. PBMCs were isolated and examined for BCR repertoire 
      profiles using DNA-seq. Our results showed that the diversity of amino acid 
      sequences in ISR DM patients was higher than that in ISR -DM patients. The 
      frequencies of 21 V/J paired genes differed between ISR DM and -ISR DM patients, 
      while frequencies of 5 V/J paired genes differed between ISR DM and ISR -DM. The 
      -ISR -DM group presented the highest clonotype overlap rate, while ISR DM 
      patients presented the lowest overlap rate. Our study presented the BCR 
      repertoires in patients with ISR/T2DM. The data suggested different BCR 
      signatures between patients with ISR and T2DM. Further analysis of BCR profiles 
      would enhance understanding of ISR.
CI  - (c) 2021 Ruiqiang Weng et al., published by De Gruyter.
FAU - Weng, Ruiqiang
AU  - Weng R
AD  - Research Experimental Center, Meizhou People's Hospital (Huangtang Hospital), 
      Meizhou Hospital Affiliated to Sun Yat-Sen University, Meizhou 514031, People's 
      Republic of China.
AD  - Guangdong Provincial Engineering and Technological Research Center for Molecular 
      Diagnostics of Cardiovascular Diseases, Meizhou 514031, People's Republic of 
      China.
AD  - Provincial Key Laboratory of Precision Medicine and Clinical Translational 
      Research of Hakka Population, Meizhou 514031, People's Republic of China.
AD  - Center for Precision Medicine, Meizhou People's Hospital (Huangtang Hospital), 
      Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, People's 
      Republic of China.
FAU - Liu, Sudong
AU  - Liu S
AD  - Research Experimental Center, Meizhou People's Hospital (Huangtang Hospital), 
      Meizhou Hospital Affiliated to Sun Yat-Sen University, Meizhou 514031, People's 
      Republic of China.
AD  - Guangdong Provincial Engineering and Technological Research Center for Molecular 
      Diagnostics of Cardiovascular Diseases, Meizhou 514031, People's Republic of 
      China.
AD  - Provincial Key Laboratory of Precision Medicine and Clinical Translational 
      Research of Hakka Population, Meizhou 514031, People's Republic of China.
AD  - Center for Precision Medicine, Meizhou People's Hospital (Huangtang Hospital), 
      Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, People's 
      Republic of China.
FAU - Gu, Xiaodong
AU  - Gu X
AD  - Research Experimental Center, Meizhou People's Hospital (Huangtang Hospital), 
      Meizhou Hospital Affiliated to Sun Yat-Sen University, Meizhou 514031, People's 
      Republic of China.
AD  - Guangdong Provincial Engineering and Technological Research Center for Molecular 
      Diagnostics of Cardiovascular Diseases, Meizhou 514031, People's Republic of 
      China.
AD  - Provincial Key Laboratory of Precision Medicine and Clinical Translational 
      Research of Hakka Population, Meizhou 514031, People's Republic of China.
AD  - Center for Precision Medicine, Meizhou People's Hospital (Huangtang Hospital), 
      Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, People's 
      Republic of China.
FAU - Zhong, Zhixiong
AU  - Zhong Z
AD  - Guangdong Provincial Engineering and Technological Research Center for Molecular 
      Diagnostics of Cardiovascular Diseases, Meizhou 514031, People's Republic of 
      China.
AD  - Provincial Key Laboratory of Precision Medicine and Clinical Translational 
      Research of Hakka Population, Meizhou 514031, People's Republic of China.
AD  - Center for Precision Medicine, Meizhou People's Hospital (Huangtang Hospital), 
      Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, People's 
      Republic of China.
AD  - Center for Cardiovascular Diseases, Meizhou People's Hospital (Huangtang 
      Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University, Meizhou 514031, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210828
PL  - Poland
TA  - Open Life Sci
JT  - Open life sciences
JID - 101669614
PMC - PMC8402935
OTO - NOTNLM
OT  - B cell receptor repertoire
OT  - in-stent restenosis
OT  - repertoire sequencing
OT  - type 2 diabetes mellitus
COIS- Conflict of interest: The authors state no conflict of interest.
EDAT- 2021/09/16 06:00
MHDA- 2021/09/16 06:01
PMCR- 2021/08/28
CRDT- 2021/09/15 07:20
PHST- 2020/09/09 00:00 [received]
PHST- 2021/05/23 00:00 [revised]
PHST- 2021/07/20 00:00 [accepted]
PHST- 2021/09/15 07:20 [entrez]
PHST- 2021/09/16 06:00 [pubmed]
PHST- 2021/09/16 06:01 [medline]
PHST- 2021/08/28 00:00 [pmc-release]
AID - biol-2021-0091 [pii]
AID - 10.1515/biol-2021-0091 [doi]
PST - epublish
SO  - Open Life Sci. 2021 Aug 28;16(1):884-898. doi: 10.1515/biol-2021-0091. 
      eCollection 2021.