PMID- 34528803
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20240403
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Print)
IS  - 1944-8244 (Linking)
VI  - 13
IP  - 38
DP  - 2021 Sep 29
TI  - Sorption of Neuropsychopharmaca in Microfluidic Materials for In Vitro Studies.
PG  - 45161-45174
LID - 10.1021/acsami.1c07639 [doi]
AB  - Sorption (i.e., adsorption and absorption) of small-molecule compounds to 
      polydimethylsiloxane (PDMS) is a widely acknowledged phenomenon. However, studies 
      to date have largely been conducted under atypical conditions for microfluidic 
      applications (lack of perfusion, lack of biological fluids, etc.), especially 
      considering biological studies such as organs-on-chips where small-molecule 
      sorption poses the largest concern. Here, we present an in-depth study of 
      small-molecule sorption under relevant conditions for microphysiological systems, 
      focusing on a standard geometry for biological barrier studies that find 
      application in pharmacokinetics. We specifically assess the sorption of a broad 
      compound panel including 15 neuropsychopharmaca at in vivo concentration levels. 
      We consider devices constructed from PDMS as well as two material alternatives 
      (off-stoichiometry thiol-ene-epoxy, or tape/polycarbonate laminates). Moreover, 
      we study the much neglected impact of peristaltic pump tubing, an essential 
      component of the recirculating systems required to achieve in vivo-like perfusion 
      shear stresses. We find that the choice of the device material does not have a 
      significant impact on the sorption behavior in our barrier-on-chip-type system. 
      Our PDMS observations in particular suggest that excessive compound sorption 
      observed in prior studies is not sufficiently described by compound 
      hydrophobicity or other suggested predictors. Critically, we show that sorption 
      by peristaltic tubing, including the commonly utilized PharMed BPT, dominates 
      over device sorption even on an area-normalized basis, let alone at the typically 
      much larger tubing surface areas. Our findings highlight the importance of 
      validating compound dosages in organ-on-chip studies, as well as the need for 
      considering tubing materials with equal or higher care than device materials.
FAU - Winkler, Thomas E
AU  - Winkler TE
AUID- ORCID: 0000-0002-2331-4833
AD  - Division of Micro- and Nanosystems, KTH Royal Institute of Technology, 10044 
      Stockholm, Sweden.
FAU - Herland, Anna
AU  - Herland A
AUID- ORCID: 0000-0002-5002-2537
AD  - Division of Micro- and Nanosystems, KTH Royal Institute of Technology, 10044 
      Stockholm, Sweden.
AD  - AIMES, Center for Integrated Medical and Engineering Science, Department of 
      Neuroscience, Department of Neuroscience, Karolinska Institute, Solna 17165, 
      Sweden.
LA  - eng
PT  - Journal Article
DEP - 20210916
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Central Nervous System Agents)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Fluorescent Dyes)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Adsorption
MH  - Central Nervous System Agents/chemistry/*isolation & purification
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Fluorescent Dyes/chemistry/isolation & purification
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Lab-On-A-Chip Devices
MH  - Microfluidic Analytical Techniques/instrumentation
PMC - PMC8485331
OTO - NOTNLM
OT  - materials
OT  - microfluidics
OT  - neuropsychopharmaca
OT  - non-specific binding
OT  - organs-on-chips
COIS- The authors declare no competing financial interest.
EDAT- 2021/09/17 06:00
MHDA- 2022/01/28 06:00
PMCR- 2021/10/01
CRDT- 2021/09/16 12:14
PHST- 2021/09/17 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
PHST- 2021/09/16 12:14 [entrez]
PHST- 2021/10/01 00:00 [pmc-release]
AID - 10.1021/acsami.1c07639 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2021 Sep 29;13(38):45161-45174. doi: 
      10.1021/acsami.1c07639. Epub 2021 Sep 16.