PMID- 34529151
OWN - NLM
STAT- MEDLINE
DCOM- 20211015
LR  - 20211015
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 166
IP  - 11
DP  - 2021 Nov
TI  - A strain of highly pathogenic porcine reproductive and respiratory syndrome 
      virus: genomic characterization, pathogenicity, and construction of an infectious 
      full-length cDNA clone.
PG  - 3127-3141
LID - 10.1007/s00705-021-05212-w [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is a highly contagious 
      infectious disease caused by porcine reproductive and respiratory syndrome virus 
      (PRRSV), which inflicts major economic losses on the global pig farming industry. 
      Based on its similarity to highly pathogenic strains, the GDzj strain isolated in 
      this study was predicted to be highly pathogenic. We therefore analyzed the 
      pathogenicity of this strain experimentally in piglets. All piglets challenged 
      with this virus experienced fever or high fever, loss of appetite, decreased food 
      intake, daily weight loss, shortness of breath, and listlessness, and the 
      necropsy results showed that they had experienced severe interstitial pneumonia. 
      We then used the BAC system to construct a full-length cDNA infectious clone of 
      GDzj, and the rescued virus displayed in vitro proliferation characteristics 
      similar to those of the parental PRRSV strain. In summary, we successfully 
      isolated a highly pathogenic PRRSV strain and constructed a full-length 
      infectious cDNA clone from it, thereby providing an effective reverse genetics 
      platform for further study of viral pathogenesis.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, 
      part of Springer Nature.
FAU - Wang, Shuangyun
AU  - Wang S
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China.
FAU - Liu, Yanling
AU  - Liu Y
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China.
FAU - Yu, Linyang
AU  - Yu L
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China.
FAU - Liang, Tairun
AU  - Liang T
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China.
FAU - Zhang, Pengfei
AU  - Zhang P
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China.
FAU - Dong, Jianguo
AU  - Dong J
AD  - School of Animal Husbandry and Medical Engineering, Xinyang Agriculture and 
      Forestry University, Xinyang, 464000, China.
FAU - Zhang, Leyi
AU  - Zhang L
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China.
FAU - Liang, Pengshuai
AU  - Liang P
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China.
FAU - Wang, Lei
AU  - Wang L
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China.
FAU - Xu, Zheng
AU  - Xu Z
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China. 
      26103686@qq.com.
FAU - Song, Changxu
AU  - Song C
AUID- ORCID: 0000-0001-5311-0439
AD  - College of Animal Science & National Engineering Center for Swine Breeding 
      Industry, South China Agriculture University, Guangzhou, 510642, China. 
      cxsong2004@163.com.
LA  - eng
GR  - 2018YFD0501100/the National Key Research and Development Program of China/
GR  - 201817SY0002/Major animal disease prevention and control technology research and 
      promotion projects/
GR  - 2019B020211003/Guangdong Province Key Field R&D Program Project/
GR  - ZDXK201702/the 2020 Youth Backbone Training Plan of Henan University of Higher 
      Education/
PT  - Journal Article
DEP - 20210916
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (DNA, Complementary)
SB  - IM
MH  - Animals
MH  - Chromosomes, Artificial, Bacterial
MH  - DNA, Complementary/genetics
MH  - Genome, Viral
MH  - Lung/virology
MH  - Lymph Nodes/pathology/virology
MH  - Phylogeny
MH  - Pneumonia, Viral/pathology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*etiology/virology
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/growth & 
      development/isolation & purification/*pathogenicity
MH  - Swine
EDAT- 2021/09/17 06:00
MHDA- 2021/10/16 06:00
CRDT- 2021/09/16 12:27
PHST- 2020/12/02 00:00 [received]
PHST- 2021/07/08 00:00 [accepted]
PHST- 2021/09/17 06:00 [pubmed]
PHST- 2021/10/16 06:00 [medline]
PHST- 2021/09/16 12:27 [entrez]
AID - 10.1007/s00705-021-05212-w [pii]
AID - 10.1007/s00705-021-05212-w [doi]
PST - ppublish
SO  - Arch Virol. 2021 Nov;166(11):3127-3141. doi: 10.1007/s00705-021-05212-w. Epub 
      2021 Sep 16.