PMID- 34532386
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220828
IS  - 2305-5839 (Print)
IS  - 2305-5847 (Electronic)
IS  - 2305-5839 (Linking)
VI  - 9
IP  - 15
DP  - 2021 Aug
TI  - LXA4 protects against blue-light induced retinal degeneration in human A2E-laden 
      RPE cells and Balb-c mice.
PG  - 1249
LID - 10.21037/atm-21-3390 [doi]
LID - 1249
AB  - BACKGROUND: Age-related macular degeneration (AMD) is one of the leading causes 
      of permanent visual impairment in the elderly. Blue light (BL) has been reported 
      to cause retinal damage and contribute to the onset and development of severe 
      AMD. N-retinylidene-N-retinylethanolamine (A2E), a lipofuscin fluorophore, 
      accumulates with ageing in the retinal pigment epithelium (RPE) cells. Once 
      exposed to BL, A2E easily oxidizes to A2E-epoxides, causing oxidative-stress 
      injury to the retina. Lipoxin A4 (LXA4), an endogenous anti-antioxidant lipid, 
      plays a key role in multiple organs by binding to the formyl-peptide 
      receptor-like 1 (FPRL1). This study examined the protective effects of LXA4 on 
      oxidative-stress injury induced by BL exposure, and clarified the underlying 
      mechanisms in cultured RPE cells and Balb-c mice. METHODS: LXA4 diluent was 
      orally administered to mice before retinal degeneration was established. Optical 
      coherence tomography, retinal histology, and RPE cell injury were assessed. 
      RESULTS: LXA4 administration significantly ameliorated retinal damage as 
      evidenced by the thicknesses of the retinal layers and the tight junctions of RPE 
      cells in vivo. LXA4 inhibited BL-induced reactive oxygen species (ROS) 
      production, reduced tight junctions, and the death of A2E-laden RPE cells. LXA4 
      also potently increased the expression of haem oxygenase-1 (HO1) and NAD(P)H 
      quinone oxidoreductase 1 (NQO1), probably by decreasing the association between 
      nuclear factor erythroid 2-related factor 2 (NRF2) and Kelch-like ECH 
      (Epichlorohydrin) -associated protein 1 (Keap1), and ameliorating NRF2 nuclear 
      translocation and the antioxidant response element (ARE) deoxyribonucleic acid 
      (DNA) binding activity. CONCLUSIONS: Our results showed that LXA4 ameliorated 
      retinal degeneration, and should be considered in the prevention and treatment of 
      AMD.
CI  - 2021 Annals of Translational Medicine. All rights reserved.
FAU - Xie, Tianhua
AU  - Xie T
AD  - Department of Ophthalmology, Shanghai General Hospital of Nanjing Medical 
      University, Shanghai, China.
AD  - Department of Ophthalmology, The Affiliated Wuxi People's Hospital of Nanjing 
      Medical University, Wuxi, China.
FAU - Cai, Jiping
AU  - Cai J
AD  - Department of Ophthalmology, The Affiliated Wuxi People's Hospital of Nanjing 
      Medical University, Wuxi, China.
FAU - Yao, Yong
AU  - Yao Y
AD  - Department of Ophthalmology, The Affiliated Wuxi People's Hospital of Nanjing 
      Medical University, Wuxi, China.
FAU - Sun, Chao
AU  - Sun C
AD  - Department of Ophthalmology, The Affiliated Wuxi People's Hospital of Nanjing 
      Medical University, Wuxi, China.
FAU - Yang, Qian
AU  - Yang Q
AD  - Department of Ophthalmology, The Affiliated Wuxi People's Hospital of Nanjing 
      Medical University, Wuxi, China.
FAU - Wu, Meili
AU  - Wu M
AD  - Center of Clinical Research, The Affiliated Wuxi People's Hospital of Nanjing 
      Medical University, Wuxi, China.
FAU - Xu, Zifan
AU  - Xu Z
AD  - Department of Ophthalmology, The Affiliated Wuxi People's Hospital of Nanjing 
      Medical University, Wuxi, China.
FAU - Sun, Xiaodong
AU  - Sun X
AD  - Department of Ophthalmology, Shanghai General Hospital of Nanjing Medical 
      University, Shanghai, China.
FAU - Wang, Xiaolu
AU  - Wang X
AD  - Center of Clinical Research, The Affiliated Wuxi People's Hospital of Nanjing 
      Medical University, Wuxi, China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Ann Transl Med
JT  - Annals of translational medicine
JID - 101617978
PMC - PMC8421929
OTO - NOTNLM
OT  - Age-related macular degeneration (AMD)
OT  - blue light
OT  - lipoxin A4 (LXA4)
OT  - oxidative stress
OT  - retinal degeneration
COIS- Conflicts of Interest: All authors have completed the ICMJE uniform disclosure 
      form (available at https://dx.doi.org/10.21037/atm-21-3390). The authors have no 
      conflicts of interest to declare.
EDAT- 2021/09/18 06:00
MHDA- 2021/09/18 06:01
PMCR- 2021/08/01
CRDT- 2021/09/17 07:19
PHST- 2021/06/08 00:00 [received]
PHST- 2021/08/05 00:00 [accepted]
PHST- 2021/09/17 07:19 [entrez]
PHST- 2021/09/18 06:00 [pubmed]
PHST- 2021/09/18 06:01 [medline]
PHST- 2021/08/01 00:00 [pmc-release]
AID - atm-09-15-1249 [pii]
AID - 10.21037/atm-21-3390 [doi]
PST - ppublish
SO  - Ann Transl Med. 2021 Aug;9(15):1249. doi: 10.21037/atm-21-3390.