PMID- 34535266
OWN - NLM
STAT- MEDLINE
DCOM- 20210921
LR  - 20210921
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 1179
DP  - 2021 Sep 22
TI  - Non-invasive and accurate readout of superoxide anion in biological systems by 
      near-infrared light.
PG  - 338827
LID - S0003-2670(21)00653-X [pii]
LID - 10.1016/j.aca.2021.338827 [doi]
AB  - Infectious and inflammatory diseases involve superoxide anion (O(2)(*-)) 
      production. Real-time and non-invasive evaluation of O(2)(*-) in intact 
      biological systems has been a significant challenge in biology and medicine. 
      Here, I report that an advanced near-infrared chemiluminescent probe, MCLA-800, 
      enables reliable non-invasive optical readout of O(2)(*-)ex vivo and in vivo. 
      MCLA-800 allowed highly selective and sensitive monitoring of O(2)(*-) in 
      undiluted human whole blood ex vivo. For the first time, the use of MCLA-800 
      revealed two reproducible types of O(2)(*-) production in response to stimulation 
      by unopsonized zymosan particles of Saccharomyces cerevisiae, that is, slow 
      response (S-type) and fast response (F-type), specific to each individual. 
      O(2)(*-) production was synchronized with myeloperoxidase (MPO) activation in the 
      former type but not in the latter. Moreover, as new findings, MCLA-800 
      chemiluminescence demonstrated that the chemiluminescence intensity-time 
      properties of formyl-methionyl-leucyl-phenylalanine (fMLP)- or phorbol 
      12-myristate 13-acetate (PMA)-induced O(2)(*-) production and MPO activity were 
      independent of S- and F-type zymosan-induced MCLA-800 chemiluminescence whole 
      blood and that PMA-induced MPO activation synchronized with PMA-induced O(2)(*-) 
      production in S- and F-type zymosan-induced MCLA-800 chemiluminescence whole 
      blood, but fMLP-induced MPO activation did not synchronize with fMLP-induced 
      O(2)(*-) production in both of S- and F-type blood. Furthermore, MCLA-800 
      spatiotemporally allowed non-invasive and clear in vivo imaging of O(2)(*-) in 
      animal models of acute dermatitis and focal arthritis. Therefore, MCLA-800 could 
      be possibly applied in various advanced diagnostic techniques.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Teranishi, Katsunori
AU  - Teranishi K
AD  - Graduate School of Bioresources, Mie University, 1577 Kurimamachiya, Tsu, Mie, 
      514-8507, Japan. Electronic address: teranisi@bio.mie-u.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20210705
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 11062-77-4 (Superoxides)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Luminescent Measurements
MH  - N-Formylmethionine Leucyl-Phenylalanine
MH  - Peroxidase
MH  - *Superoxides
MH  - Tetradecanoylphorbol Acetate
OTO - NOTNLM
OT  - Bioimaging
OT  - Chemiluminescence
OT  - Imaging agent
OT  - Near-infrared luminescence
OT  - Superoxide anion
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/09/19 06:00
MHDA- 2021/09/22 06:00
CRDT- 2021/09/18 05:31
PHST- 2021/04/30 00:00 [received]
PHST- 2021/06/21 00:00 [revised]
PHST- 2021/07/02 00:00 [accepted]
PHST- 2021/09/18 05:31 [entrez]
PHST- 2021/09/19 06:00 [pubmed]
PHST- 2021/09/22 06:00 [medline]
AID - S0003-2670(21)00653-X [pii]
AID - 10.1016/j.aca.2021.338827 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2021 Sep 22;1179:338827. doi: 10.1016/j.aca.2021.338827. Epub 
      2021 Jul 5.