PMID- 34539009
OWN - NLM
STAT- MEDLINE
DCOM- 20211026
LR  - 20231213
IS  - 1735-3688 (Electronic)
IS  - 0253-0716 (Print)
IS  - 0253-0716 (Linking)
VI  - 46
IP  - 5
DP  - 2021 Sep
TI  - The Relationship between the Connexin 32 and Connexin 43 Genes and the 
      Pretreatment Stage and Short-term Follow-up of Patients with Acute Myeloid 
      Leukemia.
PG  - 347-354
LID - 10.30476/ijms.2020.84511.1477 [doi]
AB  - BACKGROUND: Connexins (Cxs) are gap junction proteins involved in the 
      communication between acute myeloid leukemia (AML) and stromal cells. They 
      consist of intercellular channels termed "connexions", which can cause 
      uncontrolled cell proliferation if dysregulated. This study aimed to evaluate the 
      expression levels of the Cx32 and Cx43 genes and their correlations with other 
      prognostic markers in patients with AML. METHODS: This cross sectional study was 
      performed on peripheral blood samples from 60 newly diagnosed patients with AML 
      and 40 healthy control subjects at Kasr Alainy School of Medicine, Cairo 
      University, from June 2016 to December 2017. The quantitative real-time 
      polymerase chain reaction (qRT-PCR) test was used to examine the relative 
      expression level of Cx43 and Cx32 genes in the patients and the control subjects. 
      The Chi square test or the Fisher exact test was employed to examine the 
      relationship between qualitative variables, while the independent t test or the 
      Mann-Whitney test was employed for quantitative data. All the tests were 
      two-tailed, and a P value of less than 0.05 was considered significant. RESULTS: 
      Among the patients with AML, 65% had a high Cx32 expression level, whereas 63.3% 
      had a low Cx43 expression level. There was a statistically significant difference 
      in the fold change values of Cx32 and Cx43 expression between the patient group 
      and the control group (P=0.009 vs P=0.013, respectively). There was a remarkable 
      association between both Cxs and CD34 and HLA-DR cells. CONCLUSION: Cx expression 
      in samples may add to the diagnostic workup of AML. Although we found a negative 
      correlation between Cx43 expression and the peripheral blood blast percentage, 
      the response after the first induction of chemotherapy showed no significant 
      relationship with Cx43 and Cx32.
CI  - Copyright: (c) Iranian Journal of Medical Sciences.
FAU - Fateen, Mohamed
AU  - Fateen M
AD  - Department of Clinical and Chemical Pathology, Cairo University, Cairo, Egypt.
FAU - Seif, Alia
AU  - Seif A
AD  - Department of Clinical and Chemical Pathology, Cairo University, Cairo, Egypt.
FAU - Salama, Rasha
AU  - Salama R
AD  - Department of Clinical Oncology, Cairo University, Cairo, Egypt.
FAU - Shams, Ahmed
AU  - Shams A
AD  - Department of Clinical and Chemical Pathology, Cairo University, Cairo, Egypt.
FAU - Amin, Dalia
AU  - Amin D
AD  - Department of Clinical and Chemical Pathology, Cairo University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Med Sci
JT  - Iranian journal of medical sciences
JID - 8104374
RN  - 0 (Connexin 43)
RN  - 0 (Connexins)
RN  - 0 (GJA1 protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Connexin 43/*analysis/genetics
MH  - Connexins/*analysis/genetics
MH  - Cross-Sectional Studies
MH  - Egypt
MH  - Female
MH  - Humans
MH  - Leukemia, Myeloid, Acute/*genetics
MH  - Male
MH  - Middle Aged
MH  - Risk Factors
MH  - Gap Junction beta-1 Protein
PMC - PMC8438339
OTO - NOTNLM
OT  - Gap junctions
OT  - Gene expression
OT  - Leukemia, Myeloid, Acute
OT  - Connexin 43
EDAT- 2021/09/21 06:00
MHDA- 2021/10/27 06:00
PMCR- 2021/09/01
CRDT- 2021/09/20 05:53
PHST- 2020/01/15 00:00 [received]
PHST- 2020/06/13 00:00 [revised]
PHST- 2020/08/01 00:00 [accepted]
PHST- 2021/09/20 05:53 [entrez]
PHST- 2021/09/21 06:00 [pubmed]
PHST- 2021/10/27 06:00 [medline]
PHST- 2021/09/01 00:00 [pmc-release]
AID - IJMS-46-5 [pii]
AID - 10.30476/ijms.2020.84511.1477 [doi]
PST - ppublish
SO  - Iran J Med Sci. 2021 Sep;46(5):347-354. doi: 10.30476/ijms.2020.84511.1477.