PMID- 34539654 OWN - NLM STAT- MEDLINE DCOM- 20211222 LR - 20220310 IS - 1664-3224 (Electronic) IS - 1664-3224 (Linking) VI - 12 DP - 2021 TI - Circulating Placental Vesicles Carry HLA-DR in Pre-Eclampsia: A New Potential Marker of the Syndrome. PG - 717879 LID - 10.3389/fimmu.2021.717879 [doi] LID - 717879 AB - BACKGROUND: Pre-eclampsia (PE) is a common disorder of pregnancy that usually presents with hypertension and proteinuria. The clinical presentation arises from soluble factors released into the maternal circulation from the placenta owing to the stress of syncytiotrophoblast, consequence of defective placentation occurring in the first half of pregnancy. Reduced tolerance of the semiallogeneic fetus by the maternal immune system has been proposed as first trigger leading to poor placentation. We previously observed aberrant expression of human leukocyte antigen (HLA)-DR molecules in the syncytiotrophoblast of a subset of women with PE. Aim of this study was to investigate abnormal expression of circulating HLA-DR in syncytiotrophoblast-derived extracellular vesicles (STBEVs) in women with PE compared to normal pregnant women. METHODS: peripheral venous blood was collected from 22 women with PE and 22 normal pregnant women. Circulating STBEVs were collected by ultra-centrifugation (120000 g) and analyzed for the expression of HLA-DR and placental alkaline phosphatase (PLAP), a specific marker of the placenta, by Western blot analysis and flow cytometry. RESULTS: circulating STBEVs positive for HLA-DR were observed in 64% of PE women while no HLA-DR positivity was detected in any of the controls (P<0.01). CONCLUSIONS: Aberrant expression of HLA-DR in circulating STBEVs is specifically associated to PE. Further studies are required: a) to define the role of aberrant placental expression of HLA-DR molecules in the pathogenesis of PE; b) evaluate a possible application of detecting circulating HLA-DR positive STBEVs in the diagnosis and prediction of PE in the first and second trimester of pregnancy. CI - Copyright (c) 2021 Tersigni, Lucchetti, Franco, Colella, Neri, Crispino, Sgambato, Lanzone, Scambia, Vatish and Di Simone. FAU - Tersigni, Chiara AU - Tersigni C AD - Unita Operativa Complessa di Ostetricia e Patologia Ostetrica, Dipartimento di Scienze della Salute della Donna e del Bambino e di Sanita Pubblica, Fondazione Policlinico Universitario A. Gemelli, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Lucchetti, Donatella AU - Lucchetti D AD - Dipartimento Universitario di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Franco, Rita AU - Franco R AD - Istituto di Clinica Ostetrica e Ginecologica, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Colella, Filomena AU - Colella F AD - Dipartimento Universitario di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Neri, Caterina AU - Neri C AD - Unita Operativa Complessa di Ostetricia e Patologia Ostetrica, Dipartimento di Scienze della Salute della Donna e del Bambino e di Sanita Pubblica, Fondazione Policlinico Universitario A. Gemelli, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Crispino, Laura AU - Crispino L AD - Istituto di Clinica Ostetrica e Ginecologica, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Sgambato, Alessandro AU - Sgambato A AD - Dipartimento Universitario di Medicina e Chirurgia Traslazionale, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Lanzone, Antonio AU - Lanzone A AD - Unita Operativa Complessa di Ostetricia e Patologia Ostetrica, Dipartimento di Scienze della Salute della Donna e del Bambino e di Sanita Pubblica, Fondazione Policlinico Universitario A. Gemelli, Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. AD - Istituto di Clinica Ostetrica e Ginecologica, Universita Cattolica del Sacro Cuore, Rome, Italy. FAU - Scambia, Giovanni AU - Scambia G AD - Istituto di Clinica Ostetrica e Ginecologica, Universita Cattolica del Sacro Cuore, Rome, Italy. AD - Unita Operativa Complessa di Ginecologia Oncologica, Dipartimento di Scienze della Salute della Donna e del Bambino e di Sanita Pubblica, Fondazione Policlinico Universitario A. Gemelli Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS), Rome, Italy. FAU - Vatish, Manu AU - Vatish M AD - Nuffield Department of Women's & Reproductive Health, University of Oxford, Oxford, United Kingdom. FAU - Di Simone, Nicoletta AU - Di Simone N AD - Department of Biomedical Sciences, Humanitas University, Milan, Italy. AD - Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Humanitas Research Hospital, Rozzano, Milan, Italy. LA - eng GR - MR/J003360/1/MRC_/Medical Research Council/United Kingdom PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20210903 PL - Switzerland TA - Front Immunol JT - Frontiers in immunology JID - 101560960 RN - 0 (Biomarkers) RN - 0 (HLA-DR Antigens) SB - IM MH - Adult MH - *Biomarkers/blood MH - Disease Susceptibility MH - Extracellular Vesicles/*metabolism MH - Female MH - Flow Cytometry MH - HLA-DR Antigens/*immunology/metabolism MH - Humans MH - Placenta/*immunology/*metabolism MH - Pre-Eclampsia/blood/etiology/*metabolism MH - Pregnancy MH - Prognosis PMC - PMC8446281 OTO - NOTNLM OT - human leukocyte antigen OT - placenta OT - pre-eclampsia OT - pregnancy OT - syncytiotrophoblast extracellular vesicles COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2021/09/21 06:00 MHDA- 2021/12/24 06:00 PMCR- 2021/01/01 CRDT- 2021/09/20 06:02 PHST- 2021/05/31 00:00 [received] PHST- 2021/08/16 00:00 [accepted] PHST- 2021/09/20 06:02 [entrez] PHST- 2021/09/21 06:00 [pubmed] PHST- 2021/12/24 06:00 [medline] PHST- 2021/01/01 00:00 [pmc-release] AID - 10.3389/fimmu.2021.717879 [doi] PST - epublish SO - Front Immunol. 2021 Sep 3;12:717879. doi: 10.3389/fimmu.2021.717879. eCollection 2021.