PMID- 34540004
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210921
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 13
IP  - 8
DP  - 2021
TI  - CircRNA LRP6 promotes high-glucose induced proliferation and migration of 
      vascular smooth muscle cells through regulating miR-545-3p/HMGA1 signaling axis.
PG  - 8909-8920
AB  - OBJECTIVE: The dysfunction of vascular smooth muscle cells (VSMCs) has been 
      revealed to be closely linked with the pathogenesis of cardiovascular diseases in 
      diabetes. Recently, circular RNAs (circRNAs) were found to regulate the behaviors 
      of VSMCs. Here, we attempted to study the role of circLRP6 in VSMCs under 
      diabetes condition. METHODS: Human VSMCs were cultured under the condition of 
      normal glucose (NG) or high glucose (HG). VSMC viability and proliferation were 
      estimated by CCK-8 and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assays. VSMC 
      migration and invasion were assessed via wound-healing and transwell experiments. 
      Protein expression of HMGA1 was measured in VSMCs using western blot and 
      immunofluorescence analysis. Relative expressions of circLRP6, miR-545-3p, and 
      HMGA1 mRNA were estimated in VSMCs using qRT-PCR. The co-localization of circLRP6 
      and miR-545-3p was verified by fluorescence in situ hybridization (FISH) 
      analysis. Binding sequence of miR-545-3p in circLRP6 or HMGA1 was predicted using 
      StarBase tool, and verified by RNA immunoprecipitation and dual-luciferase 
      reporter experiments. RESULTS: HG exposure promoted VSMC proliferation, migration 
      and invasion, upregulated the circLRP6 expression, and downregulated HMGA1 
      expression. Knockdown of circLRP6 or overexpression of miR-545-3p abrogated the 
      HG-caused VSMC proliferation, migration and invasion. CircLRP6 severed as a 
      miR-545-3p sponge, and HMGA1 was targeted by miR-545-3p. MiR-545-3p inhibitor 
      blocked the suppressive effects of si-circLRP6 on VSMC in the presence of HG. 
      CONCLUSION: These findings suggest that circRNA LRP6 promotes HG-induced VSMC 
      proliferation and migration through regulating miR-545-3p/HMGA1 signaling axis.
CI  - AJTR Copyright (c) 2021.
FAU - Bai, Yuanyuan
AU  - Bai Y
AD  - Department of Geriatrics, National Key Clinical Specialty, Guangzhou First 
      People's Hospital, School of Medicine, South China University of Technology 
      Guangzhou 510180, Guangdong, China.
FAU - Liu, Feng
AU  - Liu F
AD  - Department of Geriatrics, National Key Clinical Specialty, Guangzhou First 
      People's Hospital, School of Medicine, South China University of Technology 
      Guangzhou 510180, Guangdong, China.
FAU - Yang, Zili
AU  - Yang Z
AD  - Department of Geriatrics, National Key Clinical Specialty, Guangzhou First 
      People's Hospital, School of Medicine, South China University of Technology 
      Guangzhou 510180, Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20210815
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC8430135
OTO - NOTNLM
OT  - HMGA1
OT  - Vascular smooth muscle cells
OT  - circLRP6
OT  - high glucose
OT  - miR-545-3p
COIS- None.
EDAT- 2021/09/21 06:00
MHDA- 2021/09/21 06:01
PMCR- 2021/08/15
CRDT- 2021/09/20 06:06
PHST- 2021/04/21 00:00 [received]
PHST- 2021/06/11 00:00 [accepted]
PHST- 2021/09/20 06:06 [entrez]
PHST- 2021/09/21 06:00 [pubmed]
PHST- 2021/09/21 06:01 [medline]
PHST- 2021/08/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2021 Aug 15;13(8):8909-8920. eCollection 2021.