PMID- 34540044
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210921
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 13
IP  - 8
DP  - 2021
TI  - miR-497-5p induces apoptosis in K562 cells by downregulating ROCK1.
PG  - 9278-9284
AB  - OBJECTIVE: To validate the role of miR-497-5p in apoptosis in K562 cells by 
      targeting Rho-associated kinase isoform 1 (ROCK1). METHODS: From January, 2017 to 
      February, 2019, 57 patients with chronic myeloid leukemia (CML) treated in our 
      hospital were included in patient group, and 50 healthy individuals were 
      recruited as control group. miR-497-5p level in peripheral blood was quantitated 
      using qRT-PCR. After transfecting with miR-497-5p overexpression vector and ROCK1 
      inhibitor, K562 cells were monitored in terms of proliferation (CCK8 assay), 
      migration and invasion (Transwell), and apoptosis (flow cytometry). Binding loci 
      between miR-497-5p and ROCK1 were predicted, and the targeting relationship was 
      confirmed (dual-luciferase reporter (DLR) assay). RESULTS: miR-497-5p was poorly 
      expressed in CML (P < 0.05). Forced overexpression of miR-497-5p or inhibition of 
      ROCK1 suppressed malignant processes (proliferation, proliferation, migration and 
      invasion) in K562 cells and induced apoptosis (P < 0.05). DLR assay revealed a 
      decreased luciferase activity after miR-497-5p binding to ROCK1 (P < 0.05). 
      CONCLUSION: miR-497-5p induces apoptosis in K562 cells by downregulating ROCK1.
CI  - AJTR Copyright (c) 2021.
FAU - Chen, Nafei
AU  - Chen N
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Meng, Zhen
AU  - Meng Z
AD  - Department of Hematology, Hudson International Peace Hospital, Heng Shui City 
      People's Hospital Hengshui 053000, Hebei Province, China.
FAU - Song, Jiaojie
AU  - Song J
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Kong, Lingfang
AU  - Kong L
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Zhang, Yehua
AU  - Zhang Y
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Guo, Suli
AU  - Guo S
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Zhang, Xiaokun
AU  - Zhang X
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Lu, Xin
AU  - Lu X
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Jiang, Licai
AU  - Jiang L
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Chen, Ran
AU  - Chen R
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Jiao, Zongjiu
AU  - Jiao Z
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
FAU - Zhao, Liyun
AU  - Zhao L
AD  - Department of Hematology, Xingtai People's Hospital Xingtai 054000, Hebei 
      Province, China.
LA  - eng
PT  - Journal Article
DEP - 20210815
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC8430193
OTO - NOTNLM
OT  - K562 cells
OT  - ROCK1
OT  - apoptosis
OT  - invasion and migration
OT  - miR-497-5p
COIS- None.
EDAT- 2021/09/21 06:00
MHDA- 2021/09/21 06:01
PMCR- 2021/08/15
CRDT- 2021/09/20 06:06
PHST- 2020/10/22 00:00 [received]
PHST- 2020/12/11 00:00 [accepted]
PHST- 2021/09/20 06:06 [entrez]
PHST- 2021/09/21 06:00 [pubmed]
PHST- 2021/09/21 06:01 [medline]
PHST- 2021/08/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2021 Aug 15;13(8):9278-9284. eCollection 2021.