PMID- 34541823
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 1582-4934 (Electronic)
IS  - 1582-1838 (Print)
IS  - 1582-1838 (Linking)
VI  - 25
IP  - 20
DP  - 2021 Oct
TI  - LINC00261 elevation inhibits angiogenesis and cell cycle progression of 
      pancreatic cancer cells by upregulating SCP2 via targeting FOXP3.
PG  - 9826-9836
LID - 10.1111/jcmm.16930 [doi]
AB  - Long non-coding RNAs (lncRNAs) biological functions and molecular mechanisms 
      associated with pancreatic cancer (PC) remain to be poorly elucidated. We aimed 
      to clarify the role of lncRNA LINC00261 (LINC00261) in PC and confirm its 
      regulatory mechanisms. Bioinformatics analysis, RNA pull-down and RIP assays were 
      performed to investigate relationship between LINC00261 and forkhead box P3 
      (FOXP3). Further, dual-luciferase reporter gene and ChIP assays were employed to 
      confirm the relationship among LINC00261, FOXP3 and sterol carrier protein-2 
      (SCP2). PC cells were introduced with a series of vectors to verify the effects 
      of LINC00261 and SCP2 on the viability, cell cycle progression, migration and 
      angiogenesis of PC cells. Nude mice with the xenograft tumour were used to 
      evaluate the effects LINC00261 on the tumourigenicity. LINC00261 was lowly 
      expressed in PC tissues and cells. SCP2 was inhibited by LINC00261 through FOXP3. 
      Functionally, upregulated LINC00261 or downregulated SCP2 led to reduced cell 
      viability, migration, angiogenesis and tumourigenicity potentials. This study 
      demonstrated the inhibitory role of LINC00261 in the angiogenesis and cell cycle 
      progression of PC cells. It acts through the negative regulation of SCP2 via 
      targeting FOXP3. Findings in this study highlight a potentially biomarker for PC 
      treatment.
CI  - (c) 2021 The Authors. Journal of Cellular and Molecular Medicine published by 
      Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.
FAU - Zou, Jun
AU  - Zou J
AD  - Department of abdominal surgery, Jiangxi Cancer Hospital, Nanchang, China.
FAU - Pei, Xuanzeng
AU  - Pei X
AD  - Department of General Surgery, Affiliated Hospital of Jiaxing University, 
      Jiaxing, China.
FAU - Xing, Dan
AU  - Xing D
AD  - Department of General Surgery, Affiliated Hospital of Jiaxing University, 
      Jiaxing, China.
FAU - Wu, Xiaojun
AU  - Wu X
AD  - Department of General Surgery, Affiliated Hospital of Jiaxing University, 
      Jiaxing, China.
FAU - Chen, Shuai
AU  - Chen S
AUID- ORCID: 0000-0002-8326-0056
AD  - Department of General Surgery, Affiliated Hospital of Jiaxing University, 
      Jiaxing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210919
PL  - England
TA  - J Cell Mol Med
JT  - Journal of cellular and molecular medicine
JID - 101083777
RN  - 0 (Carrier Proteins)
RN  - 0 (FOXP3 protein, human)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (long non-coding RNA 00261, human)
RN  - 0 (sterol carrier proteins)
SB  - IM
MH  - Animals
MH  - Carrier Proteins/*genetics
MH  - Cell Cycle/*genetics
MH  - Cell Line, Tumor
MH  - Computational Biology/methods
MH  - Disease Models, Animal
MH  - Forkhead Transcription Factors/*genetics/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Heterografts
MH  - Humans
MH  - Immunohistochemistry
MH  - Mice
MH  - Neovascularization, Pathologic/*genetics
MH  - Pancreatic Neoplasms/*genetics/metabolism/*pathology
MH  - RNA Interference
MH  - RNA, Long Noncoding/*genetics
PMC - PMC8505824
OTO - NOTNLM
OT  - angiogenesis
OT  - cell cycle progression
OT  - forkhead box P3
OT  - long non-coding RNA LINC00261
OT  - pancreatic cancer
OT  - sterol carrier protein-2
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2021/09/21 06:00
MHDA- 2022/02/26 06:00
PMCR- 2021/10/01
CRDT- 2021/09/20 06:39
PHST- 2021/07/29 00:00 [revised]
PHST- 2021/02/07 00:00 [received]
PHST- 2021/09/01 00:00 [accepted]
PHST- 2021/09/21 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2021/09/20 06:39 [entrez]
PHST- 2021/10/01 00:00 [pmc-release]
AID - JCMM16930 [pii]
AID - 10.1111/jcmm.16930 [doi]
PST - ppublish
SO  - J Cell Mol Med. 2021 Oct;25(20):9826-9836. doi: 10.1111/jcmm.16930. Epub 2021 Sep 
      19.