PMID- 34544905
OWN - NLM
STAT- MEDLINE
DCOM- 20220202
LR  - 20220202
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 13
IP  - 18
DP  - 2021 Sep 20
TI  - Key genes affecting the progression of nasopharyngeal carcinoma identified by 
      RNA-sequencing and bioinformatic analysis.
PG  - 22176-22187
LID - 10.18632/aging.203521 [doi]
AB  - BACKGROUND: The present work was conducted to screen the potential biomarkers 
      affecting nasopharyngeal carcinoma (NPC) progression through RNA-sequencing 
      (RNA-seq), bioinformatic analysis and functional experiments. MATERIALS AND 
      METHODS: Six normal samples and five NPC clinical samples were collected for 
      RNA-seq analysis. The expression levels in both groups were determined through 
      student's t-test. We identified genes of P < 0.01 as the differentially expressed 
      genes (DEGs). In addition, gene set enrichment analysis (GSEA) was conducted. 
      Afterwards, STRING V10 database was employed to extract protein interactions 
      among the DEGs. Later, we established a protein-protein interaction (PPI) 
      network, and used the Cytoscape software for network visualization. qRT-PCR was 
      conducted to verify hub genes from clinical samples. Then, the function of CXCL10 
      in cell proliferation, apoptosis, invasion and migration was evaluated. RESULTS: 
      A total of 2024 DEGs were identified, among which, 1449 were down-regulated and 
      575 were up-regulated. The PPI was constructed, and the hub genes including 
      Insulin Like Growth Factor 1 (IGF1), C-X-C Motif Chemokine Ligand 10 (CXCL10), 
      Interleukin 13 (IL13), Intercellular Adhesion Molecule 1 (ICAM1), G Protein 
      Subunit Gamma Transducin 1 (GNGT1), Matrix Metallopeptidase 1 (MMP1), Neurexin 1 
      (NRXN1) and Matrix Metallopeptidase 3 (MMP3) were obtained. The expression levels 
      of CXCL10, IGF1, MMP3, MMP1, ICAM1, and IL-13 were significantly up-regulated in 
      tumor tissues. High expression levels of CXCL10, MMP3 and ICAM1 predicted poor 
      prognosis of NPC patients. CXCL10 silencing suppressed NPC cell proliferation and 
      migration. CONCLUSIONS: CXCL10 may serve as a potential key gene affecting NPC 
      genesis and progression.
FAU - Wang, Yihong
AU  - Wang Y
AD  - Department of Otolaryngology, The First Affiliated Hospital of Soochow 
      University, Soochow 215000, Jiangsu Province, China.
FAU - Li, Manyi
AU  - Li M
AD  - Department of Otolaryngology, The First Affiliated Hospital of Soochow 
      University, Soochow 215000, Jiangsu Province, China.
FAU - Guo, Yan
AU  - Guo Y
AD  - Department of Otolaryngology, The First Affiliated Hospital of Soochow 
      University, Soochow 215000, Jiangsu Province, China.
FAU - Huang, Haiping
AU  - Huang H
AD  - Department of Otolaryngology, The First Affiliated Hospital of Soochow 
      University, Soochow 215000, Jiangsu Province, China.
FAU - Dong, Xuelin
AU  - Dong X
AD  - Department of Otolaryngology, The First Affiliated Hospital of Soochow 
      University, Soochow 215000, Jiangsu Province, China.
FAU - Sun, Yangguang
AU  - Sun Y
AD  - Department of Otolaryngology, The First Affiliated Hospital of Soochow 
      University, Soochow 215000, Jiangsu Province, China.
FAU - Liu, Jisheng
AU  - Liu J
AD  - Department of Otolaryngology, The First Affiliated Hospital of Soochow 
      University, Soochow 215000, Jiangsu Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210920
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Chemokine CXCL10)
RN  - 0 (Interleukin-13)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Chemokine CXCL10/genetics/metabolism
MH  - Computational Biology
MH  - Disease Progression
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Regulatory Networks
MH  - Humans
MH  - Interleukin-13/genetics/metabolism
MH  - Matrix Metalloproteinase 1/genetics/metabolism
MH  - Matrix Metalloproteinase 3/genetics/metabolism
MH  - Nasopharyngeal Carcinoma/*genetics/metabolism/pathology
MH  - Nasopharyngeal Neoplasms/*genetics/metabolism/pathology
MH  - RNA-Seq
PMC - PMC8507278
OTO - NOTNLM
OT  - differentially expressed genes
OT  - nasopharyngeal carcinoma
OT  - progression
OT  - protein encoding genes
OT  - protein-protein interaction network
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest related to 
      this study.
EDAT- 2021/09/22 06:00
MHDA- 2022/02/03 06:00
PMCR- 2021/09/30
CRDT- 2021/09/21 05:50
PHST- 2020/11/13 00:00 [received]
PHST- 2021/08/02 00:00 [accepted]
PHST- 2021/09/22 06:00 [pubmed]
PHST- 2022/02/03 06:00 [medline]
PHST- 2021/09/21 05:50 [entrez]
PHST- 2021/09/30 00:00 [pmc-release]
AID - 203521 [pii]
AID - 10.18632/aging.203521 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2021 Sep 20;13(18):22176-22187. doi: 10.18632/aging.203521. 
      Epub 2021 Sep 20.