PMID- 34546332
OWN - NLM
STAT- MEDLINE
DCOM- 20220308
LR  - 20230208
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 157
IP  - 3
DP  - 2022 Mar 3
TI  - Maternal vs Fetal Origin of Placental Intervillous Thrombi.
PG  - 365-373
LID - 10.1093/ajcp/aqab139 [doi]
AB  - OBJECTIVES: To determine maternal vs fetal origin for blood in placental 
      intervillous thrombi (IVTs). METHODS: We used comparative analysis of 
      microsatellites (short tandem repeats [STRs]), sex chromosome fluorescence in 
      situ hybridization (FISH), and immunohistochemistry (IHC) for fetal 
      (a-fetoprotein [AFP]) and maternal (immunoglobulin M [IgM]) serum proteins to 
      distinguish the origin of IVTs. Using an informatics approach, we tested the 
      association between IVTs and fetomaternal hemorrhage (FMH). RESULTS: In 9 of 10 
      cases, the preponderance of evidence showed that the thrombus was mostly or 
      entirely maternal in origin. In 1 case, the thrombus was of mixed origins. STR 
      testing was prone to contamination by entrapped fetal villi. FISH was useful but 
      limited only to cases with male fetuses. IgM showed stronger staining than AFP in 
      9 cases, supporting maternal origin. By informatics, we found no association 
      between IVTs and FMH. CONCLUSIONS: Evidence supports a maternal origin for blood 
      in IVTs. IHC for IgM and AFP may be clinically useful in determining maternal vs 
      fetal contribution to IVTs.
CI  - (c) American Society for Clinical Pathology, 2021. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Sukhanova, Madina
AU  - Sukhanova M
AD  - Departments of Pathology, Chicago, IL, USA.
FAU - Mithal, Leena B
AU  - Mithal LB
AD  - Pediatrics (Infectious Disease), Chicago, IL, USA.
AD  - Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
FAU - Otero, Sebastian
AU  - Otero S
AD  - Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
FAU - Azad, Hooman A
AU  - Azad HA
AD  - Obstetrics and Gynecology (Maternal-Fetal Medicine), Northwestern University 
      Feinberg School of Medicine, Chicago, IL, USA.
FAU - Miller, Emily S
AU  - Miller ES
AD  - Anne and Robert H. Lurie Children's Hospital, Chicago, IL, USA.
FAU - Jennings, Lawrence J
AU  - Jennings LJ
AD  - Departments of Pathology, Chicago, IL, USA.
FAU - Shanes, Elisheva D
AU  - Shanes ED
AD  - Departments of Pathology, Chicago, IL, USA.
FAU - Goldstein, Jeffery A
AU  - Goldstein JA
AUID- ORCID: 0000-0002-4086-057X
AD  - Departments of Pathology, Chicago, IL, USA.
LA  - eng
GR  - P30 CA060553/CA/NCI NIH HHS/United States
GR  - UL1 TR001422/TR/NCATS NIH HHS/United States
GR  - NCI CA060553/NH/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
SB  - IM
MH  - Female
MH  - Fetus
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - *Placenta
MH  - Pregnancy
MH  - *Thrombosis/genetics
PMC - PMC8500002
OTO - NOTNLM
OT  - COVID-19
OT  - FISH
OT  - Immunohistochemistry
OT  - Perinatal
OT  - Placenta
OT  - STR
OT  - Thrombi
EDAT- 2021/09/22 06:00
MHDA- 2022/03/09 06:00
PMCR- 2022/09/21
CRDT- 2021/09/21 12:39
PHST- 2021/03/13 00:00 [received]
PHST- 2021/07/22 00:00 [accepted]
PHST- 2021/09/22 06:00 [pubmed]
PHST- 2022/03/09 06:00 [medline]
PHST- 2021/09/21 12:39 [entrez]
PHST- 2022/09/21 00:00 [pmc-release]
AID - 6373364 [pii]
AID - aqab139 [pii]
AID - 10.1093/ajcp/aqab139 [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2022 Mar 3;157(3):365-373. doi: 10.1093/ajcp/aqab139.