PMID- 34547619
OWN - NLM
STAT- MEDLINE
DCOM- 20211112
LR  - 20211112
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Print)
IS  - 0032-5791 (Linking)
VI  - 100
IP  - 11
DP  - 2021 Nov
TI  - Factors affecting gene expression associated with the skin color of black-bone 
      chicken in Thailand.
PG  - 101440
LID - S0032-5791(21)00463-6 [pii]
LID - 10.1016/j.psj.2021.101440 [doi]
LID - 101440
AB  - The objective of this study was to investigate the effect of breed, sex, and age 
      on the gene expression level of melanocortin 1 receptor (MC1R), DOPA chrome 
      tautomerase (DCT), tyrosinase-related protein 1 (TYRP1), tyrosinase (TYR), and 
      agouti signaling protein (ASIP) genes in Thai commercial chicken lines. All 
      chicken have received Newscastle vaccination, and no antibiotics or any drugs 
      were used in this study. Four chicken breeds including Black-Chinese, KU-Phuparn, 
      Sri Mok, and Pradu Hang Dam were used in this study. These breeds can be 
      classified by their skin color into 3 group including black (Black Chinese and 
      KU-Phuparn), light black (Sri Mok), and yellowish white (Pradu Hang Dam). One 
      hundred chickens per breed were used in this study. Breast skin tissue was 
      randomly collected from 8 chickens (4 males, 4 females) per breed at 4, 8, 12, 
      and 16 wk of age. The mRNA expression was analyzed using qRT-PCR and the gene 
      expression level was calculated as 2(-DeltaDeltaCT). From the results, breed 
      significantly (P < 0.01) affected the expression level for the 5 genes evaluated. 
      Birds with the black skin color had greater TYRP1 and TYR gene expression when 
      compared to chickens with light black and yellowish-white skin color, 
      respectively. Whereas, chickens with yellowish-white skin color had greater ASIP 
      gene expression when compared to chickens having the other skin colors. Sex 
      significantly affected DCT, TYRP1, and TYR gene expression where the gene 
      expression in males was greater when compared to females (P < 0.05). Age affected 
      all gene expression levels (P < 0.01). At 4 wk of age, MC1R, DCT, TYRP1, and TYR 
      gene expression was the highest and decreased as bird age increased (P < 0.05); 
      however, ASIP gene expression was greatest at 8 wk of age. After 8 wk of age all 
      gene expression for the genes evaluated in this study decreased as age increased. 
      In addition, an interaction between breed and sex (P < 0.05) impacted DCT and 
      ASIP gene expression. The results from this study showed that all genes evaluated 
      can be used as candidate markers to further improve the blackness of the 
      chicken's skin because the most desired skin color is black in the Thai 
      black-bone chicken population.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Khumpeerawat, Panuwat
AU  - Khumpeerawat P
AD  - Department of Animal Science, Faculty of Agricultural, Khon Kaen University, 
      Mueang Khon Kaen 40000, Thailand.
FAU - Duangjinda, Monchai
AU  - Duangjinda M
AD  - Department of Animal Science, Faculty of Agricultural, Khon Kaen University, 
      Mueang Khon Kaen 40000, Thailand; Network Center for Animal Breeding and Omics 
      Research, Faculty of Agricultural, Khon Kaen University, Mueang Khon Kaen 40000, 
      Thailand. Electronic address: monchai@kku.ac.th.
FAU - Phasuk, Yupin
AU  - Phasuk Y
AD  - Department of Animal Science, Faculty of Agricultural, Khon Kaen University, 
      Mueang Khon Kaen 40000, Thailand; Network Center for Animal Breeding and Omics 
      Research, Faculty of Agricultural, Khon Kaen University, Mueang Khon Kaen 40000, 
      Thailand.
LA  - eng
PT  - Journal Article
DEP - 20210819
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Agouti Signaling Protein)
SB  - IM
MH  - Agouti Signaling Protein/genetics
MH  - Animals
MH  - *Chickens/genetics
MH  - Color
MH  - Female
MH  - Gene Expression
MH  - Male
MH  - *Skin Pigmentation/genetics
MH  - Thailand
PMC - PMC8463778
OTO - NOTNLM
OT  - black-bone chicken
OT  - factor
OT  - gene expression
OT  - skin color
EDAT- 2021/09/22 06:00
MHDA- 2021/11/16 06:00
PMCR- 2021/08/19
CRDT- 2021/09/21 20:24
PHST- 2020/09/24 00:00 [received]
PHST- 2021/07/03 00:00 [revised]
PHST- 2021/08/15 00:00 [accepted]
PHST- 2021/09/22 06:00 [pubmed]
PHST- 2021/11/16 06:00 [medline]
PHST- 2021/09/21 20:24 [entrez]
PHST- 2021/08/19 00:00 [pmc-release]
AID - S0032-5791(21)00463-6 [pii]
AID - 101440 [pii]
AID - 10.1016/j.psj.2021.101440 [doi]
PST - ppublish
SO  - Poult Sci. 2021 Nov;100(11):101440. doi: 10.1016/j.psj.2021.101440. Epub 2021 Aug 
      19.