PMID- 34547631
OWN - NLM
STAT- MEDLINE
DCOM- 20211012
LR  - 20220531
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 175
DP  - 2021 Nov
TI  - Increasing of matrix metalloproteinase 3 in bovine endometritis.
PG  - 83-88
LID - S0093-691X(21)00315-0 [pii]
LID - 10.1016/j.theriogenology.2021.09.001 [doi]
AB  - Matrix metalloproteinase 3 (MMP3), a key member of the MMPs family, is involved 
      in the modulation of endometrial inflammation and innate immunity. However, the 
      role of MMP3 in bovine endometritis remains unknown. To investigate the role of 
      MMP3 in bovine endometritis, endometrial MMP3 expression were determined in 
      uterine biopsies from twenty 40-60 days postpartum dairy cows, which six were 
      healthy cows and fourteen were endometritic cows. Moreover, MMP3 expression were 
      also detected at different intensity of inflammatory response, which was induced 
      by graded concentrations (0, 1, 5, 10 mug/ml) of LPS in bovine endometrial 
      epithelial cells (BEECs) in vitro. RT-qPCR was used to test the mRNA levels of 
      MMP3 in tissues or cells. Western blot was conducted to measure protein levels, 
      and enzyme-linked immunosorbent assay (ELISA) was used for TNF-alpha and IL-1beta in 
      cell supernatant. Results showed that MMP3 mRNA and protein levels significantly 
      increased and positive correlative with severity of endometritis in vivo. 
      Likewise, MMP3 expression also positive correlative with intensity of LPS 
      inflammatory response in BEECs in vitro. These results indicate that increasing 
      of MMP3 directly correlates with bovine endometritis, and its increasing may 
      contribute to progression of bovine endometritis.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Zhang, Shidong
AU  - Zhang S
AD  - Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou, 
      730050, PR China. Electronic address: sdzhang2006@126.com.
FAU - Wang, Dongsheng
AU  - Wang D
AD  - Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou, 
      730050, PR China.
FAU - Yan, Zuoting
AU  - Yan Z
AD  - Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou, 
      730050, PR China; Key Lab of Veterinary Pharmaceutical Development of Ministry of 
      Agriculture, Lanzhou, 730050, PR China.
LA  - eng
PT  - Journal Article
DEP - 20210904
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 0 (Cytokines)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Cattle Diseases
MH  - Cytokines
MH  - *Endometritis/veterinary
MH  - Endometrium
MH  - Epithelial Cells
MH  - Female
MH  - *Matrix Metalloproteinase 3/genetics
OTO - NOTNLM
OT  - Bovine endometrial epithelial cells
OT  - Endometritis
OT  - Lipopolysaccharide
OT  - Matrix metalloproteinase 3
EDAT- 2021/09/22 06:00
MHDA- 2021/10/13 06:00
CRDT- 2021/09/21 20:24
PHST- 2021/05/20 00:00 [received]
PHST- 2021/09/02 00:00 [revised]
PHST- 2021/09/03 00:00 [accepted]
PHST- 2021/09/22 06:00 [pubmed]
PHST- 2021/10/13 06:00 [medline]
PHST- 2021/09/21 20:24 [entrez]
AID - S0093-691X(21)00315-0 [pii]
AID - 10.1016/j.theriogenology.2021.09.001 [doi]
PST - ppublish
SO  - Theriogenology. 2021 Nov;175:83-88. doi: 10.1016/j.theriogenology.2021.09.001. 
      Epub 2021 Sep 4.