PMID- 34551560
OWN - NLM
STAT- MEDLINE
DCOM- 20210924
LR  - 20210924
IS  - 1210-7875 (Print)
IS  - 1210-7875 (Linking)
VI  - 57
IP  - 3
DP  - 2021 Summer
TI  - Next generation sequencing - a science tool or routine pathology?
PG  - 136-143
AB  - Molecular assays for translocation detection in different tumors have gradually 
      been incorporated into routine diagnostics. However, conventional methods such as 
      fluorescence in situ hybridization (FISH) and reverse transcriptase-PCR come with 
      several drawbacks. Next-generation sequencing (NGS) can provide in-depth 
      detection of numerous gene alterations. The anchored multiplex PCR assay proved 
      to be a fast and easy-to-analyze approach for routine diagnostics laboratories. 
      Next-generation sequencing-based anchored multiplex PCR technique (Archer 
      FusionPlex Panels) is beneficial in both diagnosis for patient care and in 
      identification of a novel fusion breakpoint in tumors. NGS is useful in 
      identifying targetable molecular changes (point mutations, fusion genes, etc.) in 
      tumors that can serve as a rationale for inclusion of patients with advanced 
      disease in ongoing clinical trials and allow for better risk stratification.
FAU - Krskova, Lenka
AU  - Krskova L
FAU - Kalinova, Marketa
AU  - Kalinova M
FAU - Nemeckova, Tereza
AU  - Nemeckova T
FAU - Sipalova, Barbora
AU  - Sipalova B
FAU - Mrhalova, Marcela
AU  - Mrhalova M
FAU - Koblizek, Miroslav
AU  - Koblizek M
FAU - Balko, Jan
AU  - Balko J
FAU - Hacek, Jaromir
AU  - Hacek J
FAU - Novakova-Kodetova, Daniela
AU  - Novakova-Kodetova D
FAU - Vicha, Ales
AU  - Vicha A
FAU - Broz, Petr
AU  - Broz P
FAU - Zapotocky, Michal
AU  - Zapotocky M
FAU - Kabickova, Edita
AU  - Kabickova E
FAU - Zamecnik, Josef
AU  - Zamecnik J
LA  - eng
PT  - Journal Article
TT  - Sekvenovani nove generace - nastroj vědy či rutinni patologie?
PL  - Czech Republic
TA  - Cesk Patol
JT  - Ceskoslovenska patologie
JID - 0050734
SB  - IM
MH  - *High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Multiplex Polymerase Chain Reaction
MH  - *Neoplasms/diagnosis/genetics
MH  - Translocation, Genetic
OTO - NOTNLM
OT  - Fusion genes
OT  - next generation sequencing (NGS)
OT  - somatic mutations
OT  - targeted therapy
EDAT- 2021/09/24 06:00
MHDA- 2021/09/25 06:00
CRDT- 2021/09/23 05:29
PHST- 2021/09/23 05:29 [entrez]
PHST- 2021/09/24 06:00 [pubmed]
PHST- 2021/09/25 06:00 [medline]
AID - 128079 [pii]
PST - ppublish
SO  - Cesk Patol. 2021 Summer;57(3):136-143.