PMID- 3455164
OWN - NLM
STAT- MEDLINE
DCOM- 19881011
LR  - 20131121
IS  - 0884-0431 (Print)
IS  - 0884-0431 (Linking)
VI  - 2
IP  - 2
DP  - 1987 Apr
TI  - Rapid action of 1,25-dihydroxyvitamin D3 on calcium transport in perfused chick 
      duodenum: effect of inhibitors.
PG  - 99-107
AB  - Transport of 45Ca from the lumen to the venous effluent was studied in duodena of 
      normal, vitamin D3-replete (+D) chicks perfused through the celiac artery with 
      130 pM 1,25(OH)2D3 or vehicle. Administration of actinomycin D 3 h prior to 
      perfusion did not alter the unstimulated transport rate or diminish the response 
      to exogenous 1,25(OH)2D3: After 40 min exposure to the seco-steroid, 45Ca in the 
      vascular effluent was 140% of control levels. The anti-microfilament agent 
      cytochalasin b and the ionophore monensin, an inhibitor of Golgi function, 
      similarly failed to suppress 1,25(OH)2D3-stimulated calcium transport. In pilot 
      studies, Golgi and basal-lateral membrane fractions were prepared from duodenal 
      epithelium of vitamin D-deficient (-D) chicks treated with vehicle or 650 pmol of 
      1,25(OH)2D3 in vivo 2 h, 10 h, or 15 h before sacrifice, as well as from +D 
      birds. Analyses of Golgi fractions for cathepsin B (CB) activity revealed a 
      biphasic response with time, increasing to 200% of -D levels 2 h after 
      1,25(OH)2D3 administration and in equivalent preparations from +D birds. Less 
      pronounced increases in acid phosphatase activity were observed in the same 
      membrane fractions. In basal-lateral membranes, enhanced CB activity was 
      detectable 10 h after 1,25(OH)2D3 in vivo, rose to 155% of -D levels at 15 h, and 
      to 245% of controls in fractions from +D birds, whereas acid phosphatase was 75%, 
      81%, and 125% of controls, respectively, at these times.(ABSTRACT TRUNCATED AT 
      250 WORDS)
FAU - Nemere, I
AU  - Nemere I
AD  - Department of Biochemistry, University of California, Riverside 92521.
FAU - Norman, A W
AU  - Norman AW
LA  - eng
GR  - AM-09012-018/AM/NIADDK NIH HHS/United States
GR  - T32-AM-07310-04/AM/NIADDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - FXC9231JVH (Calcitriol)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Biological Transport, Active/drug effects
MH  - Calcitriol/*pharmacology
MH  - Calcium/*metabolism
MH  - Chickens
MH  - Duodenum/drug effects/*metabolism
EDAT- 1987/04/01 00:00
MHDA- 1987/04/01 00:01
CRDT- 1987/04/01 00:00
PHST- 1987/04/01 00:00 [pubmed]
PHST- 1987/04/01 00:01 [medline]
PHST- 1987/04/01 00:00 [entrez]
AID - 10.1002/jbmr.5650020204 [doi]
PST - ppublish
SO  - J Bone Miner Res. 1987 Apr;2(2):99-107. doi: 10.1002/jbmr.5650020204.