PMID- 34552818
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210925
IS  - 2162-2531 (Print)
IS  - 2162-2531 (Electronic)
IS  - 2162-2531 (Linking)
VI  - 26
DP  - 2021 Dec 3
TI  - Circular RNA circDNA2 upregulates CCDC6 expression to promote the progression of 
      gastric cancer via miR-149-5p suppression.
PG  - 360-373
LID - 10.1016/j.omtn.2021.05.021 [doi]
AB  - Circular (circ)RNAs are widely involved in gastric cancer (GC) pathogenesis, and 
      coiled-coil domain containing 6 (CCDC6) is a fused partner of multiple oncogenes; 
      however, the underlying mechanisms of how circRNAs regulate CCDC6 expression in 
      the progression and prognosis of GC remain unclear. Here, we discovered the 
      circRNA derived from the DNA2 gene locus (circDNA2) through RNA sequencing. By 
      performing quantitative real-time PCR and fluorescence in situ hybridization 
      (FISH) assays with a human tissue microarray, circDNA2 was found to be highly 
      expressed in GC tissues and associated with lymphatic invasion of GC patients. 
      Knockdown of circDNA2 expression suppressed the proliferation of GC cells by 
      reducing CCDC6 expression. Mechanistically, circDNA2 acted as a microRNA 
      (miR)-149-5p sponge, which was confirmed to target CCDC6 by RNA pulldown and 
      dual-luciferase reporter assays and rescue experiments. Both low miR-149-5p 
      expression and high CCDC6 expression were related to unfavorable prognosis in GC 
      patients. Moreover, GC patients with low miR-149-5p expression had shorter 
      overall survival and a higher risk of chemotherapy resistance than those with 
      high miR-149-5p expression. In summary, circDNA2 contributes to the growth and 
      lymphatic metastasis of GC by upregulating CCDC6 expression by sponging 
      miR-149-5p. The circDNA2/miR-149-5p/CCDC6 axis might be developed as a 
      therapeutic target and prognostic indicator for GC.
CI  - (c) 2021 The Authors.
FAU - Jin, Duochen
AU  - Jin D
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
AD  - First Clinical Medical College of Nanjing Medical University, Nanjing, China.
FAU - Huang, Keting
AU  - Huang K
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
AD  - First Clinical Medical College of Nanjing Medical University, Nanjing, China.
FAU - Peng, Lei
AU  - Peng L
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
FAU - Xu, Ping
AU  - Xu P
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
FAU - Dang, Yini
AU  - Dang Y
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
FAU - Yang, Jiajia
AU  - Yang J
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
FAU - Chen, Meihong
AU  - Chen M
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
FAU - Zhu, Xudong
AU  - Zhu X
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
AD  - First Clinical Medical College of Nanjing Medical University, Nanjing, China.
FAU - Wei, Shuchun
AU  - Wei S
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
AD  - First Clinical Medical College of Nanjing Medical University, Nanjing, China.
FAU - Yan, Jin
AU  - Yan J
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
AD  - First Clinical Medical College of Nanjing Medical University, Nanjing, China.
FAU - Zhang, Guoxin
AU  - Zhang G
AD  - Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical 
      University, No. 300 of Guangzhou Road, Nanjing 210029, China.
AD  - First Clinical Medical College of Nanjing Medical University, Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20210601
PL  - United States
TA  - Mol Ther Nucleic Acids
JT  - Molecular therapy. Nucleic acids
JID - 101581621
PMC - PMC8426470
OTO - NOTNLM
OT  - CCDC6
OT  - circular RNA circDNA2
OT  - gastric cancer
OT  - miR-149-5p
OT  - overall survival
COIS- The authors declare no competing interests.
EDAT- 2021/09/24 06:00
MHDA- 2021/09/24 06:01
PMCR- 2021/06/01
CRDT- 2021/09/23 06:52
PHST- 2020/11/11 00:00 [received]
PHST- 2021/05/26 00:00 [accepted]
PHST- 2021/09/23 06:52 [entrez]
PHST- 2021/09/24 06:00 [pubmed]
PHST- 2021/09/24 06:01 [medline]
PHST- 2021/06/01 00:00 [pmc-release]
AID - S2162-2531(21)00137-2 [pii]
AID - 10.1016/j.omtn.2021.05.021 [doi]
PST - epublish
SO  - Mol Ther Nucleic Acids. 2021 Jun 1;26:360-373. doi: 10.1016/j.omtn.2021.05.021. 
      eCollection 2021 Dec 3.