PMID- 34554377
OWN - NLM
STAT- MEDLINE
DCOM- 20220310
LR  - 20220310
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 57
IP  - 8
DP  - 2021 Sep
TI  - Integrated analysis of mRNA and long non-coding RNA expression profiles reveals 
      the potential roles of lncRNA-mRNA network in carp macrophage immune regulation.
PG  - 835-847
LID - 10.1007/s11626-021-00610-5 [doi]
AB  - Long non-coding RNAs (lncRNAs) have emerged as a hot topic in research as 
      mounting evidence has indicated their transcriptional or post-transcriptional 
      regulatory potential in multiple biological processes. Previous studies have 
      revealed the involvement of lncRNAs in the immunoregulation of mammalian 
      macrophages by changing mRNA expression; however, studies on the lncRNAs in fish 
      macrophages and their potential roles in the immune system remain unknown. 
      Primary macrophages were isolated from the head kidney (HK) of red common carp 
      (Cyprinus carpio) and high-throughput lncRNA-mRNA sequencing was performed using 
      the Illumina HiSeq platform. The results revealed that the most highly expressed 
      mRNAs in primary HK macrophages were mainly involved in immune-related signal 
      pathways. Furthermore, the most enriched immune-related GO term and KEGG pathway 
      of the mRNAs were "immune system development" and "chemokine signaling pathway," 
      respectively. A total of 20,333 lncRNAs, composed of 10,512 known and 9821 novel 
      lncRNAs, were identified, and functional enrichment analysis of the lncRNA-mRNA 
      network indicated that the expressed lncRNAs in primary HK macrophages could be 
      associated with the regulation of multiple immune-related signaling pathways. In 
      addition, the expressions of several selected lncRNAs and their related mRNAs 
      were determined in carp macrophages following a 6-h exposure to 
      lipopolysaccharide (LPS) and Poly(I: C), the results of which confirmed the 
      co-expression regulation of lncRNAs and target mRNAs in the immune response of 
      carp macrophages. These results suggest the correlative of the lncRNA-mRNA 
      network in fish macrophage immune response, which may further affect the 
      cross-talk of various signaling pathways by interaction with other network genes. 
      Here, we provided fundamental data about the transcriptome profiles of primary HK 
      macrophages from red common carp by analysis of the lncRNA-mRNA network, and 
      ultimately suggest the potential roles of lncRNA-mRNA networks in immune 
      regulation in teleost fish.
CI  - (c) 2021. The Society for In Vitro Biology.
FAU - Liu, Shuai
AU  - Liu S
AD  - School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 
      200444, China.
AD  - Research Institute of Poyang Lake, Jiangxi Academy of Sciences, Nanchang, 330012, 
      China.
FAU - Yu, Ting
AU  - Yu T
AD  - School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 
      200444, China.
FAU - Zhang, Yuanyuan
AU  - Zhang Y
AD  - School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 
      200444, China.
FAU - Pan, Chenyuan
AU  - Pan C
AD  - School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 
      200444, China.
FAU - Cai, Ling
AU  - Cai L
AD  - Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, 361005, 
      China. cailing@tio.org.cn.
FAU - Yang, Ming
AU  - Yang M
AUID- ORCID: 0000-0001-6417-4235
AD  - School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 
      200444, China. mingyang@shu.edu.cn.
LA  - eng
GR  - No.31971523/National Natural Science Foundation of China/
GR  - No.2018J05066/Natural Science Foundation of Fujian Province/
PT  - Journal Article
DEP - 20210923
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - Carps/*immunology/metabolism
MH  - Gene Expression Regulation
MH  - High-Throughput Nucleotide Sequencing
MH  - *Immunity/genetics
MH  - Macrophages/*immunology
MH  - RNA, Long Noncoding/*metabolism
MH  - RNA, Messenger/*metabolism
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - Common carp
OT  - Immune regulation
OT  - LncRNA
OT  - LncRNA-mRNA network
OT  - Primary macrophages
EDAT- 2021/09/24 06:00
MHDA- 2022/03/11 06:00
CRDT- 2021/09/23 12:29
PHST- 2021/03/16 00:00 [received]
PHST- 2021/07/28 00:00 [accepted]
PHST- 2021/09/24 06:00 [pubmed]
PHST- 2022/03/11 06:00 [medline]
PHST- 2021/09/23 12:29 [entrez]
AID - 10.1007/s11626-021-00610-5 [pii]
AID - 10.1007/s11626-021-00610-5 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2021 Sep;57(8):835-847. doi: 
      10.1007/s11626-021-00610-5. Epub 2021 Sep 23.