PMID- 34554759
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220531
IS  - 1535-3907 (Electronic)
IS  - 1535-3893 (Linking)
VI  - 20
IP  - 11
DP  - 2021 Nov 5
TI  - Matrix and Sampling Effects on Quantification of Protein Biomarkers of 
      Drug-Induced Liver Injury.
PG  - 4985-4994
LID - 10.1021/acs.jproteome.1c00478 [doi]
AB  - Macrophage colony stimulating factor 1 receptor (MCSF1R), osteopontin (OPN), 
      high-mobility group protein B1 (HMGB1), glutamate dehydrogenase (GLDH), keratin 
      18 (K18), and caspase-cleaved keratin 18 (ccK18) are considered promising 
      mechanistic biomarkers for the diagnosis of drug-induced liver injury. Here, we 
      aim to elucidate the impact of the sample matrix and handling on the 
      quantification of these emerging protein biomarkers. We investigated effects such 
      as time from collection to centrifugation during serum (+/- gel) or EDTA plasma 
      preparation on two assay platforms: immunoaffinity liquid chromatography mass 
      spectrometric assays and sandwich immunoassays. Furthermore, we measured GLDH 
      activity with an enzymatic activity assay. Matrix effects were observed 
      particularly for HMGB1 and MCSF1R. HMGB1 levels were higher in serum than in 
      plasma, whereas higher concentrations of MCSF1R were observed in plasma than in 
      serum. A comparison of sample collection to centrifugation time ranging from 15 
      to 60 min demonstrated increasing levels of HMGB1 in serum, while MCSF1R, OPN, 
      GLDH, and ccK18 concentrations remained stable. Additionally, there was a poor 
      correlation in HMGB1 and ccK18 levels between serum and plasma. Considering the 
      observed matrix effects, we recommend plasma as a matrix of choice and 
      cross-study comparison studies to be limited to those using the same matrix.
FAU - Anselm, Viktoria
AU  - Anselm V
AD  - SIGNATOPE GmbH, Reutlingen 72770, Germany.
FAU - Sommersdorf, Cornelia
AU  - Sommersdorf C
AD  - SIGNATOPE GmbH, Reutlingen 72770, Germany.
FAU - Carrasco-Triguero, Montserrat
AU  - Carrasco-Triguero M
AD  - Genentech, San Francisco, California 94080, United States.
FAU - Katavolos, Paula
AU  - Katavolos P
AD  - Genentech, San Francisco, California 94080, United States.
AD  - Bristol-Myers Squibb, New Brunswick, New Jersey 08901, United States (at 
      Genentech during the conduct of this study).
FAU - Planatscher, Hannes
AU  - Planatscher H
AD  - SIGNATOPE GmbH, Reutlingen 72770, Germany.
FAU - Steinhilber, Andreas
AU  - Steinhilber A
AD  - SIGNATOPE GmbH, Reutlingen 72770, Germany.
FAU - Joos, Thomas
AU  - Joos T
AD  - SIGNATOPE GmbH, Reutlingen 72770, Germany.
AD  - NMI Natural and Medical Sciences Institute at the University of Tuebingen, 
      Reutlingen 72770, Germany.
FAU - Poetz, Oliver
AU  - Poetz O
AUID- ORCID: 0000-0002-1189-9547
AD  - SIGNATOPE GmbH, Reutlingen 72770, Germany.
AD  - NMI Natural and Medical Sciences Institute at the University of Tuebingen, 
      Reutlingen 72770, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210923
PL  - United States
TA  - J Proteome Res
JT  - Journal of proteome research
JID - 101128775
RN  - 0 (Biomarkers)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, human)
RN  - 0 (Keratin-18)
RN  - 0 (Proteins)
RN  - 0 (SPP1 protein, human)
RN  - 106441-73-0 (Osteopontin)
RN  - EC 1.4.1.2 (Glutamate Dehydrogenase)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Biomarkers
MH  - *Chemical and Drug Induced Liver Injury/diagnosis
MH  - Glutamate Dehydrogenase
MH  - HMGB1 Protein
MH  - Humans
MH  - Keratin-18
MH  - Osteopontin
MH  - Proteins
MH  - Receptor, Macrophage Colony-Stimulating Factor
MH  - Specimen Handling
OTO - NOTNLM
OT  - centrifugation
OT  - drug-induced liver injury
OT  - immunoassay
OT  - mass spectrometry
OT  - plasma
OT  - preanalytical effects
OT  - serum
OT  - serum gel
EDAT- 2021/09/24 06:00
MHDA- 2022/02/26 06:00
CRDT- 2021/09/23 17:13
PHST- 2021/09/24 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2021/09/23 17:13 [entrez]
AID - 10.1021/acs.jproteome.1c00478 [doi]
PST - ppublish
SO  - J Proteome Res. 2021 Nov 5;20(11):4985-4994. doi: 10.1021/acs.jproteome.1c00478. 
      Epub 2021 Sep 23.