PMID- 34555172
OWN - NLM
STAT- MEDLINE
DCOM- 20211224
LR  - 20240405
IS  - 1476-5535 (Electronic)
IS  - 1367-5435 (Print)
IS  - 1367-5435 (Linking)
VI  - 48
IP  - 9-10
DP  - 2021 Dec 23
TI  - Properties of an acid-tolerant, persistent Cheddar cheese isolate, 
      Lacticaseibacillus paracasei GCRL163.
LID - 10.1093/jimb/kuab070 [doi]
LID - kuab070
AB  - The distinctive flavours in hard cheeses are attributed largely to the activity 
      of nonstarter lactic acid bacteria (NSLAB) which dominate the cheese matrix 
      during maturation after lactose is consumed. Understanding how different strains 
      of NSLAB survive, compete, and scavenge available nutrients is fundamental to 
      selecting strains as potential adjunct starters which may influence product 
      traits. Three Lacticaseibacillus paracasei isolates which dominated at different 
      stages over 63-week maturation periods of Australian Cheddar cheeses had the same 
      molecular biotype. They shared many phenotypic traits, including salt tolerance, 
      optimum growth temperature, growth on N-acetylglucosamine and 
      N-acetylgalactosamine plus delayed growth on D-ribose, carbon sources likely 
      present in cheese due to bacterial autolysis. However, strains 124 and 163 (later 
      named GCRL163) survived longer at low pH and grew on D-tagatose and D-mannitol, 
      differentiating this phenotype from strain 122. When cultured on growth-limiting 
      lactose (0.2%, wt/vol) in the presence of high concentrations of L-leucine and 
      other amino acids, GCRL163 produced, and subsequently consumed lactate, forming 
      acetic and formic acids, and demonstrated temporal accumulation of intermediates 
      in pyruvate metabolism in long-term cultures. Strain GCRL163 grew in Tween 
      80-tryptone broths, a trait not shared by all L. casei-group dairy isolates 
      screened in this study. Including citrate in this medium stimulated growth of 
      GCRL163 above citrate alone, suggesting cometabolism of citrate and Tween 80. 
      Proteomic analysis of cytosolic proteins indicated that growth in Tween 80 
      produced a higher stress state and increased relative abundance of three cell 
      envelope proteinases (CEPs) (including PrtP and Dumpy), amongst over 230 
      differentially expressed proteins.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of Society 
      of Industrial Microbiology and Biotechnology.
FAU - Shah, Syed S
AU  - Shah SS
AD  - Food Safety and Innovation Centre, Tasmanian Institute of Agriculture, University 
      of Tasmania, Hobart 7005, Australia.
FAU - Al-Naseri, Ali
AU  - Al-Naseri A
AD  - Food Safety and Innovation Centre, Tasmanian Institute of Agriculture, University 
      of Tasmania, Hobart 7005, Australia.
FAU - Rouch, Duncan
AU  - Rouch D
AD  - Clarendon Policy and Strategy Group, Melbourne 3000, Australia.
FAU - Bowman, John P
AU  - Bowman JP
AD  - Food Safety and Innovation Centre, Tasmanian Institute of Agriculture, University 
      of Tasmania, Hobart 7005, Australia.
FAU - Wilson, Richard
AU  - Wilson R
AD  - Central Science Laboratory, University of Tasmania, Hobart 7005, Australia.
FAU - Baker, Anthony L
AU  - Baker AL
AD  - Food Safety and Innovation Centre, Tasmanian Institute of Agriculture, University 
      of Tasmania, Hobart 7005, Australia.
FAU - Britz, Margaret L
AU  - Britz ML
AUID- ORCID: 0000-0002-6413-3756
AD  - Food Safety and Innovation Centre, Tasmanian Institute of Agriculture, University 
      of Tasmania, Hobart 7005, Australia.
LA  - eng
GR  - ACTP029/Dairy Australia/
GR  - University of Tasmania/
PT  - Journal Article
PL  - Germany
TA  - J Ind Microbiol Biotechnol
JT  - Journal of industrial microbiology & biotechnology
JID - 9705544
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Australia
MH  - *Cheese
MH  - Lactic Acid
MH  - *Lactobacillales/genetics
MH  - Proteomics
PMC - PMC8788758
OTO - NOTNLM
OT  - Lactobacillus
OT  - Nutrient starvation
OT  - Proteomics
OT  - Tween 80
EDAT- 2021/09/24 06:00
MHDA- 2021/12/25 06:00
PMCR- 2021/09/23
CRDT- 2021/09/23 17:27
PHST- 2021/08/13 00:00 [received]
PHST- 2021/09/11 00:00 [accepted]
PHST- 2021/09/24 06:00 [pubmed]
PHST- 2021/12/25 06:00 [medline]
PHST- 2021/09/23 17:27 [entrez]
PHST- 2021/09/23 00:00 [pmc-release]
AID - 6374559 [pii]
AID - kuab070 [pii]
AID - 10.1093/jimb/kuab070 [doi]
PST - ppublish
SO  - J Ind Microbiol Biotechnol. 2021 Dec 23;48(9-10):kuab070. doi: 
      10.1093/jimb/kuab070.