PMID- 34555778
OWN - NLM
STAT- MEDLINE
DCOM- 20220302
LR  - 20220302
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 73
DP  - 2021 Dec
TI  - Overexpressed LINC00467 promotes the viability and proliferation yet inhibits 
      apoptosis of gastric cancer cells via raising ITGB3 level.
PG  - 101644
LID - S0040-8166(21)00160-9 [pii]
LID - 10.1016/j.tice.2021.101644 [doi]
AB  - Long non-coding RNA (lncRNA) LINC00467 plays a proto-oncogenic role in non-small 
      cell lung cancer. However, its effect and modulatory mechanism in gastric cancer 
      (GC) are unknown. Thereby, we elucidated the mechanism of LINC00467 in GC. 
      LINC00467 level in GC tissues was assessed by bioinformatic analysis, and 
      clinicopathological parameters from GC patients were collected. The levels of 
      LINC00467, integrin subunit beta 3 (ITGB3), proliferating cell nuclear antigen 
      (PCNA), cleaved caspase-3 and cleaved poly (ADP-ribose) polymerase 1 (PARP1) in 
      tissue samples or treated GC cells were assessed by quantitative real-time 
      polymerase chain reaction (qRT-PCR), fluorescence in situ hybridization (FISH), 
      or Western blot. The viability, proliferation and apoptosis of GC cells were 
      detected by methyl thiazolyl tetrazolium assay, colony formation assay, and flow 
      cytometry. Levels of LINC00467 and ITGB3 were up-regulated in GC, and highly 
      expressed LINC00467 was positively associated with tumor size, differentiation, N 
      stage, and T stage in GC patients. LINC00467 was enriched in cytoplasm of GC 
      cells, and overexpressed LINC00467 promoted the viability and proliferation as 
      well as levels of ITGB3 and PCNA, while suppressing the apoptosis and levels of 
      cleaved caspase-3 and cleaved PARP1 in GC cells. Besides, the effects of 
      shLINC00467 on inhibiting cell viability, proliferation of GC cells and PCNA 
      level and promoting apoptosis as well as levels of cleaved caspase-3 and cleaved 
      PARP1 were all partially reversed by overexpressed ITGB3. Overexpressed LINC00467 
      enhanced the viability and proliferation but inhibited apoptosis of GC cells via 
      increasing ITGB3 level.
CI  - Copyright (c) 2021. Published by Elsevier Ltd.
FAU - Xu, Limao
AU  - Xu L
AD  - Gastroenterology Department, The 3(rd) Affiliated Hospital of Chengdu Medical 
      College, Pidu District People's Hospital, Chengdu, China.
FAU - Liu, Chengmin
AU  - Liu C
AD  - Gastroenterology Department, The 3(rd) Affiliated Hospital of Chengdu Medical 
      College, Pidu District People's Hospital, Chengdu, China.
FAU - Ye, Zhiyao
AU  - Ye Z
AD  - Gastroenterology Department, The 3(rd) Affiliated Hospital of Chengdu Medical 
      College, Pidu District People's Hospital, Chengdu, China.
FAU - Wu, Chengfeng
AU  - Wu C
AD  - Gastroenterology Department, The 3(rd) Affiliated Hospital of Chengdu Medical 
      College, Pidu District People's Hospital, Chengdu, China.
FAU - Ding, Yuhang
AU  - Ding Y
AD  - Gastroenterology Department, The 3(rd) Affiliated Hospital of Chengdu Medical 
      College, Pidu District People's Hospital, Chengdu, China.
FAU - Huang, Juan
AU  - Huang J
AD  - Gastroenterology Department, The 3(rd) Affiliated Hospital of Chengdu Medical 
      College, Pidu District People's Hospital, Chengdu, China. Electronic address: 
      huangjun_hj1@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210921
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Integrin beta3)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Long Noncoding)
RN  - EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Aged
MH  - Apoptosis/*genetics
MH  - Caspase 3/metabolism
MH  - Cell Differentiation/genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Cell Survival/genetics
MH  - Cytoplasm/metabolism
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Silencing
MH  - Humans
MH  - Integrin beta3/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Poly(ADP-ribose) Polymerases/metabolism
MH  - Proliferating Cell Nuclear Antigen/metabolism
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Stomach Neoplasms/*genetics/*pathology
MH  - Tumor Burden
MH  - Up-Regulation/genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Gastric cancer
OT  - ITGB3
OT  - LINC00467
OT  - Proliferation
EDAT- 2021/09/24 06:00
MHDA- 2022/03/03 06:00
CRDT- 2021/09/23 20:29
PHST- 2021/04/26 00:00 [received]
PHST- 2021/08/24 00:00 [revised]
PHST- 2021/09/04 00:00 [accepted]
PHST- 2021/09/24 06:00 [pubmed]
PHST- 2022/03/03 06:00 [medline]
PHST- 2021/09/23 20:29 [entrez]
AID - S0040-8166(21)00160-9 [pii]
AID - 10.1016/j.tice.2021.101644 [doi]
PST - ppublish
SO  - Tissue Cell. 2021 Dec;73:101644. doi: 10.1016/j.tice.2021.101644. Epub 2021 Sep 
      21.