PMID- 34557501
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210925
IS  - 2296-858X (Print)
IS  - 2296-858X (Electronic)
IS  - 2296-858X (Linking)
VI  - 8
DP  - 2021
TI  - Glucocorticoid Effects on Tissue Residing Immune Cells in Giant Cell Arteritis: 
      Importance of GM-CSF.
PG  - 709404
LID - 10.3389/fmed.2021.709404 [doi]
LID - 709404
AB  - Giant cell arteritis (GCA) is a systemic granulomatous vasculitis clinically 
      characterized by a prompt response to glucocorticoid therapy. Dendritic cells 
      (DCs) play a central role in the pathogenesis of the disease and are increased in 
      temporal arteries from GCA patients. The aim of this study was to determine the 
      effects of glucocorticoid therapy on granulomatous infiltrates and on peripheral 
      DCs of GCA patients. Immunohistochemical staining of temporal artery specimens 
      from 41 GCA patients revealed a rapid reduction of the number of DCs after 
      initiation of glucocorticoid treatment. TUNEL staining was performed to quantify 
      apoptotic S100+ DC, CD3+ T cells, and CD68+ macrophages in the granulomatous 
      infiltrates. An increase of apoptotic cells up to 9 +/- 2% after 4-5 days of 
      glucocorticoid therapy and up to 27 +/- 5% (p < 0.001, compared to earlier 
      timepoints) after 6-10 days was detected. A decrease of CCL19 and CCL21 
      expression was observed after starting glucocorticoid therapy. 
      Granulocyte-macrophage colony-stimulating factor (GM-CSF) expression also 
      significantly decreased under glucocorticoid therapy. No GM-CSF expression was 
      detected in the control specimens. Glucocorticoid therapy leads to a rapid, 
      time-dependent reduction of DCs in temporal arteries from GCA patients and 
      reduction of mediators for cell migration. Our data suggest GM-CSF as a novel 
      therapeutic target of GCA.
CI  - Copyright (c) 2021 Wagner, Wittkop, Thalmann, Willmen, Godecke, Hodam, Ronicke and 
      Zenke.
FAU - Wagner, Annette D
AU  - Wagner AD
AD  - Department of Nephrology and Hypertension, Hannover Medical School, Hannover, 
      Germany.
FAU - Wittkop, Ulrike
AU  - Wittkop U
AD  - Department of Nephrology and Hypertension, Hannover Medical School, Hannover, 
      Germany.
FAU - Thalmann, Jessica
AU  - Thalmann J
AD  - Department of Nephrology and Hypertension, Hannover Medical School, Hannover, 
      Germany.
FAU - Willmen, Tina
AU  - Willmen T
AD  - Department of Nephrology and Hypertension, Hannover Medical School, Hannover, 
      Germany.
FAU - Godecke, Vega
AU  - Godecke V
AD  - Department of Nephrology and Hypertension, Hannover Medical School, Hannover, 
      Germany.
FAU - Hodam, Justyna
AU  - Hodam J
AD  - Deutsches Rotes Kreuz (DRK) Clinic for Child and Adolescent Psychiatry, Bad 
      Neuenahr, Germany.
FAU - Ronicke, Simon
AU  - Ronicke S
AD  - Department of Nephrology and Hypertension, Hannover Medical School, Hannover, 
      Germany.
FAU - Zenke, Martin
AU  - Zenke M
AD  - Department of Cell Biology, Institute for Biomedical Engineering, 
      Rheinisch-Westfalische Technische Hochschule Aachen (RWTH) Aachen University 
      Medical School, Aachen, Germany.
AD  - Helmholtz Institute for Biomedical Engineering, Rheinisch-Westfalische Technische 
      Hochschule Aachen (RWTH) Aachen University, Aachen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20210907
PL  - Switzerland
TA  - Front Med (Lausanne)
JT  - Frontiers in medicine
JID - 101648047
PMC - PMC8452956
OTO - NOTNLM
OT  - GM-CSF
OT  - apoptosis
OT  - dendritic cells
OT  - giant cell arteritis
OT  - glucocorticoids
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/09/25 06:00
MHDA- 2021/09/25 06:01
PMCR- 2021/09/07
CRDT- 2021/09/24 07:10
PHST- 2021/05/13 00:00 [received]
PHST- 2021/08/17 00:00 [accepted]
PHST- 2021/09/24 07:10 [entrez]
PHST- 2021/09/25 06:00 [pubmed]
PHST- 2021/09/25 06:01 [medline]
PHST- 2021/09/07 00:00 [pmc-release]
AID - 10.3389/fmed.2021.709404 [doi]
PST - epublish
SO  - Front Med (Lausanne). 2021 Sep 7;8:709404. doi: 10.3389/fmed.2021.709404. 
      eCollection 2021.