PMID- 34557530 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210925 IS - 2297-055X (Print) IS - 2297-055X (Electronic) IS - 2297-055X (Linking) VI - 8 DP - 2021 TI - Expression and Functional Analysis of lncRNAs Involved in Platelet-Derived Growth Factor-BB-Induced Proliferation of Human Aortic Smooth Muscle Cells. PG - 702718 LID - 10.3389/fcvm.2021.702718 [doi] LID - 702718 AB - Abnormal proliferation of vascular smooth muscle cells (VSMCs) is a common feature of many vascular remodeling diseases. Because long non-coding RNAs (lncRNAs) play a critical role in cardiovascular diseases, we analyzed the key lncRNAs that regulate VSMC proliferation. Microarray analysis identified 2,643 differentially expressed lncRNAs (DELs) and 3,720 differentially expressed coding genes (DEGs) between fetal bovine serum (FBS) starvation-induced quiescent human aortic smooth muscle cells (HASMCs) and platelet-derived growth factor-BB (PDGF-BB)-stimulated proliferative HASMCs. Gene Ontology and pathway analyses of the identified DEGs and DELs demonstrated that many lncRNAs were enriched in pathways related to cell proliferation. One of the upregulated lncRNAs in proliferative HASMC was HIF1A anti-sense RNA 2 (HIF1A-AS2). HIF1A-AS2 suppression decreased HASMC proliferation via the miR-30e-5p/CCND2 mRNA axis. We have thus identified key DELs and DEGs involved in the regulation of PDGF-BB induced HASMC proliferation. Moreover, HIF1A-AS2 promotes HASMC proliferation, suggesting its potential involvement in VSMC proliferative vascular diseases. CI - Copyright (c) 2021 Lin, Chen, Gong, Xu, Du, Wang, Yang, Wang, Liu, Kong, Li, Liu, Li, Dong and Sun. FAU - Lin, Jia-Jie AU - Lin JJ AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Chen, Wei AU - Chen W AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. AD - Stem Cell Translational Research Center, Tongji Hospital, Tongji University School of Medicine, Shanghai, China. FAU - Gong, Miao AU - Gong M AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Xu, Xin AU - Xu X AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Du, Mei-Yang AU - Du MY AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Wang, Si-Fan AU - Wang SF AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Yang, Li-Yun AU - Yang LY AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Wang, Yu AU - Wang Y AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Liu, Ke-Xin AU - Liu KX AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Kong, Peng AU - Kong P AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Li, Bin AU - Li B AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Liu, Kun AU - Liu K AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Li, Yi-Ming AU - Li YM AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Dong, Li-Hua AU - Dong LH AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. FAU - Sun, Shao-Guang AU - Sun SG AD - Department of Biochemistry and Molecular Biology, Key Laboratory of Medical Biotechnology of Hebei Province, Cardiovascular Medical Science Center, Hebei Medical University, Shijiazhuang, China. LA - eng PT - Journal Article DEP - 20210907 PL - Switzerland TA - Front Cardiovasc Med JT - Frontiers in cardiovascular medicine JID - 101653388 PMC - PMC8452921 OTO - NOTNLM OT - HIF1A-AS2 OT - PDGF OT - human aortic smooth muscle cell OT - long non-coding RNA OT - proliferation COIS- The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. EDAT- 2021/09/25 06:00 MHDA- 2021/09/25 06:01 PMCR- 2021/01/01 CRDT- 2021/09/24 07:10 PHST- 2021/04/29 00:00 [received] PHST- 2021/08/16 00:00 [accepted] PHST- 2021/09/24 07:10 [entrez] PHST- 2021/09/25 06:00 [pubmed] PHST- 2021/09/25 06:01 [medline] PHST- 2021/01/01 00:00 [pmc-release] AID - 10.3389/fcvm.2021.702718 [doi] PST - epublish SO - Front Cardiovasc Med. 2021 Sep 7;8:702718. doi: 10.3389/fcvm.2021.702718. eCollection 2021.