PMID- 34561969
OWN - NLM
STAT- MEDLINE
DCOM- 20220127
LR  - 20220531
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
VI  - 22
IP  - 1-2
DP  - 2022 Jan
TI  - Characterization of HLA-A*33:03 epitopes via immunoprecipitation and LC-MS/MS.
PG  - e2100171
LID - 10.1002/pmic.202100171 [doi]
AB  - Human leukocyte antigen (HLA) class I has more than 18,000 alleles, each of which 
      binds to a set of unique peptides from the cellular degradome. Deciphering the 
      interaction between antigenic peptides and HLA proteins is crucial for 
      understanding immune responses in autoimmune diseases and cancer. In this study, 
      we aimed to characterize the peptidome that binds to HLA-A*33:03, which is one of 
      the most prevalent HLA-A alleles in the Northeast Asian population, but poorly 
      studied. For this purpose, we analyzed the HLA-A*33:03 monoallelic B cell line 
      using immunoprecipitation of HLA-A and peptide complexes, followed by liquid 
      chromatography-tandem mass spectrometry (LC-MS/MS). In this study, we identified 
      5731 unique peptides that were associated with HLA A*33:03, and experimentally 
      validated the affinity of 40 peptides for HLA-A*33:03 and their stability in HLA 
      A*33:03-peptides complexes. To our knowledge, this study represents the largest 
      dataset of peptides associated with HLA-A*33:03. Also, this is the first study in 
      which HLA A*33:03-associated peptides were experimentally validated.
CI  - (c) 2021 Wiley-VCH GmbH.
FAU - Khan, Amir
AU  - Khan A
AD  - Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, 
      Republic of Korea.
AD  - Department of Genome Medicine and Science, College of Medicine, Gachon 
      University, Incheon, Republic of Korea.
FAU - Shin, Ji-Yon
AU  - Shin JY
AD  - Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, 
      Republic of Korea.
FAU - So, Min Kyung
AU  - So MK
AD  - New Drug Development Center, Osong Medical Innovation Foundation, Cheongju-si, 
      Republic of Korea.
FAU - Na, Jung-Hyun
AU  - Na JH
AUID- ORCID: 0000-0002-5257-2428
AD  - Department of Pharmaceutical Engineering, Sangji University, Wonju, Republic of 
      Korea.
FAU - Justesen, Sune
AU  - Justesen S
AD  - Immunitrack, Copenhagen, Denmark.
FAU - Ansari, Adnan Ahmad
AU  - Ansari AA
AD  - National Center for Bioinformatics, Quaid-i-Azam University, Islamabad, Pakistan.
FAU - Ko, Byoung Joon
AU  - Ko BJ
AD  - School of Biopharmaceutical and Medical Sciences, Sungshin Women's University, 
      Seoul, Republic of Korea.
FAU - Ahn, Sung-Min
AU  - Ahn SM
AUID- ORCID: 0000-0002-5031-1929
AD  - Department of Health Sciences and Technology, GAIHST, Gachon University, Incheon, 
      Republic of Korea.
AD  - Department of Genome Medicine and Science, College of Medicine, Gachon 
      University, Incheon, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211005
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Epitopes)
RN  - 0 (HLA-A Antigens)
SB  - IM
MH  - Chromatography, Liquid
MH  - Epitopes
MH  - *HLA-A Antigens
MH  - Humans
MH  - Immunoprecipitation
MH  - *Tandem Mass Spectrometry
OTO - NOTNLM
OT  - HLA-A*33:03
OT  - cancer vaccine
OT  - epitope
OT  - human leukocyte antigen
OT  - mass spectrometry
EDAT- 2021/09/26 06:00
MHDA- 2022/01/28 06:00
CRDT- 2021/09/25 06:31
PHST- 2021/08/29 00:00 [revised]
PHST- 2021/07/22 00:00 [received]
PHST- 2021/09/22 00:00 [accepted]
PHST- 2021/09/26 06:00 [pubmed]
PHST- 2022/01/28 06:00 [medline]
PHST- 2021/09/25 06:31 [entrez]
AID - 10.1002/pmic.202100171 [doi]
PST - ppublish
SO  - Proteomics. 2022 Jan;22(1-2):e2100171. doi: 10.1002/pmic.202100171. Epub 2021 Oct 
      5.