PMID- 34562815
OWN - NLM
STAT- MEDLINE
DCOM- 20211101
LR  - 20211101
IS  - 1878-4291 (Electronic)
IS  - 0968-4328 (Linking)
VI  - 151
DP  - 2021 Dec
TI  - High-resolution imaging and fast number estimation of suspended particles using 
      dewetted polymer microlenses in a microfluidic channel.
PG  - 103148
LID - S0968-4328(21)00139-6 [pii]
LID - 10.1016/j.micron.2021.103148 [doi]
AB  - We have fabricated polymer micro-lens array by self-organized dewetting inside 
      the microchannel, which shows remarkable enhancement in the resolution, contrast 
      and more than 10 times add-on magnification to a microscope. These lenses are 
      demonstrated to resolve sub-micrometer features and detect moving micro-particles 
      when suspension is flown in a microchannel. Polystyrene (PS) micro-lenses are 
      fabricated on a polydimethylsiloxane (PDMS) substrate using the controlled 
      dewetting of PS thin film then this PDMS substrate is used to close the 
      microchannel with inverted micro-lenses on it. An aqueous suspension of 
      polystyrene particles is flown through the microchannel and we have observed the 
      particles through an optical microscope. Focusing and magnification through PS 
      micro-lenses is analyzed to get a quantitative estimate of the particle number 
      density in the solution. This method offers a promising low-cost high throughput 
      solution for determining the approximate number density of flowing particles or 
      suitably stained biological cells. Particularly in a pathology lab it can 
      tremendously increase detection limit by enabling visibility of sub-micrometer 
      pathogens using a standard laboratory microscope.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Mishra, Shubham
AU  - Mishra S
AD  - Department of Chemical Engineering and Technology, Indian Institute of Technology 
      (Banaras Hindu University), Varanasi, UP, 221005, India.
FAU - Kulkarni, Manish M
AU  - Kulkarni MM
AD  - Center for Nanosciences, Indian Institute of Technology Kanpur, Kanpur, UP, 
      208016, India.
FAU - Verma, Ankur
AU  - Verma A
AD  - Department of Chemical Engineering and Technology, Indian Institute of Technology 
      (Banaras Hindu University), Varanasi, UP, 221005, India. Electronic address: 
      ankurv.che@iitbhu.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20210916
PL  - England
TA  - Micron
JT  - Micron (Oxford, England : 1993)
JID - 9312850
RN  - 0 (Polymers)
RN  - 0 (Polystyrenes)
SB  - IM
MH  - *Lenses
MH  - *Microfluidics
MH  - Microscopy
MH  - Polymers
MH  - Polystyrenes
OTO - NOTNLM
OT  - Cytometer
OT  - Dewetting
OT  - Microchannel
OT  - Microlens
OT  - Polymer thin-film
EDAT- 2021/09/26 06:00
MHDA- 2021/11/03 06:00
CRDT- 2021/09/25 20:24
PHST- 2021/06/09 00:00 [received]
PHST- 2021/09/13 00:00 [revised]
PHST- 2021/09/14 00:00 [accepted]
PHST- 2021/09/26 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2021/09/25 20:24 [entrez]
AID - S0968-4328(21)00139-6 [pii]
AID - 10.1016/j.micron.2021.103148 [doi]
PST - ppublish
SO  - Micron. 2021 Dec;151:103148. doi: 10.1016/j.micron.2021.103148. Epub 2021 Sep 16.