PMID- 34562919
OWN - NLM
STAT- MEDLINE
DCOM- 20211104
LR  - 20211104
IS  - 2079-6374 (Electronic)
IS  - 2079-6374 (Linking)
VI  - 11
IP  - 9
DP  - 2021 Sep 10
TI  - Low-Power Two-Color Stimulated Emission Depletion Microscopy for Live Cell 
      Imaging.
LID - 10.3390/bios11090330 [doi]
LID - 330
AB  - Stimulated emission depletion (STED) microscopy is a typical laser-scanning 
      super-resolution imaging technology, the emergence of which has opened a new 
      research window for studying the dynamic processes of live biological samples on 
      a nanometer scale. According to the characteristics of STED, a high depletion 
      power is required to obtain a high resolution. However, a high laser power can 
      induce severe phototoxicity and photobleaching, which limits the applications for 
      live cell imaging, especially in two-color STED super-resolution imaging. 
      Therefore, we developed a low-power two-color STED super-resolution microscope 
      with a single supercontinuum white-light laser. Using this system, we achieved 
      low-power two-color super-resolution imaging based on digital enhancement 
      technology. Lateral resolutions of 109 and 78 nm were obtained for mitochondria 
      and microtubules in live cells, respectively, with 0.8 mW depletion power. These 
      results highlight the great potential of the novel digitally enhanced two-color 
      STED microscopy for long-term dynamic imaging of live cells.
FAU - Zhang, Jia
AU  - Zhang J
AD  - Key Laboratory of Optoelectronic Devices and System of Ministry of Education and 
      Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen 
      University, Shenzhen 518060, China.
FAU - Gao, Xinwei
AU  - Gao X
AD  - Key Laboratory of Optoelectronic Devices and System of Ministry of Education and 
      Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen 
      University, Shenzhen 518060, China.
FAU - Wang, Luwei
AU  - Wang L
AD  - Key Laboratory of Optoelectronic Devices and System of Ministry of Education and 
      Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen 
      University, Shenzhen 518060, China.
FAU - Guo, Yong
AU  - Guo Y
AD  - Key Laboratory of Optoelectronic Devices and System of Ministry of Education and 
      Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen 
      University, Shenzhen 518060, China.
FAU - Zhu, Yinru
AU  - Zhu Y
AD  - Key Laboratory of Optoelectronic Devices and System of Ministry of Education and 
      Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen 
      University, Shenzhen 518060, China.
FAU - Yang, Zhigang
AU  - Yang Z
AD  - Key Laboratory of Optoelectronic Devices and System of Ministry of Education and 
      Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen 
      University, Shenzhen 518060, China.
FAU - Yan, Wei
AU  - Yan W
AUID- ORCID: 0000-0002-0745-9514
AD  - Key Laboratory of Optoelectronic Devices and System of Ministry of Education and 
      Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen 
      University, Shenzhen 518060, China.
FAU - Qu, Junle
AU  - Qu J
AUID- ORCID: 0000-0001-7833-4711
AD  - Key Laboratory of Optoelectronic Devices and System of Ministry of Education and 
      Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen 
      University, Shenzhen 518060, China.
LA  - eng
GR  - 61620106016, 61835009, 62005171 and 61975127/National Natural Science Foundation 
      of China/
GR  - 2017YFA0700500/the National Basic Research Program of China/
PT  - Journal Article
DEP - 20210910
PL  - Switzerland
TA  - Biosensors (Basel)
JT  - Biosensors
JID - 101609191
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Fluorescent Dyes
MH  - Lasers
MH  - *Microscopy
MH  - Microscopy, Fluorescence
PMC - PMC8468006
OTO - NOTNLM
OT  - dynamic live cell imaging
OT  - low power STED
OT  - stimulated emission depletion
OT  - super-resolution microscopy
COIS- The authors declare that there are no conflict of interest related to this 
      article.
EDAT- 2021/09/26 06:00
MHDA- 2021/11/05 06:00
PMCR- 2021/09/10
CRDT- 2021/09/25 20:27
PHST- 2021/08/03 00:00 [received]
PHST- 2021/08/27 00:00 [revised]
PHST- 2021/09/09 00:00 [accepted]
PHST- 2021/09/25 20:27 [entrez]
PHST- 2021/09/26 06:00 [pubmed]
PHST- 2021/11/05 06:00 [medline]
PHST- 2021/09/10 00:00 [pmc-release]
AID - bios11090330 [pii]
AID - biosensors-11-00330 [pii]
AID - 10.3390/bios11090330 [doi]
PST - epublish
SO  - Biosensors (Basel). 2021 Sep 10;11(9):330. doi: 10.3390/bios11090330.