PMID- 34563036
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211002
IS  - 2296-3529 (Print)
IS  - 2296-3529 (Electronic)
IS  - 2296-3529 (Linking)
VI  - 8
IP  - 3
DP  - 2021 Sep 1
TI  - Immunohistopathological Analysis of Immunoglobulin E-Positive Epidermal Dendritic 
      Cells with House Dust Mite Antigens in Naturally Occurring Skin Lesions of Adult 
      and Elderly Patients with Atopic Dermatitis.
PG  - 426-441
LID - 10.3390/dermatopathology8030045 [doi]
AB  - The immunopathogenic role of house dust mite (HDM) allergens in the development 
      of skin lesions in atopic dermatitis (AD) has not yet been precisely clarified. 
      We immunohistopathologically evaluated the localization of immunoglobulin E 
      (IgE)-positive epidermal dendritic cells with HDM antigens in the skin lesions of 
      patients with IgE-allergic AD. Using double-immunofluorescence and 
      single-immunochemical staining methods, we analyzed biopsy specimens from the 
      skin lesions of six patients with IgE-allergic AD and HDM allergy and 11 control 
      subjects with inflammatory skin disorders. Inflammatory dendritic epidermal cells 
      (IDECs; CD11c+ and CD206+ cells) were markedly observed in the central area of 
      the spongiotic epidermis of skin lesions in all AD patients. Furthermore, 
      IgE-positive IDECs with HDM antigens in the central areas of the spongiosis were 
      found in four of the six (66.7%) AD patients. Langerhans cells (LCs; CD207+ 
      cells) with HDM antigens were also observed in the peripheral areas of the 
      spongiosis. Infiltration of CD4+ and CD8+ T cells in association with 
      IgE-positive IDECs and LCs with HDM antigens was seen in the spongiotic 
      epidermis. An IgE-mediated delayed-type hypersensitivity reaction, in combination 
      with IgE-bearing dendritic cells, specific T cells, keratinocytes, and HDM 
      antigens, may lead to spongiotic tissue formation in eczematous dermatitis in AD.
FAU - Tanei, Ryoji
AU  - Tanei R
AUID- ORCID: 0000-0003-3774-2561
AD  - Department of Dermatology, Tokyo Metropolitan Geriatric Hospital and Institute of 
      Gerontology, Itabashi, Tokyo 173-0015, Japan.
FAU - Hasegawa, Yasuko
AU  - Hasegawa Y
AD  - Department of Geriatric Pathology, Tokyo Metropolitan Geriatric Hospital and 
      Institute of Gerontology, Itabashi, Tokyo 173-0015, Japan.
LA  - eng
PT  - Journal Article
DEP - 20210901
PL  - Switzerland
TA  - Dermatopathology (Basel)
JT  - Dermatopathology (Basel, Switzerland)
JID - 101651125
PMC - PMC8482222
OTO - NOTNLM
OT  - Langerhans cells
OT  - double-immunofluorescence staining
OT  - house dust mite antigens
OT  - immunohistopathology
OT  - inflammatory dendritic epidermal cells
OT  - spongiosis
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/26 06:00
MHDA- 2021/09/26 06:01
PMCR- 2021/09/01
CRDT- 2021/09/25 20:28
PHST- 2021/07/31 00:00 [received]
PHST- 2021/08/09 00:00 [revised]
PHST- 2021/08/12 00:00 [accepted]
PHST- 2021/09/25 20:28 [entrez]
PHST- 2021/09/26 06:00 [pubmed]
PHST- 2021/09/26 06:01 [medline]
PHST- 2021/09/01 00:00 [pmc-release]
AID - dermatopathology8030045 [pii]
AID - dermatopathology-08-00045 [pii]
AID - 10.3390/dermatopathology8030045 [doi]
PST - epublish
SO  - Dermatopathology (Basel). 2021 Sep 1;8(3):426-441. doi: 
      10.3390/dermatopathology8030045.