PMID- 34563784
OWN - NLM
STAT- MEDLINE
DCOM- 20211126
LR  - 20211126
IS  - 1879-1298 (Electronic)
IS  - 0045-6535 (Linking)
VI  - 287
IP  - Pt 3
DP  - 2022 Jan
TI  - Application of 96-well plate SPE method for analysis of persistent organic 
      pollutants in low volume blood serum samples.
PG  - 132300
LID - S0045-6535(21)02772-7 [pii]
LID - 10.1016/j.chemosphere.2021.132300 [doi]
AB  - Though many persistent organic pollutants (POPs) are closely regulated the human 
      population is still exposed to these ubiquitous chemicals from the environment 
      and diet. Safe management and human biomonitoring of POPs is necessary to 
      understand the risk of exposure. Within human biomonitoring the mass of sample is 
      often limited, therefore robust methods using smaller sample amounts are 
      necessary. This study developed a 96-well plate solid phase extraction (SPE) 
      method for determination of selected POPs: polychlorinated biphenyls (PCBs), 
      organochlorine pesticides (OCPs), polybrominated diphenyl ethers (PBDEs), 
      hexabromocyclododecane (HBCD) and non-persistent novel flame retardants (NFRs) in 
      low volume blood serum. Non-destructive clean-up coupling Oasis HLB extraction 
      plate with Phree phospholipid removal plate was employed. Extraction efficiency 
      was determined at low and high concentrations in certified reference materials 
      NIST SRM 1957 and 1958, respectively. Target compounds deviated from certified 
      values on average by 15% and 21% for SRM 1957 and SRM 1958, respectively. 
      Observed limit of detections (LODs) ranged from 0.36 pg/mL (PCB 180) to 
      66.07 pg/mL (delta-HCH). The applicability for real samples is demonstrated on 48 
      samples from pregnant women enrolled in the pilot phase of the CELSPAC: TNG 
      study. In total, 30 target compounds were detected in at least one sample. The 
      method developed here provides a fast and reliable analysis of human blood serum 
      with possibility to introduce automation for the sample preparation procedure.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Palat, Jiri
AU  - Palat J
AD  - RECETOX Centre, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
FAU - Kukucka, Petr
AU  - Kukucka P
AD  - RECETOX Centre, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic. 
      Electronic address: kukucka@recetox.muni.cz.
FAU - Codling, Garry P
AU  - Codling GP
AD  - RECETOX Centre, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic; 
      Toxicology Centre, University of Saskatchewan, 44 Campus Dr, SK, S7N 5B3, 
      Saskatoon, Canada.
FAU - Price, Elliott J
AU  - Price EJ
AD  - RECETOX Centre, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
FAU - Janku, Petr
AU  - Janku P
AD  - Department of Gynecology and Obstetrics, University Hospital Brno and Faculty of 
      Medicine, Masaryk University Brno, Czech Republic; Department of Nursing and 
      Midwifery, Faculty of Medicine, Masaryk University Brno, Czech Republic.
FAU - Klanova, Jana
AU  - Klanova J
AD  - RECETOX Centre, Masaryk University, Kamenice 753/5, 62500, Brno, Czech Republic.
LA  - eng
PT  - Journal Article
DEP - 20210920
PL  - England
TA  - Chemosphere
JT  - Chemosphere
JID - 0320657
RN  - 0 (Environmental Pollutants)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - 0 (Hydrocarbons, Chlorinated)
RN  - 0 (Pesticides)
RN  - DFC2HB4I0K (Polychlorinated Biphenyls)
SB  - IM
MH  - *Environmental Pollutants
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry
MH  - Halogenated Diphenyl Ethers/analysis
MH  - Humans
MH  - *Hydrocarbons, Chlorinated/analysis
MH  - Persistent Organic Pollutants
MH  - *Pesticides/analysis
MH  - *Polychlorinated Biphenyls/analysis
MH  - Pregnancy
MH  - Serum/chemistry
MH  - Solid Phase Extraction
OTO - NOTNLM
OT  - Blood serum
OT  - Chromatography-mass spectrometry
OT  - NFRs
OT  - POPs
OT  - Well-plate SPE
EDAT- 2021/09/27 06:00
MHDA- 2021/11/27 06:00
CRDT- 2021/09/26 20:45
PHST- 2021/06/28 00:00 [received]
PHST- 2021/09/06 00:00 [revised]
PHST- 2021/09/17 00:00 [accepted]
PHST- 2021/09/27 06:00 [pubmed]
PHST- 2021/11/27 06:00 [medline]
PHST- 2021/09/26 20:45 [entrez]
AID - S0045-6535(21)02772-7 [pii]
AID - 10.1016/j.chemosphere.2021.132300 [doi]
PST - ppublish
SO  - Chemosphere. 2022 Jan;287(Pt 3):132300. doi: 10.1016/j.chemosphere.2021.132300. 
      Epub 2021 Sep 20.