PMID- 34563954
OWN - NLM
STAT- MEDLINE
DCOM- 20220124
LR  - 20220124
IS  - 1950-6007 (Electronic)
IS  - 0753-3322 (Linking)
VI  - 143
DP  - 2021 Nov
TI  - Transcriptome analysis to reveal the mechanism of the effect of Echinops 
      latifolius polysaccharide B on palmitate-induced insulin-resistant.
PG  - 112203
LID - S0753-3322(21)00987-2 [pii]
LID - 10.1016/j.biopha.2021.112203 [doi]
AB  - Hepatic insulin resistance is a crucial pathological process in type 2 diabetes 
      mellitus (T2DM) associated with visceral adiposity and metabolic disorders. 
      Echinops latifolius polysaccharide B (ETPB), a polysaccharide extracted from 
      Echinops latifolius Tausch, increases insulin sensitivity in the high-fat 
      diet-fed and STZ induced SD rat model and even prevented hepatic metabolic 
      disorders. However, the mechanism by which ETPB improves carbohydrate and lipid 
      metabolisms in the liver with insulin resistance remains largely unknown. In the 
      present work, an lnsulin resistance cell model (IR-HepG2) was established. 
      Glucose consumption, glycogen content, triglycerides (TG), and free fatty acids 
      (FFAs) levels were detected. The result revealed that the intervention of ETPB 
      significantly increased glucose consumption and glycogen synthesis and reduced 
      FFAs and TG production in IR-HepG2 cells. Further, we also employed RNA-seq to 
      identify differentially expressed miRNAs (DEMs) and mRNAs (DEGs) with a fold 
      change of >/= 1.5 and p-value of <0.05. Finally, we identified 1028, 682, 382, 
      1614, 519 and 825 DEGs, and 71, 113, 94, 68, 52 and 38 DEMs in different 
      comparisons, respectively. Based on a short time-series expression miner (STEM) 
      analysis, six profiles were chosen for further analysis. Seventeen insulin 
      resistance-associated dynamic DEGs were identified during ETPB stimulation. Based 
      on these dynamic DEGs, the related miRNAs were acquired from DEMs, and an 
      integrated miRNA-mRNA regulatory network was subsequently constructed. Besides, 
      some DEGs and DEMs were validated using qPCR. This study provides transcriptomic 
      evidence of the molecular mechanism involved in HepG2 insulin resistance, leading 
      to the discovery of miRNA-based target therapies for ETPB.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Masson SAS.. All rights 
      reserved.
FAU - Li, Ran
AU  - Li R
AD  - College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 
      Inner Mongolia, PR China.
FAU - Xia, Yajuan
AU  - Xia Y
AD  - Inner Mongolia Comprehensive Center for Disease Control and Prevention, Hohhot, 
      Inner Mongolia, PR China.
FAU - Gao, Zhenzhen
AU  - Gao Z
AD  - College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 
      Inner Mongolia, PR China.
FAU - Song, Yue
AU  - Song Y
AD  - College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 
      Inner Mongolia, PR China.
FAU - Guo, Zhiwei
AU  - Guo Z
AD  - Inner Mongolia Comprehensive Center for Disease Control and Prevention, Hohhot, 
      Inner Mongolia, PR China.
FAU - Yang, Ying
AU  - Yang Y
AD  - College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, 
      Inner Mongolia, PR China. Electronic address: yangyingzsy@126.com.
LA  - eng
PT  - Journal Article
DEP - 20210924
PL  - France
TA  - Biomed Pharmacother
JT  - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
JID - 8213295
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (MicroRNAs)
RN  - 0 (Palmitates)
RN  - 0 (Plant Extracts)
RN  - 0 (Polysaccharides)
SB  - IM
MH  - *Echinops Plant/chemistry
MH  - Energy Metabolism/drug effects
MH  - *Gene Expression Profiling
MH  - Hep G2 Cells
MH  - Hepatocytes/*drug effects/metabolism
MH  - Humans
MH  - Hypoglycemic Agents/isolation & purification/*pharmacology
MH  - *Insulin Resistance/genetics
MH  - MicroRNAs/genetics/metabolism
MH  - Palmitates/*toxicity
MH  - Plant Extracts/isolation & purification/*pharmacology
MH  - Polysaccharides/isolation & purification/*pharmacology
MH  - RNA-Seq
MH  - *Transcriptome
OTO - NOTNLM
OT  - ETPB
OT  - HepG2
OT  - Insulin resistance
OT  - MiRNAs
OT  - RNA sequencing
EDAT- 2021/09/27 06:00
MHDA- 2022/01/27 06:00
CRDT- 2021/09/26 20:54
PHST- 2021/07/29 00:00 [received]
PHST- 2021/09/08 00:00 [revised]
PHST- 2021/09/13 00:00 [accepted]
PHST- 2021/09/27 06:00 [pubmed]
PHST- 2022/01/27 06:00 [medline]
PHST- 2021/09/26 20:54 [entrez]
AID - S0753-3322(21)00987-2 [pii]
AID - 10.1016/j.biopha.2021.112203 [doi]
PST - ppublish
SO  - Biomed Pharmacother. 2021 Nov;143:112203. doi: 10.1016/j.biopha.2021.112203. Epub 
      2021 Sep 24.