PMID- 34565155
OWN - NLM
STAT- MEDLINE
DCOM- 20210928
LR  - 20210928
IS  - 0001-723X (Print)
IS  - 0001-723X (Linking)
VI  - 65
IP  - 3
DP  - 2021
TI  - Development and evaluation of multi-epitope protein p72 (MeP72) for the 
      serodiagnosis of African swine fever.
PG  - 273-278
LID - 10.4149/av_2021_304 [doi]
AB  - African swine fever (ASF) is an acute and severe infectious disease that 
      seriously endangers the global porcine industry. In order to develop ASF 
      serodiagnostic reagents with high specificity and sensitivity, in the present 
      study, the antigenic epitopes of P72 protein of African swine fever virus (ASFV) 
      were analyzed, and the ASFV multi-epitope fusion gene MeP72 in tandem with the 
      dominant linear epitopes was constructed. The recombinant multi-epitope fusion 
      MeP72 (reMeP72) was prepared in Escherichia coli. A colloidal gold-based 
      immunochromatographic assay (CGIA) based on reMeP72 was developed for the 
      detection of antibodies against ASFV. A total of 139 pig clinical serum samples 
      were used for assessment of the potential diagnostic value of reMeP72. The 
      results showed that CGIA did not cross-react with positive sera of viruses, such 
      as classical swine fever virus (CSFV), porcine circovirus type 2 (PCV2), 
      pseudorabies virus (PRV), porcine reproductive and respiratory syndrome virus 
      (PRRSV) and swine influenza virus (SIV), showing high specificity. Sensitivity 
      analysis showed that CGIA could detect ASFV-positive serum at a dilution of 1:64. 
      Compared with commercial ASFV kits, the sensitivity and specificity of ASFV CGIA 
      based on reMeP72 protein were 85.7% and 97.6%, respectively. The agreement rate 
      of the two methods was 96.4%, showing a good detection performance. The results 
      indicated that the reMeP72 was of potential value for the serodiagnosis of ASF. 
      Keywords: African swine fever virus; P72 gene; antigenic protein; colloidal 
      gold-based immunochromatographic assay.
FAU - Zhang, Xingxing
AU  - Zhang X
FAU - Guo, Jing
AU  - Guo J
FAU - Wang, Lixia
AU  - Wang L
FAU - Li, Zhiyuan
AU  - Li Z
FAU - Liu, Yucheng
AU  - Liu Y
FAU - Tian, Lulu
AU  - Tian L
FAU - Xiao, Chencheng
AU  - Xiao C
FAU - Li, Yanfang
AU  - Li Y
FAU - Cai, Xuepeng
AU  - Cai X
FAU - Meng, Qingling
AU  - Meng Q
FAU - Qiao, Jun
AU  - Qiao J
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Acta Virol
JT  - Acta virologica
JID - 0370401
RN  - 0 (Epitopes)
SB  - IM
MH  - *African Swine Fever/diagnosis
MH  - *African Swine Fever Virus/genetics
MH  - Animals
MH  - Epitopes
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Serologic Tests
MH  - Swine
EDAT- 2021/09/28 06:00
MHDA- 2021/09/29 06:00
CRDT- 2021/09/27 05:23
PHST- 2021/09/27 05:23 [entrez]
PHST- 2021/09/28 06:00 [pubmed]
PHST- 2021/09/29 06:00 [medline]
AID - 10.4149/av_2021_304 [doi]
PST - ppublish
SO  - Acta Virol. 2021;65(3):273-278. doi: 10.4149/av_2021_304.