PMID- 34566328
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220427
IS  - 0972-8988 (Print)
IS  - 2231-0916 (Electronic)
IS  - 0972-8988 (Linking)
VI  - 14
IP  - 8
DP  - 2021 Aug
TI  - Wound healing potential of human umbilical cord mesenchymal stem cell conditioned 
      medium: An in vitro and in vivo study in diabetes-induced rats.
PG  - 2109-2117
LID - 10.14202/vetworld.2021.2109-2117 [doi]
AB  - BACKGROUND AND AIM: Human umbilical cord mesenchymal stem cells (hUC-MSCs) and 
      its conditioned medium (CM) promote wound healing. This study investigated the 
      wound healing potential of hUC-MSC CM in vitro and in vivo using diabetic animal 
      models. MATERIALS AND METHODS: The CM from hUC-MSC CM prepared under hypoxic 
      conditions (hypoxic hUC-MSC) was evaluated for stimulating rat fibroblast growth, 
      collagen production (in vitro), and wound healing in animal models (in vivo). An 
      excision wound on the dorsal side of the diabetes-induced rats was established, 
      and the rats were randomly divided into non-treatment, antibiotic, and hypoxic 
      hUC-MSC CM groups. The cell number of fibroblasts and collagen secretion was 
      evaluated and compared among the groups in an in vitro study. By contrast, wound 
      size reduction, width of re-epithelialization, and the collagen formation area 
      were assessed and compared among the groups in an in vivo study. RESULTS: CM 
      under hypoxic conditions contained a higher concentration of wound 
      healing-related growth factors. Hypoxic hUC-MSC CM could facilitate fibroblast 
      cell growth and collagen synthesis, although not significant compared with the 
      control group. Re-epithelialization and collagen production were higher in the 
      hUC-MSC CM group than in the antibiotic and non-treatment groups. CONCLUSION: 
      Hypoxic hUC-MSC CM possessed more positive effects on the wound healing process 
      based on re-epithelialization and collagen formation than antibiotic treatment 
      did.
CI  - Copyright: (c) Hendrawan, et al.
FAU - Hendrawan, Siufui
AU  - Hendrawan S
AD  - Department of Biochemistry and Molecular Biology, Faculty of Medicine, 
      Tarumanagara University, 11440, Jakarta, Indonesia.
AD  - Tarumanagara Human Cell Technology Laboratory, Tarumanagara University, 11440, 
      Jakarta, Indonesia.
FAU - Kusnadi, Yuyus
AU  - Kusnadi Y
AD  - Stem Cell Division, Stem Cell and Cancer Institute, PT. Kalbe Farma, Tbk., 10510, 
      Jakarta, Indonesia.
FAU - Lagonda, Christine Ayu
AU  - Lagonda CA
AD  - Stem Cell Division, Stem Cell and Cancer Institute, PT. Kalbe Farma, Tbk., 10510, 
      Jakarta, Indonesia.
FAU - Fauza, Dilafitria
AU  - Fauza D
AD  - Stem Cell Division, Stem Cell and Cancer Institute, PT. Kalbe Farma, Tbk., 10510, 
      Jakarta, Indonesia.
FAU - Lheman, Jennifer
AU  - Lheman J
AD  - Tarumanagara Human Cell Technology Laboratory, Tarumanagara University, 11440, 
      Jakarta, Indonesia.
FAU - Budi, Erwin
AU  - Budi E
AD  - Tarumanagara Human Cell Technology Laboratory, Tarumanagara University, 11440, 
      Jakarta, Indonesia.
FAU - Manurung, Brian Saputra
AU  - Manurung BS
AD  - Tarumanagara Human Cell Technology Laboratory, Tarumanagara University, 11440, 
      Jakarta, Indonesia.
FAU - Baer, Hans Ulrich
AU  - Baer HU
AD  - Baermed, Centre of Abdominal Surgery, Hirslanden Clinic, 2501, Zurich, 
      Switzerland.
AD  - Department of Visceral and Transplantation Surgery, University of Bern, 3012, 
      Bern, Switzerland.
FAU - Tansil Tan, Sukmawati
AU  - Tansil Tan S
AD  - Department of Dermatovenereology, Faculty of Medicine, Tarumanagara University, 
      11440, Jakarta, Indonesia.
LA  - eng
PT  - Journal Article
DEP - 20210817
PL  - India
TA  - Vet World
JT  - Veterinary world
JID - 101504872
PMC - PMC8448625
OTO - NOTNLM
OT  - conditioned medium
OT  - diabetic induced rat
OT  - human umbilical cord mesenchymal stem cells
OT  - wound healing
EDAT- 2021/09/28 06:00
MHDA- 2021/09/28 06:01
PMCR- 2021/08/01
CRDT- 2021/09/27 05:57
PHST- 2021/03/01 00:00 [received]
PHST- 2021/07/08 00:00 [accepted]
PHST- 2021/09/27 05:57 [entrez]
PHST- 2021/09/28 06:00 [pubmed]
PHST- 2021/09/28 06:01 [medline]
PHST- 2021/08/01 00:00 [pmc-release]
AID - Vetworld-14-2109 [pii]
AID - 10.14202/vetworld.2021.2109-2117 [doi]
PST - ppublish
SO  - Vet World. 2021 Aug;14(8):2109-2117. doi: 10.14202/vetworld.2021.2109-2117. Epub 
      2021 Aug 17.