PMID- 34566965
OWN - NLM
STAT- MEDLINE
DCOM- 20211220
LR  - 20211220
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 12
DP  - 2021
TI  - Unraveling the Heterogeneity and Ontogeny of Dendritic Cells Using Single-Cell 
      RNA Sequencing.
PG  - 711329
LID - 10.3389/fimmu.2021.711329 [doi]
LID - 711329
AB  - Dendritic cells (DCs) play essential roles in innate and adaptive immunity and 
      show high heterogeneity and intricate ontogeny. Advances in high-throughput 
      sequencing technologies, particularly single-cell RNA sequencing (scRNA-seq), 
      have improved the understanding of DC subsets. In this review, we discuss in 
      detail the remarkable perspectives in DC reclassification and ontogeny as 
      revealed by scRNA-seq. Moreover, the heterogeneity and multifunction of DCs 
      during diseases as determined by scRNA-seq are described. Finally, we provide 
      insights into the challenges and future trends in scRNA-seq technologies and DC 
      research.
CI  - Copyright (c) 2021 Chen, Zhu, Yang and Su.
FAU - Chen, Binyao
AU  - Chen B
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Zhu, Lei
AU  - Zhu L
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Yang, Shizhao
AU  - Yang S
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Su, Wenru
AU  - Su W
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20210909
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
SB  - IM
MH  - Cell Lineage
MH  - Dendritic Cells/*classification/cytology/immunology
MH  - Humans
MH  - Lymphocytes, Tumor-Infiltrating/immunology
MH  - Neoplasms/immunology/therapy
MH  - Sequence Analysis, RNA/*methods
MH  - Single-Cell Analysis/*methods
MH  - Tumor Microenvironment
PMC - PMC8458576
OTO - NOTNLM
OT  - cellular heterogeneity
OT  - dendritic cells
OT  - single-cell RNA sequencing
OT  - the ontogeny of dendritic cells
OT  - tumor-infiltrating dendritic cells
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/09/28 06:00
MHDA- 2021/12/21 06:00
PMCR- 2021/01/01
CRDT- 2021/09/27 06:04
PHST- 2021/05/18 00:00 [received]
PHST- 2021/08/23 00:00 [accepted]
PHST- 2021/09/27 06:04 [entrez]
PHST- 2021/09/28 06:00 [pubmed]
PHST- 2021/12/21 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2021.711329 [doi]
PST - epublish
SO  - Front Immunol. 2021 Sep 9;12:711329. doi: 10.3389/fimmu.2021.711329. eCollection 
      2021.