PMID- 34567821
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230921
IS  - 2090-8865 (Print)
IS  - 2090-8873 (Electronic)
IS  - 2090-8873 (Linking)
VI  - 2021
DP  - 2021
TI  - Establishment of the Quantitative Analysis of Multiindex in Euphorbia lathyris by 
      the Single Marker Method for Euphorbia lathyris Based on the Quality by Design 
      Concept.
PG  - 4311934
LID - 10.1155/2021/4311934 [doi]
LID - 4311934
AB  - METHODS: The influences of methanol proportion, flow rate, column temperature, 
      and injection volume in the mobile phase on the chromatographic resolution of 
      chromatographic peak of euphorbia factor L1 were experimentally studied via 
      Plackett-Burman design, and the key analysis parameters were screened out; the 
      key analysis parameters were optimized through the central composite design, and 
      the chromatographic analysis conditions were established. Euphorbia factor L1 was 
      taken as the internal reference to construct the relative correction factors for 
      L3 and L4 relative to L1, and their contents were calculated, thus realizing the 
      QAMS. Meanwhile, the euphorbia factor L3 and euphorbia factor L4 were determined 
      using the external standard method, and the differences of values measured by the 
      external standard method from the values predicted by the QAMS method were 
      compared, in an effort to verify the accuracy and feasibility of the QAMS method. 
      RESULTS: The methanol proportion and column temperature in the mobile phase were 
      the key analysis parameters (P < 0.05), and the chromatographic conditions were 
      determined as follows. The methanol/water ratio, column temperature, detection 
      wavelength, flow rate, and injection volume were 60 : 40, 30 degrees C, 275 nm, 
      1.0 mL/min, and 10 muL, respectively. A total of 20 batches of samples were 
      determined by the QAMS method and external standard method; the relative standard 
      deviations (RSDs) of L3 and L4 determination results were less than 2.0%, without 
      any significant difference. CONCLUSION: The QbD-based QAMS method can be used to 
      determine the contents of euphorbia factor L3 and euphorbia factor L4 in 
      Euphorbia lathyris L., and it is accurate and feasible.
CI  - Copyright (c) 2021 Feng Xuehua et al.
FAU - Xuehua, Feng
AU  - Xuehua F
AUID- ORCID: 0000-0002-7185-8261
AD  - College of Pharmacy, Anhui Xinhua University, Hefei 230088, China.
FAU - Guangjiao, Zhou
AU  - Guangjiao Z
AUID- ORCID: 0000-0001-5538-8338
AD  - Bozhou Chinese Medicine Institute, Anhui Academy of Chinese Medicine, Bozhou 
      236800, China.
FAU - Ali, Tao
AU  - Ali T
AUID- ORCID: 0000-0003-2911-2471
AD  - College of Pharmacy, Anhui Xinhua University, Hefei 230088, China.
LA  - eng
PT  - Journal Article
DEP - 20210917
PL  - Egypt
TA  - J Anal Methods Chem
JT  - Journal of analytical methods in chemistry
JID - 101575377
PMC - PMC8463240
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2021/09/28 06:00
MHDA- 2021/09/28 06:01
PMCR- 2021/09/17
CRDT- 2021/09/27 06:12
PHST- 2021/07/27 00:00 [received]
PHST- 2021/08/27 00:00 [revised]
PHST- 2021/09/01 00:00 [accepted]
PHST- 2021/09/27 06:12 [entrez]
PHST- 2021/09/28 06:00 [pubmed]
PHST- 2021/09/28 06:01 [medline]
PHST- 2021/09/17 00:00 [pmc-release]
AID - 10.1155/2021/4311934 [doi]
PST - epublish
SO  - J Anal Methods Chem. 2021 Sep 17;2021:4311934. doi: 10.1155/2021/4311934. 
      eCollection 2021.