PMID- 34569011
OWN - NLM
STAT- MEDLINE
DCOM- 20220617
LR  - 20220617
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 200
IP  - 7
DP  - 2022 Jul
TI  - The Effects of Sodium Fluoride (NaF) Treatment on the PI3K/Akt Signal Pathway in 
      NRK-52E Cells.
PG  - 3294-3302
LID - 10.1007/s12011-021-02927-4 [doi]
AB  - The effects of the element fluorine on the phosphoinositide-3-kinase-protein 
      kinase B/Akt (PI3K/Akt) pathway has a significant role in regulation of 
      intracellular molecular mechanisms. NRK-52E rat kidney epithelial cell line was 
      selected as the material of the study. NaF was used as the fluorine source in the 
      study. The NaF dose was determined with the MTT assay. The NaF concentrations 
      were determined as the proliferation concentration of 10 muM and IC(25) (2250 muM) 
      and IC(50) (4250 muM) for 24 h. In the study, the erb-b2 receptor tyrosine kinase 
      2 (ERBB2), phosphoinositide-3-kinase (PI3K), Protein kinase B (PKB,Akt), 
      Mammalian target of rapamycin (mTOR), and the Tumor protein 53 (TP53) genes were 
      considered as the target genes. NaF concentration was administered on the cells. 
      Total mRNA was isolated. mRNAs were turned into cDNA. The expression levels of 
      the target genes were determined by RT-qPCR method. According to the results 
      obtained in the study, the low NaF concentration increased the expression levels 
      of the ERBB2, PI3K, and Akt genes, while the higher concentrations did not 
      significantly affect these levels. The expression of mTOR decreased at all given 
      concentrations. The expression of the TP53 gene did not change at the low 
      concentration, while it increased at the high concentrations. Based on the 
      results, it may be stated that fluorine may inhibit the kinase enzymes in the 
      PI3K/Akt pathway. In summary, in the pathogenesis of the cell damage caused by 
      fluorine in the NRK-52E cell line, the PI3K/Akt/mTOR pathway is an important 
      signal pathway.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Korkmaz, Riskiye
AU  - Korkmaz R
AD  - Department of Biochemstry, Faculty of Veterinary Medicine, Van Yuzuncu Yil 
      University, Van, Turkey.
FAU - Yuksek, Veysel
AU  - Yuksek V
AUID- ORCID: 0000-0001-7432-4989
AD  - Department of Medical Laboratory, Ozalp Vocational High School, Van Yuzuncu Yil 
      University, Van, Turkey. veyselyuksek@yyu.edu.tr.
FAU - Dede, Semiha
AU  - Dede S
AD  - Department of Biochemstry, Faculty of Veterinary Medicine, Van Yuzuncu Yil 
      University, Van, Turkey.
LA  - eng
GR  - TYL-2019-8226/scientific research projects presidency of van yuzuncu yil 
      university/
PT  - Journal Article
DEP - 20210927
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Phosphatidylinositols)
RN  - 0 (RNA, Messenger)
RN  - 284SYP0193 (Fluorine)
RN  - 8ZYQ1474W7 (Sodium Fluoride)
RN  - EC 2.7.1.137 (Phosphatidylinositol 3-Kinase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Fluorine/pharmacology
MH  - Mammals/metabolism
MH  - Phosphatidylinositol 3-Kinase/metabolism
MH  - *Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphatidylinositols/pharmacology
MH  - *Proto-Oncogene Proteins c-akt/metabolism
MH  - RNA, Messenger
MH  - Rats
MH  - Signal Transduction
MH  - Sodium Fluoride/pharmacology
MH  - TOR Serine-Threonine Kinases/metabolism
OTO - NOTNLM
OT  - Akt
OT  - NRK-52E cell line
OT  - NaF
OT  - P53
OT  - PI3K
EDAT- 2021/09/28 06:00
MHDA- 2022/06/18 06:00
CRDT- 2021/09/27 06:30
PHST- 2021/07/31 00:00 [received]
PHST- 2021/09/09 00:00 [accepted]
PHST- 2021/09/28 06:00 [pubmed]
PHST- 2022/06/18 06:00 [medline]
PHST- 2021/09/27 06:30 [entrez]
AID - 10.1007/s12011-021-02927-4 [pii]
AID - 10.1007/s12011-021-02927-4 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2022 Jul;200(7):3294-3302. doi: 10.1007/s12011-021-02927-4. 
      Epub 2021 Sep 27.