PMID- 34570407
OWN - NLM
STAT- MEDLINE
DCOM- 20220427
LR  - 20220722
IS  - 1365-2958 (Electronic)
IS  - 0950-382X (Print)
IS  - 0950-382X (Linking)
VI  - 117
IP  - 3
DP  - 2022 Mar
TI  - Precision-cut lung slices: A powerful ex vivo model to investigate respiratory 
      infectious diseases.
PG  - 578-588
LID - 10.1111/mmi.14817 [doi]
AB  - Respiratory infections are a leading cause of mortality worldwide. Most of the 
      research on the underlying disease mechanisms is based on cell culture, organoid, 
      or surrogate animal models. Although these provide important insights, they have 
      limitations. Cell culture models fail to recapitulate cellular interactions in 
      the lung and animal models often do not permit high-throughput analysis of drugs 
      or pathogen isolates; hence, there is a need for improved, scalable models. 
      Precision-cut lung slices (PCLS), small, uniform tissue slices generated from 
      animal or human lungs are increasingly recognized and employed as an ex vivo 
      organotypic model. PCLS retain remarkable cellular complexity and the 
      architecture of the lung, providing a platform to investigate respiratory 
      pathogens in a near-native environment. Here, we review the generation and 
      features of PCLS, their use to investigate the pathogenesis of viral and 
      bacterial pathogens, and highlight their potential to advance respiratory 
      infection research in the future.
CI  - (c) 2021 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.
FAU - Viana, Flavia
AU  - Viana F
AUID- ORCID: 0000-0001-6286-4902
AD  - Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, 
      Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK.
FAU - O'Kane, Cecilia M
AU  - O'Kane CM
AUID- ORCID: 0000-0002-7138-5396
AD  - Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, 
      Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK.
FAU - Schroeder, Gunnar N
AU  - Schroeder GN
AUID- ORCID: 0000-0002-0412-7741
AD  - Wellcome-Wolfson Institute for Experimental Medicine, School of Medicine, 
      Dentistry and Biomedical Sciences, Queen's University Belfast, Belfast, UK.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20211031
PL  - England
TA  - Mol Microbiol
JT  - Molecular microbiology
JID - 8712028
SB  - IM
MH  - Animals
MH  - *Communicable Diseases/pathology
MH  - *Lung/microbiology/pathology
PMC - PMC9298270
OTO - NOTNLM
OT  - ex vivo
OT  - infection
OT  - organotypic models
OT  - precision-cut lung slices
OT  - respiratory infections
COIS- FV and GNS declare that they have no conflicts of interest with the contents of 
      this article. COK has received consultancy fees from Insmed for the treatment of 
      pulmonary non tuberculous mycobacterial infection.
EDAT- 2021/09/28 06:00
MHDA- 2022/04/28 06:00
PMCR- 2022/07/20
CRDT- 2021/09/27 12:40
PHST- 2021/09/21 00:00 [received]
PHST- 2021/09/23 00:00 [accepted]
PHST- 2021/09/28 06:00 [pubmed]
PHST- 2022/04/28 06:00 [medline]
PHST- 2021/09/27 12:40 [entrez]
PHST- 2022/07/20 00:00 [pmc-release]
AID - MMI14817 [pii]
AID - 10.1111/mmi.14817 [doi]
PST - ppublish
SO  - Mol Microbiol. 2022 Mar;117(3):578-588. doi: 10.1111/mmi.14817. Epub 2021 Oct 31.