PMID- 34570665
OWN - NLM
STAT- MEDLINE
DCOM- 20230301
LR  - 20230301
IS  - 2000-6764 (Electronic)
IS  - 2000-6764 (Linking)
VI  - 57
IP  - 1-6
DP  - 2023 Feb-Dec
TI  - Human umbilical cord mesenchymal stem cells prevent glucocorticoid-induced 
      osteonecrosis of the femoral head by promoting angiogenesis.
PG  - 71-77
LID - 10.1080/2000656X.2021.1981352 [doi]
AB  - The impairment of angiogenesis is an outstanding pathogenic characteristic of 
      glucocorticoid (GC)-induced osteonecrosis of the femoral head (ONFH). Human 
      umbilical cord mesenchymal stem cells (hUC-MSCs) have been used in several 
      diseases models, which were reported to be involved in the angiogenesis. However, 
      whether hUC-MSCs suppress the GC-induced ONFH via promoting angiogenesis is still 
      unclear. hUC-MSCs were isolated from the Wharton's jelly using the explant 
      culture method. A GC-induced ONFH model was established in vitro and in vivo. The 
      angiogenesis, proliferation and migration ability of HMECs were determined using 
      the tube-forming, CCK-8, transwell and scratching assays in vitro. The protective 
      role of hUC-MSCs in GC-induced ONFH was evaluated using micro-CT scanning and 
      histological, immunohistochemical (IHC) and Quantitative reverse 
      transcription-polymerase chain reaction (qRT-PCR) assays in vivo. The results 
      showed that hUC-MSCs treatment improved the tube-forming, proliferation and 
      migration ability of HMECs in vitro. Moreover, hUC-MSCs treatment enhanced the 
      integrity of trabecular bone of the femoral head, and the tube-forming ability 
      in vivo. hUC-MSCs prevent the femoral head against necrosis and damage caused by 
      GCs though promoting angiogenesis.
FAU - Tian, Gang
AU  - Tian G
AD  - Department of Orthopedics, Weihai Central Hospital, Affiliated to Qingdao 
      University & Qingdao University, Weihai, China.
FAU - Liu, Chuanjie
AU  - Liu C
AD  - Weihai Key Laboratory of Autoimmunity & Central Laboratory, Weihai Central 
      Hospital, Affiliated to Qingdao University & Qingdao University, Weihai, China.
FAU - Wang, Haitao
AU  - Wang H
AD  - Department of Trauma Surgery, Weihai Central Hospital Affiliated to Qingdao 
      University, Weihai, China.
FAU - Yu, Zhiping
AU  - Yu Z
AD  - Department of Sports Medicine, Weihai Central Hospital Affiliated to Qingdao 
      University, Weihai, China.
FAU - Huang, Jian
AU  - Huang J
AD  - Department of Sports Medicine, Limin Hospital, Weihai City Central Hospital, 
      Weihai, China.
FAU - Gong, Qi
AU  - Gong Q
AD  - Weihai Key Laboratory of Autoimmunity & Central Laboratory, Weihai Central 
      Hospital, Affiliated to Qingdao University & Qingdao University, Weihai, China.
FAU - Zhang, Daoqiang
AU  - Zhang D
AD  - Weihai Key Laboratory of Autoimmunity & Central Laboratory, Weihai Central 
      Hospital, Affiliated to Qingdao University & Qingdao University, Weihai, China.
FAU - Cong, Haibo
AU  - Cong H
AD  - Department of Orthopedics, Weihai Central Hospital, Affiliated to Qingdao 
      University & Weihai Key Laboratory of Autoimmunity, Qingdao University, Weihai, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20210927
PL  - Sweden
TA  - J Plast Surg Hand Surg
JT  - Journal of plastic surgery and hand surgery
JID - 101534130
RN  - 0 (Glucocorticoids)
SB  - IM
MH  - Humans
MH  - Glucocorticoids
MH  - Femur Head
MH  - *Mesenchymal Stem Cells
MH  - *Osteonecrosis
MH  - Umbilical Cord
OTO - NOTNLM
OT  - Microvascular
OT  - Necrosis
OT  - glucocorticoid
OT  - hUC-MSCs
OT  - osteonecrosis of the femoral head
EDAT- 2021/09/28 06:00
MHDA- 2023/03/03 06:00
CRDT- 2021/09/27 17:17
PHST- 2021/09/28 06:00 [pubmed]
PHST- 2023/03/03 06:00 [medline]
PHST- 2021/09/27 17:17 [entrez]
AID - 10.1080/2000656X.2021.1981352 [doi]
PST - ppublish
SO  - J Plast Surg Hand Surg. 2023 Feb-Dec;57(1-6):71-77. doi: 
      10.1080/2000656X.2021.1981352. Epub 2021 Sep 27.