PMID- 34571879
OWN - NLM
STAT- MEDLINE
DCOM- 20211117
LR  - 20211117
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 9
DP  - 2021 Aug 28
TI  - Degenerated, Undifferentiated, Rearranged, Lost: High Variability of Sex 
      Chromosomes in Geometridae (Lepidoptera) Identified by Sex Chromatin.
LID - 10.3390/cells10092230 [doi]
LID - 2230
AB  - Sex chromatin is a conspicuous body that occurs in polyploid nuclei of most 
      lepidopteran females and consists of numerous copies of the W sex chromosome. It 
      is also a cytogenetic tool used to rapidly assess the W chromosome presence in 
      Lepidoptera. However, certain chromosomal features could disrupt the formation of 
      sex chromatin and lead to the false conclusion that the W chromosome is absent in 
      the respective species. Here we tested the sex chromatin presence in 50 species 
      of Geometridae. In eight selected species with either missing, atypical, or 
      normal sex chromatin patterns, we performed a detailed karyotype analysis by 
      means of comparative genomic hybridization (CGH) and fluorescence in situ 
      hybridization (FISH). The results showed a high diversity of W chromosomes and 
      clarified the reasons for atypical sex chromatin, including the absence or poor 
      differentiation of W, rearrangements leading to the neo-W emergence, possible 
      association with the nucleolus, and the existence of multiple W chromosomes. In 
      two species, we detected intraspecific variability in the sex chromatin status 
      and sex chromosome constitution. We show that the sex chromatin is not a 
      sufficient marker of the W chromosome presence, but it may be an excellent tool 
      to pinpoint species with atypical sex chromosomes.
FAU - Hejnickova, Martina
AU  - Hejnickova M
AUID- ORCID: 0000-0002-9303-305X
AD  - Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske 
      Budejovice, Czech Republic.
AD  - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, 
      Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
FAU - Dalikova, Martina
AU  - Dalikova M
AUID- ORCID: 0000-0002-0266-8894
AD  - Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske 
      Budejovice, Czech Republic.
AD  - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, 
      Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
FAU - Potocky, Pavel
AU  - Potocky P
AD  - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, 
      Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
FAU - Tammaru, Toomas
AU  - Tammaru T
AD  - Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, 51014 
      Tartu, Estonia.
FAU - Trehubenko, Marharyta
AU  - Trehubenko M
AD  - Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske 
      Budejovice, Czech Republic.
AD  - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, 
      Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
FAU - Kubickova, Svatava
AU  - Kubickova S
AUID- ORCID: 0000-0002-1006-9453
AD  - Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
FAU - Marec, Frantisek
AU  - Marec F
AUID- ORCID: 0000-0002-6745-5603
AD  - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, 
      Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
FAU - Zrzava, Magda
AU  - Zrzava M
AUID- ORCID: 0000-0001-8482-9614
AD  - Faculty of Science, University of South Bohemia, Branisovska 1760, 370 05 Ceske 
      Budejovice, Czech Republic.
AD  - Biology Centre of the Czech Academy of Sciences, Institute of Entomology, 
      Branisovska 31, 370 05 Ceske Budejovice, Czech Republic.
LA  - eng
GR  - 17-13713S/Grantova Agentura Ceske Republiky/
GR  - 20-13784S/Grantova Agentura Ceske Republiky/
GR  - 013/2020/P/Grantova agentura JU/
GR  - 20-20650Y/Grantova Agentura Ceske Republiky/
GR  - RO 0520/Ministerstvo Skolstvi, Mladeze a Telovychovy/
GR  - PRG741/Estonian Research Competency Council/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210828
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
SB  - IM
MH  - Animals
MH  - Comparative Genomic Hybridization
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Male
MH  - Moths/genetics
MH  - Sex Chromatin/*metabolism
MH  - Sex Chromosomes/*genetics
MH  - Species Specificity
PMC - PMC8468057
OTO - NOTNLM
OT  - Geometridae
OT  - Lepidoptera
OT  - W chromosome
OT  - comparative genomic hybridization
OT  - intraspecific chromosomal variability
OT  - neo-sex chromosomes
OT  - sex chromatin
OT  - sex chromosome evolution
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/29 06:00
MHDA- 2021/11/18 06:00
PMCR- 2021/08/28
CRDT- 2021/09/28 01:04
PHST- 2021/07/28 00:00 [received]
PHST- 2021/08/19 00:00 [revised]
PHST- 2021/08/23 00:00 [accepted]
PHST- 2021/09/28 01:04 [entrez]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2021/11/18 06:00 [medline]
PHST- 2021/08/28 00:00 [pmc-release]
AID - cells10092230 [pii]
AID - cells-10-02230 [pii]
AID - 10.3390/cells10092230 [doi]
PST - epublish
SO  - Cells. 2021 Aug 28;10(9):2230. doi: 10.3390/cells10092230.