PMID- 34571975
OWN - NLM
STAT- MEDLINE
DCOM- 20211119
LR  - 20211119
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 10
IP  - 9
DP  - 2021 Sep 6
TI  - Sinapic Acid Controls Inflammation by Suppressing NLRP3 Inflammasome Activation.
LID - 10.3390/cells10092327 [doi]
LID - 2327
AB  - A natural phenolic acid compound, sinapic acid (SA), is a cinnamic acid 
      derivative that contains 3,5-dimethoxyl and 4-hydroxyl substitutions in the 
      phenyl ring of cinnamic acid. SA is present in various orally edible natural 
      herbs and cereals and is reported to have antioxidant, antitumor, 
      anti-inflammatory, antibacterial, and neuroprotective activities. Although the 
      anti-inflammatory function of SA has been reported, the effect of SA on the 
      NOD-like receptor pyrin domain-containing 3 (NLRP3) inflammasome has not been 
      explored. In the present study, to elucidate the anti-inflammatory mechanism of 
      SA, we examined whether SA modulates the NLRP3 inflammasome. We found that SA 
      blocked caspase-1 activation and IL-1beta secretion by inhibiting NLRP3 inflammasome 
      activation in bone marrow-derived macrophages (BMDMs). Apoptosis-associated 
      speck-like protein containing CARD (ASC) pyroptosome formation was consistently 
      blocked by SA treatment. SA specifically inhibited NLRP3 activation but not the 
      NLRC4 or AIM2 inflammasomes. In addition, SA had no significant effect on the 
      priming phase of the NLRP3 inflammasome, such as pro-IL-1beta and NLRP3 inflammasome 
      expression levels. Moreover, we found that SA attenuated IL-1beta secretion in 
      LPS-induced systemic inflammation in mice and reduced lethality from endotoxic 
      shock. Our findings suggest that the natural compound SA has potential 
      therapeutic value for the suppression of NLRP3 inflammasome-associated 
      inflammatory diseases.
FAU - Lee, Eun Hye
AU  - Lee EH
AD  - Department of Molecular Bioscience, College of Biomedical Science, Institute of 
      Bioscience & Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
FAU - Shin, Jin Hak
AU  - Shin JH
AD  - Department of Molecular Bioscience, College of Biomedical Science, Institute of 
      Bioscience & Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
FAU - Kim, Seon Sook
AU  - Kim SS
AD  - Department of Molecular Bioscience, College of Biomedical Science, Institute of 
      Bioscience & Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
FAU - Seo, Su Ryeon
AU  - Seo SR
AUID- ORCID: 0000-0002-4830-4965
AD  - Department of Molecular Bioscience, College of Biomedical Science, Institute of 
      Bioscience & Biotechnology, Kangwon National University, Chuncheon 24341, Korea.
LA  - eng
GR  - 2018R1A2B6006286/National Research Foundation of Korea/
GR  - 2021R1A2C1008170/National Research Foundation of Korea/
GR  - 2019/Kangwon National University/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210906
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Coumaric Acids)
RN  - 0 (Inflammasomes)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 68A28V6010 (sinapinic acid)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Apoptosis/drug effects
MH  - Caspase 1/metabolism
MH  - Cells, Cultured
MH  - Coumaric Acids/*pharmacology
MH  - Inflammasomes/*drug effects/metabolism
MH  - Inflammation/*drug therapy/*metabolism
MH  - Interleukin-1beta/metabolism
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages/drug effects/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/*metabolism
MH  - RAW 264.7 Cells
PMC - PMC8470482
OTO - NOTNLM
OT  - NLRP3 inflammasome
OT  - bone marrow-derived macrophages
OT  - inflammation
OT  - natural compound
OT  - sinapic acid
COIS- The authors declare that there are no conflict of interest.
EDAT- 2021/09/29 06:00
MHDA- 2021/11/20 06:00
PMCR- 2021/09/06
CRDT- 2021/09/28 01:04
PHST- 2021/08/06 00:00 [received]
PHST- 2021/08/31 00:00 [revised]
PHST- 2021/09/03 00:00 [accepted]
PHST- 2021/09/28 01:04 [entrez]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2021/11/20 06:00 [medline]
PHST- 2021/09/06 00:00 [pmc-release]
AID - cells10092327 [pii]
AID - cells-10-02327 [pii]
AID - 10.3390/cells10092327 [doi]
PST - epublish
SO  - Cells. 2021 Sep 6;10(9):2327. doi: 10.3390/cells10092327.