PMID- 34573362
OWN - NLM
STAT- MEDLINE
DCOM- 20220214
LR  - 20220214
IS  - 2073-4425 (Electronic)
IS  - 2073-4425 (Linking)
VI  - 12
IP  - 9
DP  - 2021 Aug 31
TI  - Single Nucleotide Polymorphisms of Immunity-Related Genes and Their Effects on 
      Immunophenotypes in Different Pig Breeds.
LID - 10.3390/genes12091377 [doi]
LID - 1377
AB  - Enhancing resistance and tolerance to pathogens remains an important selection 
      objective in the production of livestock animals. Single nucleotide polymorphisms 
      (SNPs) vary gene expression at the transcriptional level, influencing an 
      individual's immune regulation and susceptibility to diseases. In this study, we 
      investigated the distribution of SNP sites in immune-related genes and their 
      correlations with cell surface markers of immune cells within purebred (Taiwan 
      black, Duroc, Landrace and Yorkshire) and crossbred (Landrace-Yorkshire) pigs. 
      Thirty-nine SNPs of immune-related genes, including 11 cytokines, 5 chemokines 
      and 23 Toll-like receptors (TLRs) (interferon-alpha and gamma (IFN-alpha, gamma), tumor necrosis 
      factor-alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), 
      Monocyte chemoattractant protein-1 (MCP-1) and TLR3, TLR4, TLR7, TLR8, and TLR9) 
      were selected, and the percentages of positive cells with five cell surface 
      markers of CD4, CD8, CD80/86, MHCI, and MHCII were analyzed. There were 28 SNPs 
      that were significantly different among breeds, particularly between Landrace and 
      Taiwan black. For instance, the frequency of SNP1 IFN-alpha -235A/G in Taiwan black 
      and Landrace was 11.11% and 96.15%, respectively. In addition, 18 SNPs 
      significantly correlated with the expression of cell surface markers, including 
      CD4, CD8, CD80/86, and MHCII. The percentage of CD4+ (39.27%) in SNP33 TLR-8 
      543C/C was significantly higher than those in A/C (24.34%), at p < 0.05. 
      Together, our findings show that Taiwan black pigs had a unique genotype 
      distribution, whereas Landrace and Yorkshire had a more similar genotype 
      distribution. Thus, an understanding of the genetic uniqueness of each breed 
      could help to identify functionally important SNPs in immunoregulation.
FAU - Chen, Ann Ying-An
AU  - Chen AY
AUID- ORCID: 0000-0003-4046-0346
AD  - Research Center for Animal Biologics, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
FAU - Huang, Chao-Wei
AU  - Huang CW
AD  - Department of Tropical Agriculture and International Cooperation, National 
      Pingtung University of Science and Technology, Neipu, Pingtung 912, Taiwan.
FAU - Liu, Shyh-Hwa
AU  - Liu SH
AD  - Department of Animal Science, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
FAU - Liu, An-Chi
AU  - Liu AC
AD  - Research Center for Animal Biologics, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
FAU - Chaung, Hso-Chi
AU  - Chaung HC
AD  - Research Center for Animal Biologics, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Neipu, Pingtung 912, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210831
PL  - Switzerland
TA  - Genes (Basel)
JT  - Genes
JID - 101551097
RN  - 0 (Biomarkers)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Disease Resistance/*genetics
MH  - *Genetic Predisposition to Disease
MH  - Immunophenotyping
MH  - Polymorphism, Single Nucleotide
MH  - Selective Breeding
MH  - Sus scrofa/blood/*genetics/immunology
PMC - PMC8471458
OTO - NOTNLM
OT  - immune cells
OT  - immune-related genes
OT  - pig
OT  - single-nucleotide polymorphism
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/29 06:00
MHDA- 2022/02/15 06:00
PMCR- 2021/08/31
CRDT- 2021/09/28 01:08
PHST- 2021/07/26 00:00 [received]
PHST- 2021/08/25 00:00 [revised]
PHST- 2021/08/30 00:00 [accepted]
PHST- 2021/09/28 01:08 [entrez]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2022/02/15 06:00 [medline]
PHST- 2021/08/31 00:00 [pmc-release]
AID - genes12091377 [pii]
AID - genes-12-01377 [pii]
AID - 10.3390/genes12091377 [doi]
PST - epublish
SO  - Genes (Basel). 2021 Aug 31;12(9):1377. doi: 10.3390/genes12091377.