PMID- 34575028
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240403
IS  - 2075-1729 (Print)
IS  - 2075-1729 (Electronic)
IS  - 2075-1729 (Linking)
VI  - 11
IP  - 9
DP  - 2021 Aug 26
TI  - Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated 
      in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach.
LID - 10.3390/life11090879 [doi]
LID - 879
AB  - Acellular amniotic membrane (AM) has been studied, with promising results on the 
      reconstruction of lesioned tissues, and has become an attractive approach for 
      tracheal repair. This study aimed to evaluate the repair of the trachea with 
      human umbilical cord mesenchymal stem cells (hucMSCs) differentiated in 
      chondrocytes, grown on an experimental model. Tracheal defects were induced by 
      surgical tracheostomy in 30 New Zealand rabbits, and the acellular amniotic 
      membrane, with or without cells, was covering the defect. The hucMSCs were 
      isolated and cultivated with chondrogenic differentiation over the culture of 14 
      days, and then grown on the AM. In this study, the AM was biocompatible and 
      hucMSCs differentiated into chondrocytes. Our results demonstrated an important 
      role for AM with cultured cells in the promotion of immature collagen, known to 
      produce tissue regeneration. In addition, cartilaginous tissue was found at the 
      tracheal defects, demonstrated by immunohistology results. This study suggests 
      that this biomaterial implantation can be an effective future therapeutic 
      alternative for patients with tracheal injury.
FAU - Baggio Simeoni, Paulo Ricardo
AU  - Baggio Simeoni PR
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
FAU - Simeoni, Rossana Baggio
AU  - Simeoni RB
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
FAU - Bispo Machado Junior, Paulo Andre
AU  - Bispo Machado Junior PA
AUID- ORCID: 0000-0003-1577-9195
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
FAU - de Almeida, Meila Bastos
AU  - de Almeida MB
AD  - Department of Veterinary Medicine, Universidade Federal do Parana (UFPR), Rua XV 
      de Novembro, 1299, Curitiba 80060-000, Parana, Brazil.
FAU - Dziedzic, Dilcele Silva Moreira
AU  - Dziedzic DSM
AUID- ORCID: 0000-0002-2582-137X
AD  - Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Research 
      Group, Pele Pequeno Principe Research Institute & Pequeno Principe Faculties 
      (FPP) Ave., Silva Jardim, 1632, Curitiba 80240-020, Parana, Brazil.
FAU - da Rosa, Nadia Nascimento
AU  - da Rosa NN
AUID- ORCID: 0000-0001-7933-5417
AD  - Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Research 
      Group, Pele Pequeno Principe Research Institute & Pequeno Principe Faculties 
      (FPP) Ave., Silva Jardim, 1632, Curitiba 80240-020, Parana, Brazil.
FAU - Ferreira Stricker, Priscila E
AU  - Ferreira Stricker PE
AD  - Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Research 
      Group, Pele Pequeno Principe Research Institute & Pequeno Principe Faculties 
      (FPP) Ave., Silva Jardim, 1632, Curitiba 80240-020, Parana, Brazil.
FAU - Dos Santos Miggiolaro, Anna Flavia Ribeiro
AU  - Dos Santos Miggiolaro AFR
AUID- ORCID: 0000-0003-0310-7164
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
FAU - Naves, Guilherme
AU  - Naves G
AUID- ORCID: 0000-0003-1970-6399
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
FAU - Neto, Nelson Bergonse
AU  - Neto NB
AUID- ORCID: 0000-0003-4860-2855
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
FAU - de Noronha, Lucia
AU  - de Noronha L
AUID- ORCID: 0000-0003-2781-9705
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
FAU - Francisco, Julio Cesar
AU  - Francisco JC
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
FAU - Teixeira de Carvalho, Katherine Athayde
AU  - Teixeira de Carvalho KA
AD  - Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Research 
      Group, Pele Pequeno Principe Research Institute & Pequeno Principe Faculties 
      (FPP) Ave., Silva Jardim, 1632, Curitiba 80240-020, Parana, Brazil.
FAU - Guarita-Souza, Luiz Cesar
AU  - Guarita-Souza LC
AD  - Experimental Laboratory of Institute of Biological and Health Sciences of 
      Pontifical, Catholic University of Parana (PUCPR), Street Imaculada Conceicao, 
      1155, Curitiba 80215-901, Parana, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20210826
PL  - Switzerland
TA  - Life (Basel)
JT  - Life (Basel, Switzerland)
JID - 101580444
PMC - PMC8466253
OTO - NOTNLM
OT  - acellular amniotic membrane
OT  - chondrocytes
OT  - human umbilical cord mesenchymal stem cells
OT  - tissue engineering
OT  - tracheostomy
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/29 06:00
MHDA- 2021/09/29 06:01
PMCR- 2021/08/26
CRDT- 2021/09/28 01:14
PHST- 2021/07/20 00:00 [received]
PHST- 2021/08/12 00:00 [revised]
PHST- 2021/08/13 00:00 [accepted]
PHST- 2021/09/28 01:14 [entrez]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2021/09/29 06:01 [medline]
PHST- 2021/08/26 00:00 [pmc-release]
AID - life11090879 [pii]
AID - life-11-00879 [pii]
AID - 10.3390/life11090879 [doi]
PST - epublish
SO  - Life (Basel). 2021 Aug 26;11(9):879. doi: 10.3390/life11090879.