PMID- 34576362 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20211001 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 14 IP - 18 DP - 2021 Sep 7 TI - Improvement and Soil Consistency of Sand-Clay Mixtures Treated with Enzymatic-Induced Carbonate Precipitation. LID - 10.3390/ma14185140 [doi] LID - 5140 AB - Recently, microbially induced carbonate precipitation (MICP) has been studied as an alternative for the improvement of sand-clay mixtures. However, the cementing uniformity of MICP-treated sand-clay mixtures cannot be guaranteed. In this present study, enzymatic-induced carbonate precipitation (EICP) was used to deal with it. The ions used in kaolin clay was predicted to affect the production rate for calcium carbonate (CaCO(3)), which was studied using the calcification test. The solidification test was conducted using two different methods (the premixing method and the diffusion method). The permeability, unconfined compressive strength and the content of CaCO(3) of treated samples were obtained to evaluate the solidification effect of the EICP method. Moreover, in EICP treatment, the particle aggregation decreased the liquid limit, but the addition of solution increased it. Therefore, there were contrary effects to the soil consistency. In this study, the two types of liquid limits of treated samples were measured with deionized water and 2M-NaCl brine, respectively. The results show that the Al(2)O(3), NaCl and MgCl(2) in the kaolin clay had a slight impact on the production rate for CaCO(3), while FeCl(3) significantly inhibited it. The EICP method can improve sand-clay mixtures and decrease their permeability. Different from MICP, the EICP method can guarantee the uniformity of treated samples. Moreover, the liquid limit of the sample treated with the premixing method decreased, while that of the sample treated with the diffusion method increased firstly and then decreased with the increasing treatment cycles. Different from the deionized water, the pore-fluid chemistry had a larger effect on the liquid limit with 2M-NaCl brine. FAU - Mo, Yixin AU - Mo Y AUID- ORCID: 0000-0002-0199-089X AD - College of Defence Engineering, Army Engineering University of PLA, Nanjing 210007, China. FAU - Yue, Songlin AU - Yue S AD - College of Defence Engineering, Army Engineering University of PLA, Nanjing 210007, China. FAU - Zhou, Qizhen AU - Zhou Q AUID- ORCID: 0000-0001-5509-0902 AD - College of Defence Engineering, Army Engineering University of PLA, Nanjing 210007, China. FAU - Liu, Xiao AU - Liu X AD - College of Defence Engineering, Army Engineering University of PLA, Nanjing 210007, China. LA - eng GR - 51808553/National Natural Science Foundation of China/ PT - Journal Article DEP - 20210907 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC8470128 OTO - NOTNLM OT - EICP OT - UCS OT - liquid limit OT - permeability OT - sand-clay mixtures COIS- The authors declare that they have no conflict of interest. EDAT- 2021/09/29 06:00 MHDA- 2021/09/29 06:01 PMCR- 2021/09/07 CRDT- 2021/09/28 01:19 PHST- 2021/07/20 00:00 [received] PHST- 2021/08/31 00:00 [revised] PHST- 2021/09/01 00:00 [accepted] PHST- 2021/09/28 01:19 [entrez] PHST- 2021/09/29 06:00 [pubmed] PHST- 2021/09/29 06:01 [medline] PHST- 2021/09/07 00:00 [pmc-release] AID - ma14185140 [pii] AID - materials-14-05140 [pii] AID - 10.3390/ma14185140 [doi] PST - epublish SO - Materials (Basel). 2021 Sep 7;14(18):5140. doi: 10.3390/ma14185140.