PMID- 34578069
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210930
IS  - 2073-4360 (Electronic)
IS  - 2073-4360 (Linking)
VI  - 13
IP  - 18
DP  - 2021 Sep 18
TI  - Antitumor Activity of Rosmarinic Acid-Loaded Silk Fibroin Nanoparticles on HeLa 
      and MCF-7 Cells.
LID - 10.3390/polym13183169 [doi]
LID - 3169
AB  - Rosmarinic acid (RA), one of the most important polyphenol-based antioxidants, 
      has drawn increasing attention because of its remarkable bioactive properties, 
      including anti-inflammatory, anticancer and antibacterial activities. The aim of 
      this study was to synthesize and characterize RA-loaded silk fibroin 
      nanoparticles (RA-SFNs) in terms of their physical-chemical features and 
      composition, and to investigate their antitumor activity against human cervical 
      carcinoma and breast cancer cell lines (HeLa and MCF-7). Compared with the free 
      form, RA bioavailability was enhanced when the drug was adsorbed onto the surface 
      of the silk fibroin nanoparticles (SFNs). The resulting particle diameter was 255 
      nm, with a polydispersity index of 0.187, and the Z-potential was -17 mV. The 
      drug loading content of the RA-SFNs was 9.4 wt.%. Evaluation of the in vitro drug 
      release of RA from RA-SFNs pointed to a rapid release in physiological conditions 
      (50% of the total drug content was released in 0.5 h). Unloaded SFNs exhibited 
      good biocompatibility, with no significant cytotoxicity observed during the first 
      48 h against HeLa and MCF-7 cancer cells. In contrast, cell death increased in a 
      concentration-dependent manner after treatment with RA-SFNs, reaching an IC(50) 
      value of 1.568 and 1.377 mg/mL on HeLa and MCF-7, respectively. For both cell 
      lines, the IC(50) of free RA was higher. The cellular uptake of the nanoparticles 
      studied was increased when RA was loaded on them. The cell cycle and apoptosis 
      studies revealed that RA-SFNs inhibit cell proliferation and induce apoptosis on 
      HeLa and MCF-7 cell lines. It is concluded, therefore, that the RA delivery 
      platform based on SFNs improves the antitumor potential of RA in the case of the 
      above cancers.
FAU - Fuster, Marta G
AU  - Fuster MG
AD  - Chemical Engineering Department, Faculty of Chemistry, Regional Campus of 
      International Excellence "Campus Mare Nostrum", University of Murcia, 30071 
      Murcia, Spain.
FAU - Carissimi, Guzman
AU  - Carissimi G
AUID- ORCID: 0000-0001-8808-7654
AD  - Chemical Engineering Department, Faculty of Chemistry, Regional Campus of 
      International Excellence "Campus Mare Nostrum", University of Murcia, 30071 
      Murcia, Spain.
FAU - Montalban, Mercedes G
AU  - Montalban MG
AUID- ORCID: 0000-0003-1085-3042
AD  - Chemical Engineering Department, Faculty of Chemistry, Regional Campus of 
      International Excellence "Campus Mare Nostrum", University of Murcia, 30071 
      Murcia, Spain.
FAU - Villora, Gloria
AU  - Villora G
AUID- ORCID: 0000-0002-7325-9131
AD  - Chemical Engineering Department, Faculty of Chemistry, Regional Campus of 
      International Excellence "Campus Mare Nostrum", University of Murcia, 30071 
      Murcia, Spain.
LA  - eng
GR  - RG2020-002UM/University of Murcia/
GR  - CTQ2017-87708-R/Ministerio de Economia y Competitividad/
GR  - PID2020-113081RB-I00/Ministerio de Ciencia e Innovacion/
GR  - 20977/PI/18/Fundacion Seneca/
GR  - PRE2018-086441/Ministerio de Economia y Competitividad/
PT  - Journal Article
DEP - 20210918
PL  - Switzerland
TA  - Polymers (Basel)
JT  - Polymers
JID - 101545357
PMC - PMC8467615
OTO - NOTNLM
OT  - antitumor activity
OT  - apoptosis
OT  - cell cycle
OT  - cell viability
OT  - cellular uptake
OT  - nanoparticle
OT  - rosmarinic acid
OT  - silk fibroin
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/29 06:00
MHDA- 2021/09/29 06:01
PMCR- 2021/09/18
CRDT- 2021/09/28 01:25
PHST- 2021/08/29 00:00 [received]
PHST- 2021/09/15 00:00 [revised]
PHST- 2021/09/16 00:00 [accepted]
PHST- 2021/09/28 01:25 [entrez]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2021/09/29 06:01 [medline]
PHST- 2021/09/18 00:00 [pmc-release]
AID - polym13183169 [pii]
AID - polymers-13-03169 [pii]
AID - 10.3390/polym13183169 [doi]
PST - epublish
SO  - Polymers (Basel). 2021 Sep 18;13(18):3169. doi: 10.3390/polym13183169.