PMID- 34578436
OWN - NLM
STAT- MEDLINE
DCOM- 20211014
LR  - 20211014
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 13
IP  - 9
DP  - 2021 Sep 17
TI  - Increased sHLA-G Is Associated with Improved COVID-19 Outcome and Reduced 
      Neutrophil Adhesion.
LID - 10.3390/v13091855 [doi]
LID - 1855
AB  - Human leukocyte antigen (HLA) is a group of molecules involved in inflammatory 
      and infective responses. We evaluated blood sHLA-E and sHLA-G levels in 
      hospitalized COVID-19 patients with respiratory failure and their relationship 
      with clinical evolution, changes in endothelial activation biomarker profile, and 
      neutrophil adhesion. sHLA-E, sHLA-G, and endothelial activation biomarkers were 
      quantified by ELISA assay in plasma samples. Neutrophil adhesion to endothelium 
      was assessed in the presence/absence of patients' plasma samples. At admission, 
      plasma levels of sHLA-G and sHLA-E were significantly higher in COVID-19 patients 
      with respiratory failure compared to controls. COVID-19 clinical improvement was 
      associated with increased sHLA-G plasma levels. In COVID-19, but not in control 
      patients, an inverse correlation was found between serum sICAM-1 and E-selectin 
      levels and plasma sHLA-G values. The in vitro analysis of activated endothelial 
      cells confirmed the ability of HLA-G molecules to control sICAM-1 and sE-selectin 
      expression via CD160 interaction and FGF2 induction and consequently neutrophil 
      adhesion. We suggest a potential role for sHLA-G in improving COVID-19 patients' 
      clinical condition related to the control of neutrophil adhesion to activated 
      endothelium.
FAU - Bortolotti, Daria
AU  - Bortolotti D
AUID- ORCID: 0000-0003-4496-9561
AD  - Department of Chemical, Pharmaceutical and Agricultural Science, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Gentili, Valentina
AU  - Gentili V
AUID- ORCID: 0000-0003-4572-5497
AD  - Department of Chemical, Pharmaceutical and Agricultural Science, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Rizzo, Sabrina
AU  - Rizzo S
AD  - Department of Chemical, Pharmaceutical and Agricultural Science, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Schiuma, Giovanna
AU  - Schiuma G
AUID- ORCID: 0000-0003-4652-5550
AD  - Department of Chemical, Pharmaceutical and Agricultural Science, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Beltrami, Silvia
AU  - Beltrami S
AUID- ORCID: 0000-0002-5923-9265
AD  - Department of Chemical, Pharmaceutical and Agricultural Science, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Spadaro, Savino
AU  - Spadaro S
AUID- ORCID: 0000-0001-5027-5318
AD  - Intensive Care Unit, Department of Translational Medicine, University of Ferrara, 
      44121 Ferrara, Italy.
FAU - Strazzabosco, Giovanni
AU  - Strazzabosco G
AD  - Department of Chemical, Pharmaceutical and Agricultural Science, University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Campo, Gianluca
AU  - Campo G
AD  - Cardiology Unit, Azienda Ospedaliero Universitaria di Ferrara, Cona, 44124 
      Ferrara, Italy.
FAU - Carosella, Edgardo D
AU  - Carosella ED
AD  - CEA, Institute of Emerging Diseases and Innovative Therapies (iMETI), Research 
      Division in Hematology and Immunology (SRHI), Saint-Louis Hospital, 75001 Paris, 
      France.
FAU - Papi, Alberto
AU  - Papi A
AD  - Respiratory Section, Department of Translational Medicine, University of Ferrara, 
      44121 Ferrara, Italy.
AD  - Respiratory Unit, Azienda Ospedaliera Universitaria Ferrara, Cona, 44124 Ferrara, 
      Italy.
FAU - Rizzo, Roberta
AU  - Rizzo R
AUID- ORCID: 0000-0001-9507-9126
AD  - Department of Chemical, Pharmaceutical and Agricultural Science, University of 
      Ferrara, 44121 Ferrara, Italy.
AD  - Industrial Research and Technology Transfer Laboratory (LTTA), University of 
      Ferrara, 44121 Ferrara, Italy.
FAU - Contoli, Marco
AU  - Contoli M
AUID- ORCID: 0000-0002-2731-5809
AD  - Respiratory Section, Department of Translational Medicine, University of Ferrara, 
      44121 Ferrara, Italy.
AD  - Respiratory Unit, Azienda Ospedaliera Universitaria Ferrara, Cona, 44124 Ferrara, 
      Italy.
LA  - eng
GR  - Crowfounding 2021/Universita degli Studi di Ferrara/
GR  - 5x1000 2021/Universita di Ferrara/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210917
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Biomarkers)
RN  - 0 (HLA-G Antigens)
SB  - IM
MH  - Aged
MH  - Alleles
MH  - *Biomarkers
MH  - COVID-19/epidemiology/*immunology/*virology
MH  - Cell Adhesion/immunology
MH  - Endothelial Cells/immunology/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Frequency
MH  - HLA-G Antigens/blood/*immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Models, Biological
MH  - Neutrophils/*immunology/metabolism
MH  - SARS-CoV-2/*immunology
PMC - PMC8473385
OTO - NOTNLM
OT  - CD160
OT  - COVID-19
OT  - E-selectin
OT  - HLA-G
OT  - ICAM-1
OT  - neutrophil
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/29 06:00
MHDA- 2021/10/15 06:00
PMCR- 2021/09/17
CRDT- 2021/09/28 01:26
PHST- 2021/08/13 00:00 [received]
PHST- 2021/09/12 00:00 [revised]
PHST- 2021/09/14 00:00 [accepted]
PHST- 2021/09/28 01:26 [entrez]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2021/10/15 06:00 [medline]
PHST- 2021/09/17 00:00 [pmc-release]
AID - v13091855 [pii]
AID - viruses-13-01855 [pii]
AID - 10.3390/v13091855 [doi]
PST - epublish
SO  - Viruses. 2021 Sep 17;13(9):1855. doi: 10.3390/v13091855.