PMID- 34578477
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231107
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 11
IP  - 9
DP  - 2021 Aug 24
TI  - Tuning the Reduction of Graphene Oxide Nanoflakes Differently Affects Neuronal 
      Networks in the Zebrafish.
LID - 10.3390/nano11092161 [doi]
LID - 2161
AB  - The increasing engineering of biomedical devices and the design of drug-delivery 
      platforms enriched by graphene-based components demand careful investigations of 
      the impact of graphene-related materials (GRMs) on the nervous system. In 
      addition, the enhanced diffusion of GRM-based products and technologies that 
      might favor the dispersion in the environment of GRMs nanoparticles urgently 
      requires the potential neurotoxicity of these compounds to be addressed. One of 
      the challenges in providing definite evidence supporting the harmful or safe use 
      of GRMs is addressing the variety of this family of materials, with GRMs 
      differing for size and chemistry. Such a diversity impairs reaching a unique and 
      predictive picture of the effects of GRMs on the nervous system. Here, by 
      exploiting the thermal reduction of graphene oxide nanoflakes (GO) to generate 
      materials with different oxygen/carbon ratios, we used a high-throughput analysis 
      of early-stage zebrafish locomotor behavior to investigate if modifications of a 
      specific GRM chemical property influenced how these nanomaterials affect 
      vertebrate sensory-motor neurophysiology-exposing zebrafish to GO downregulated 
      their swimming performance. Conversely, reduced GO (rGO) treatments boosted 
      locomotor activity. We concluded that the tuning of single GRM chemical 
      properties is sufficient to produce differential effects on nervous system 
      physiology, likely interfering with different signaling pathways.
FAU - Di Mauro, Giuseppe
AU  - Di Mauro G
AD  - Neuron Physiology and Technology Lab, International School for Advanced Studies 
      (SISSA), Neuroscience, Via Bonomea 265, 34136 Trieste, Italy.
FAU - Rauti, Rossana
AU  - Rauti R
AD  - Neuron Physiology and Technology Lab, International School for Advanced Studies 
      (SISSA), Neuroscience, Via Bonomea 265, 34136 Trieste, Italy.
FAU - Casani, Raffaele
AU  - Casani R
AD  - Neuron Physiology and Technology Lab, International School for Advanced Studies 
      (SISSA), Neuroscience, Via Bonomea 265, 34136 Trieste, Italy.
FAU - Chimowa, George
AU  - Chimowa G
AUID- ORCID: 0000-0002-7421-1068
AD  - CIRIMAT, UMR CNRS 5085, Universite Toulouse Paul Sabatier, Bat. CIRIMAT, 118 
      Route de Narbonne, CEDEX 9, 31062 Toulouse, France.
FAU - Galibert, Anne Marie
AU  - Galibert AM
AD  - CIRIMAT, UMR CNRS 5085, Universite Toulouse Paul Sabatier, Bat. CIRIMAT, 118 
      Route de Narbonne, CEDEX 9, 31062 Toulouse, France.
FAU - Flahaut, Emmanuel
AU  - Flahaut E
AUID- ORCID: 0000-0001-8344-6902
AD  - CIRIMAT, UMR CNRS 5085, Universite Toulouse Paul Sabatier, Bat. CIRIMAT, 118 
      Route de Narbonne, CEDEX 9, 31062 Toulouse, France.
FAU - Cellot, Giada
AU  - Cellot G
AUID- ORCID: 0000-0001-9198-8402
AD  - Neuron Physiology and Technology Lab, International School for Advanced Studies 
      (SISSA), Neuroscience, Via Bonomea 265, 34136 Trieste, Italy.
FAU - Ballerini, Laura
AU  - Ballerini L
AUID- ORCID: 0000-0001-8420-0787
AD  - Neuron Physiology and Technology Lab, International School for Advanced Studies 
      (SISSA), Neuroscience, Via Bonomea 265, 34136 Trieste, Italy.
LA  - eng
GR  - 881603/Horizon 2020/
PT  - Journal Article
DEP - 20210824
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC8468975
OTO - NOTNLM
OT  - graphene oxide
OT  - reduced graphene oxide
OT  - sensory-motor nervous system
OT  - synapses
OT  - zebrafish larvae
COIS- The authors declare no conflict of interest.
EDAT- 2021/09/29 06:00
MHDA- 2021/09/29 06:01
PMCR- 2021/08/24
CRDT- 2021/09/28 01:26
PHST- 2021/06/28 00:00 [received]
PHST- 2021/08/18 00:00 [revised]
PHST- 2021/08/20 00:00 [accepted]
PHST- 2021/09/28 01:26 [entrez]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2021/09/29 06:01 [medline]
PHST- 2021/08/24 00:00 [pmc-release]
AID - nano11092161 [pii]
AID - nanomaterials-11-02161 [pii]
AID - 10.3390/nano11092161 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2021 Aug 24;11(9):2161. doi: 10.3390/nano11092161.