PMID- 34580847
OWN - NLM
STAT- MEDLINE
DCOM- 20220113
LR  - 20220113
IS  - 1897-5631 (Electronic)
IS  - 0239-8508 (Linking)
VI  - 59
IP  - 3
DP  - 2021
TI  - SDF-1/CXCR4 axis promotes osteogenic differentiation of BMSCs through the 
      JAK2/STAT3 pathway.
PG  - 187-194
LID - 10.5603/FHC.a2021.0020 [doi]
AB  - INTRODUCTION: This study aimed to investigate the effects of stromal cell-derived 
      factor-1 (SDF-1) and activation of its receptor, chemokine receptor 4 (CXCR4), on 
      the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and 
      the key signaling mechanisms involved in these effects. MATERIAL AND METHODS: 
      BMSCs were treated with 100 mug/L SDF-1 and cultured in osteogenic medium for 7 
      days. RT-qPCR and western blotting were used to detect the protein and mRNA 
      levels of Janus kinase 2 (JAK2), signal transducer and activator of transcription 
      3 (STAT3), Runt-related transcription factor 2 (Runx2), and osteocalcin (OCN). 
      Alizarin-red staining was used to detect the mineralization-inducing ability of 
      the cells. RESULTS: After BMSCs were treated with SDF-1, the levels of JAK2 mRNA, 
      STAT3 mRNA, and protein phosphorylation increased, the number of mineralized 
      nodules of BMSCs increased, and the osteogenic-differentiation ability was 
      enhanced. In addition, after BMSCs were treated with an inhibitor of JAK2 
      phosphorylation, the levels of JAK2, STAT3, Runx2, and OCN decreased 
      significantly, the number of mineralized nodules of BMSCs also decreased, and the 
      osteogenic-differentiation ability decreased. The inhibition of CXCR4-treated 
      BMSCs further confirmed that SDF-1/CXCR4 activated JAK2/STAT3 to regulate the 
      osteogenic differentiation of BMSCs. CONCLUSIONS: SDF-1/CXCR4 promoted the 
      osteogenic differentiation of BMSCs through JAK2/STAT3 activation.
FAU - Xiong, Wen
AU  - Xiong W
AD  - The First School of Clinical Medicine, Southern Medical University, Guangdong, 
      China.
FAU - Guo, Xin
AU  - Guo X
AD  - The First School of Clinical Medicine, Southern Medical University, Guangdong, 
      China.
FAU - Cai, Xianhua
AU  - Cai X
AD  - The First School of Clinical Medicine, Southern Medical University, Guangdong, 
      China. whcaixh@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210928
PL  - Poland
TA  - Folia Histochem Cytobiol
JT  - Folia histochemica et cytobiologica
JID - 8502651
RN  - 0 (STAT3 Transcription Factor)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - Cell Differentiation
MH  - Janus Kinase 2/metabolism
MH  - *Mesenchymal Stem Cells/metabolism
MH  - *Osteogenesis
MH  - STAT3 Transcription Factor/metabolism
OTO - NOTNLM
OT  - BMSCs
OT  - CXCR4
OT  - JAK2/STAT3
OT  - Runx2
OT  - SDF-1
OT  - alizarin red
OT  - osteocalcin
OT  - osteogenic differentiation
EDAT- 2021/09/29 06:00
MHDA- 2022/01/14 06:00
CRDT- 2021/09/28 06:50
PHST- 2021/05/18 00:00 [received]
PHST- 2021/09/24 00:00 [accepted]
PHST- 2021/09/23 00:00 [revised]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2022/01/14 06:00 [medline]
PHST- 2021/09/28 06:50 [entrez]
AID - VM/OJS/J/83368 [pii]
AID - 10.5603/FHC.a2021.0020 [doi]
PST - ppublish
SO  - Folia Histochem Cytobiol. 2021;59(3):187-194. doi: 10.5603/FHC.a2021.0020. Epub 
      2021 Sep 28.