PMID- 34581594
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211028
IS  - 2379-5077 (Print)
IS  - 2379-5077 (Electronic)
IS  - 2379-5077 (Linking)
VI  - 6
IP  - 5
DP  - 2021 Oct 26
TI  - Lineage-Specific Growth Curves Document Large Differences in Response of 
      Individual Groups of Marine Bacteria to the Top-Down and Bottom-Up Controls.
PG  - e0093421
LID - 10.1128/mSystems.00934-21 [doi]
LID - e00934-21
AB  - Marine bacterioplankton represent a diverse assembly of species differing largely 
      in their abundance, physiology, metabolic activity, and role in microbial food 
      webs. To analyze their sensitivity to bottom-up and top-down controls, we 
      performed a manipulation experiment where grazers were removed, with or without 
      the addition of phosphate. Using amplicon-reads normalization by internal 
      standard (ARNIS), we reconstructed growth curves for almost 300 individual 
      phylotypes. Grazer removal caused a rapid growth of most bacterial groups, which 
      grew at rates of 0.6 to 3.5 day(-1), with the highest rates (>4 day(-1)) recorded 
      among Rhodobacteraceae, Oceanospirillales, Alteromonadaceae, and Arcobacteraceae. 
      Based on their growth response, the phylotypes were divided into three basic 
      groups. Most of the phylotypes responded positively to both grazer removal as 
      well as phosphate addition. The second group (containing, e.g., Rhodobacterales 
      and Rhizobiales) responded to the grazer removal but not to the phosphate 
      addition. Finally, some clades, such as SAR11 and Flavobacteriaceae, responded 
      only to phosphate amendment but not to grazer removal. Our results show large 
      differences in bacterial responses to experimental manipulations at the phylotype 
      level and document different life strategies of marine bacterioplankton. In 
      addition, growth curves of 130 phylogroups of aerobic anoxygenic phototrophs were 
      reconstructed based on changes of the functional pufM gene. The use of functional 
      genes together with rRNA genes may significantly expand the scientific potential 
      of the ARNIS technique. IMPORTANCE Growth is one of the main manifestations of 
      life. It is assumed generally that bacterial growth is constrained mostly by 
      nutrient availability (bottom-up control) and grazing (top-down control). Since 
      marine bacteria represent a very diverse assembly of species with different 
      metabolic properties, their growth characteristics also largely differ 
      accordingly. Currently, the growth of marine microorganisms is typically 
      evaluated using microscopy in combination with fluorescence in situ hybridization 
      (FISH). However, these laborious techniques are limited in their throughput and 
      taxonomical resolution. Therefore, we combined a classical manipulation 
      experiment with next-generation sequencing to resolve the growth dynamics of 
      almost 300 bacterial phylogroups in the coastal Adriatic Sea. The analysis 
      documented that most of the phylogroups responded positively to both grazer 
      removal and phosphate addition. We observed significant differences in growth 
      kinetics among closely related species, which could not be distinguished by the 
      classical FISH technique.
FAU - Fecskeova, Livia K
AU  - Fecskeova LK
AD  - Center Algatech, Institute of Microbiology of the Czech Academy of Sciences, 
      Trebon, Czechia.
FAU - Piwosz, Kasia
AU  - Piwosz K
AUID- ORCID: 0000-0002-3248-3364
AD  - Center Algatech, Institute of Microbiology of the Czech Academy of Sciences, 
      Trebon, Czechia.
AD  - National Marine Fisheries Research Institute, Gdynia, Poland.
FAU - Santic, Danijela
AU  - Santic D
AD  - Laboratory of Marine Microbiology, Institute of Oceanography and 
      Fisheriesgrid.425052.4, Split, Croatia.
FAU - Sestanovic, Stefanija
AU  - Sestanovic S
AD  - Laboratory of Marine Microbiology, Institute of Oceanography and 
      Fisheriesgrid.425052.4, Split, Croatia.
FAU - Tomas, Ana Vrdoljak
AU  - Tomas AV
AD  - Laboratory of Marine Microbiology, Institute of Oceanography and 
      Fisheriesgrid.425052.4, Split, Croatia.
FAU - Hanusova, Martina
AU  - Hanusova M
AD  - Center Algatech, Institute of Microbiology of the Czech Academy of Sciences, 
      Trebon, Czechia.
FAU - Solic, Mladen
AU  - Solic M
AD  - Laboratory of Marine Microbiology, Institute of Oceanography and 
      Fisheriesgrid.425052.4, Split, Croatia.
FAU - Koblizek, Michal
AU  - Koblizek M
AUID- ORCID: 0000-0001-6938-2340
AD  - Center Algatech, Institute of Microbiology of the Czech Academy of Sciences, 
      Trebon, Czechia.
LA  - eng
GR  - 18-14095Y/Grantova Agentura Ceske Republiky (GACR)/
GR  - UIP-2019-04-8401/Hrvatska Zaklada za Znanost (HRZZ)/
PT  - Journal Article
DEP - 20210928
PL  - United States
TA  - mSystems
JT  - mSystems
JID - 101680636
PMC - PMC8547455
OTO - NOTNLM
OT  - aerobic anoxygenic phototrophs
OT  - amplicon sequencing
OT  - bacterioplankton
OT  - grazing
OT  - growth curves
OT  - growth rate
OT  - manipulation experiment
OT  - phosphorus limitation
OT  - top-down control
EDAT- 2021/09/29 06:00
MHDA- 2021/09/29 06:01
PMCR- 2021/09/28
CRDT- 2021/09/28 12:12
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2021/09/29 06:01 [medline]
PHST- 2021/09/28 12:12 [entrez]
PHST- 2021/09/28 00:00 [pmc-release]
AID - mSystems00934-21 [pii]
AID - msystems.00934-21 [pii]
AID - 10.1128/mSystems.00934-21 [doi]
PST - ppublish
SO  - mSystems. 2021 Oct 26;6(5):e0093421. doi: 10.1128/mSystems.00934-21. Epub 2021 
      Sep 28.