PMID- 34582049
OWN - NLM
STAT- MEDLINE
DCOM- 20220210
LR  - 20220210
IS  - 1097-0223 (Electronic)
IS  - 0197-3851 (Linking)
VI  - 41
IP  - 13
DP  - 2021 Dec
TI  - Identification of fetal aneuploidy with dual-probe fluorescence in situ 
      hybridization analysis in circulating trophoblasts after enrichment using a 
      high-sensitivity microfluidic platform.
PG  - 1701-1708
LID - 10.1002/pd.6046 [doi]
AB  - OBJECTIVE: To evaluate a microfluidics-based positive selection technology for 
      isolating circulating trophoblasts (CTs) from peripheral blood of women whose 
      pregnancies are affected by aneuploidy and to evaluate fetal karyotype using 
      fluorescence in situ hybridization (FISH). METHOD: Ten 18-ml samples of 
      peripheral blood were collected consecutively from pregnant women whose fetus was 
      affected by aneuploidy. A preservation buffer was added, and the specimens were 
      shipped overnight to the testing laboratory at ambient temperature. The specimen 
      was infused into the fully automated microfluidics-based LiquidScan((R)) instrument 
      without pre-processing. This instrument contains microfluidic chips, which are 
      coated with antibodies (anti-huEpCAM and a proprietary antibody mixture) specific 
      to CT surface epitopes. FISH analysis was performed on the enriched cells. 
      RESULTS: Fetal aneuploidy evaluated included trisomy 21 (n = 3), trisomy 18 
      (n = 1), trisomy 13 (n = 1), monosomy X (n = 3), and triploidy (n = 1). CTs for 
      analysis by FISH were identified in all samples. The average number of 
      mononucleate cells per 1 ml of whole blood was 2.11 (range 0.38-4.63) overall and 
      was 2.67 (range 1.13-4.63) using the proprietary combination of antibodies. FISH 
      results were concordant with the aneuploidy based on other testing in all cases. 
      Multinucleate cells were searched for and identified in the last seven samples 
      (average number: 0.84/1 ml). CONCLUSIONS: Our study demonstrates that the 
      LiquidScan((R)) , a high-sensitivity microfluidic platform, can enrich circulating 
      trophoblasts (mononucleate and multinucleate). FISH can then be used to detect 
      fetal aneuploidy.
CI  - (c) 2021 John Wiley & Sons Ltd.
FAU - Sonek, Jiri
AU  - Sonek J
AUID- ORCID: 0000-0002-9167-1170
AD  - Department of Obstetrics and Gynecology, Wright State University, Dayton, Ohio, 
      USA.
FAU - Muller, Rolf
AU  - Muller R
AD  - BioFluidica, Inc., San Diego, California, USA.
FAU - Muller-Cohn, Judy
AU  - Muller-Cohn J
AD  - BioFluidica, Inc., San Diego, California, USA.
FAU - Dickerson, Janet
AU  - Dickerson J
AD  - BioFluidica, Inc., San Diego, California, USA.
FAU - Garcia Lopez, Brenda
AU  - Garcia Lopez B
AD  - BioFluidica, Inc., San Diego, California, USA.
FAU - Barber-Singh, Jennifer
AU  - Barber-Singh J
AD  - BioFluidica, Inc., San Diego, California, USA.
FAU - Dufek, Dylan
AU  - Dufek D
AD  - BioFluidica, Inc., San Diego, California, USA.
FAU - Hiett, Adam Kinney
AU  - Hiett AK
AUID- ORCID: 0000-0003-1347-6131
AD  - Department of Obstetrics and Gynecology, Wright State University, Dayton, Ohio, 
      USA.
FAU - Buchanan, Philip
AU  - Buchanan P
AD  - Department of Obstetrics and Gynecology, The George Washington University, 
      Washington, DC, USA.
LA  - eng
GR  - U.S. Department of Health and Human Services/
GR  - NH/NIH HHS/United States
GR  - R43HD098942/Eunice Kennedy Shriver National Institute of Child Health and Human 
      Development/
GR  - NH/NIH HHS/United States
PT  - Journal Article
DEP - 20210928
PL  - England
TA  - Prenat Diagn
JT  - Prenatal diagnosis
JID - 8106540
SB  - IM
MH  - Adult
MH  - *Aneuploidy
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/instrumentation/*methods/statistics & 
      numerical data
MH  - Microfluidics/*methods/statistics & numerical data
MH  - Pregnancy
MH  - Prenatal Diagnosis/methods
MH  - Trophoblasts/pathology/*physiology
EDAT- 2021/09/29 06:00
MHDA- 2022/02/11 06:00
CRDT- 2021/09/28 12:30
PHST- 2021/08/08 00:00 [revised]
PHST- 2021/05/22 00:00 [received]
PHST- 2021/09/09 00:00 [accepted]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2022/02/11 06:00 [medline]
PHST- 2021/09/28 12:30 [entrez]
AID - 10.1002/pd.6046 [doi]
PST - ppublish
SO  - Prenat Diagn. 2021 Dec;41(13):1701-1708. doi: 10.1002/pd.6046. Epub 2021 Sep 28.