PMID- 34583153
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231221
IS  - 1879-2472 (Electronic)
IS  - 0049-3848 (Linking)
VI  - 207
DP  - 2021 Nov
TI  - Brain-derived extracellular vesicles mediated coagulopathy, inflammation and 
      apoptosis after sepsis.
PG  - 85-95
LID - S0049-3848(21)00466-7 [pii]
LID - 10.1016/j.thromres.2021.09.014 [doi]
AB  - INTRODUCTION: The activation of coagulation, inflammation and other pathways is 
      the basic response of the host to infection in sepsis, but this response also 
      causes damage to the host. Brain-derived extracellular vesicles (BDEVs) have been 
      reported to cause a hypercoagulable state that can rapidly develop into 
      consumptive coagulopathy, which is consistent with the pathophysiological process 
      of sepsis-induced coagulopathy. However, the role of BDEVs in sepsis-induced 
      coagulopathy remains unclear. MATERIALS AND METHODS: Male Sprague-Dawley (SD) 
      rats were used for sepsis modeling using cecal ligation puncture (CLP). Flow 
      cytometry was used to measure the levels of circulating BDEVs. Enzyme-linked 
      immunosorbent assay (ELISA) was used to measure the serum levels of plasminogen 
      activator inhibitor type 1 (PAI-1), thrombin-antithrombin (TAT), D-dimer, 
      fibrinogen(Fib), tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta and 
      IL-6. Nanoparticle tracking analysis (NTA) and Transmission electron microscopy 
      (TEM) were used to identify BDEVs. Western blot (WB) was used to determine the 
      expression of glial fibrillary acidic protein (GFAP), neuron-specific enolase 
      (NSE), bax, bcl-2 and cleaved caspase-3. Hematoxylin-eosin (HE) and terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining 
      were performed to detect tissue injury. Survival was monitored over the course of 
      168 h. RESULTS: We found that a large number of BDEVs were released into the 
      circulating blood in septic rats. Moreover, we observed that BDEVs injection 
      activated the systemic coagulation reaction and induced lung, liver and kidney 
      inflammation and apoptosis(P < .05). Compared with BDEVs from sham-operated rats, 
      BDEVs from septic rats exacerbated this process(P < .05). CONCLUSIONS: This 
      finding suggests that inhibiting BDEVs may yield therapeutic benefits in the 
      treatment of sepsis-induced coagulopathy.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Lin, Huaying
AU  - Lin H
AD  - Department of Anesthesia, Tianjin Medical University General Hospital, Tianjin 
      300052, China; Tianjin Institute of Anesthesiology, Tianjin Medical University 
      General Hospital, Tianjin 300052, China.
FAU - Chen, Hongguang
AU  - Chen H
AD  - Department of Anesthesia, Tianjin Medical University General Hospital, Tianjin 
      300052, China; Tianjin Institute of Anesthesiology, Tianjin Medical University 
      General Hospital, Tianjin 300052, China.
FAU - Qi, Bo
AU  - Qi B
AD  - Department of Anesthesia, Tianjin Medical University General Hospital, Tianjin 
      300052, China; Tianjin Institute of Anesthesiology, Tianjin Medical University 
      General Hospital, Tianjin 300052, China.
FAU - Jiang, Yi
AU  - Jiang Y
AD  - Department of Anesthesia, Tianjin Medical University General Hospital, Tianjin 
      300052, China; Tianjin Institute of Anesthesiology, Tianjin Medical University 
      General Hospital, Tianjin 300052, China.
FAU - Lian, Naqi
AU  - Lian N
AD  - Department of Anesthesia, Tianjin Medical University General Hospital, Tianjin 
      300052, China; Tianjin Institute of Anesthesiology, Tianjin Medical University 
      General Hospital, Tianjin 300052, China.
FAU - Zhuang, Xiaoli
AU  - Zhuang X
AD  - Department of Anesthesia, Tianjin Medical University General Hospital, Tianjin 
      300052, China; Tianjin Institute of Anesthesiology, Tianjin Medical University 
      General Hospital, Tianjin 300052, China.
FAU - Yu, Yonghao
AU  - Yu Y
AD  - Department of Anesthesia, Tianjin Medical University General Hospital, Tianjin 
      300052, China; Tianjin Institute of Anesthesiology, Tianjin Medical University 
      General Hospital, Tianjin 300052, China. Electronic address: yyu@tmu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20210923
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
SB  - IM
OTO - NOTNLM
OT  - Apoptosis
OT  - Coagulopathy
OT  - Extracellular vesicles
OT  - Inflammation
OT  - Sepsis
EDAT- 2021/09/29 06:00
MHDA- 2021/09/29 06:01
CRDT- 2021/09/28 20:22
PHST- 2021/06/04 00:00 [received]
PHST- 2021/09/14 00:00 [revised]
PHST- 2021/09/20 00:00 [accepted]
PHST- 2021/09/29 06:01 [medline]
PHST- 2021/09/29 06:00 [pubmed]
PHST- 2021/09/28 20:22 [entrez]
AID - S0049-3848(21)00466-7 [pii]
AID - 10.1016/j.thromres.2021.09.014 [doi]
PST - ppublish
SO  - Thromb Res. 2021 Nov;207:85-95. doi: 10.1016/j.thromres.2021.09.014. Epub 2021 
      Sep 23.