PMID- 34585589
OWN - NLM
STAT- MEDLINE
DCOM- 20211208
LR  - 20211214
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 68
IP  - 6
DP  - 2021 Nov
TI  - The matrix metalloproteinase gene family: a significant prognostic gene lineage 
      correlated with immune infiltrates in laryngeal squamous cell carcinoma.
PG  - 1212-1224
LID - 210511N643 [pii]
LID - 10.4149/neo_2021_210511N643 [doi]
AB  - This study aimed to elucidate the potential genes of the matrix metalloproteinase 
      (MMP) family, responsible for the progression of laryngeal squamous cell 
      carcinoma (LSCC). Besides, we ascertained the changes in common malignant 
      behaviors in vitro by knocking down MMP1. TCGA, GEO, Oncomine, and microarray 
      data were conducted to analyze the expression levels of MMPs and to find 
      tissue-specific genes in LSCC. Univariate and multivariate Cox regression 
      analyses were established in the construction of a prognostic model based on 
      expression profiles and clinical information of LSCC in TCGA. We then 
      comprehensively analyzed survival, co-expression network, and immune infiltration 
      based on a prognostic model by Kaplan-Meier analysis, WGCNA, and CIBERSORT. 
      Thereafter, qRT-PCR, proliferation, Transwell, and wound-healing assays were used 
      to assess the accuracy of the bioinformatics data. A total of seven genes in the 
      MMP family were identified as differentially expressed genes (DEGs) by 
      integrating three public databases and microarray data. Additionally, 
      multivariate Cox regression was used to establish a four-gene (MMP1/3/8/10) 
      prognostic model, which exhibited a better predictive accuracy than the TNM 
      (tumors/nodes/metastases) based model. The prognostic model was related to plasma 
      cells, CD8+ T cells, follicular helper T cells, resting NK cells, and M0 
      macrophages infiltration. The expression of MMP1, MMP3, and MMP10 was the highest 
      in head and neck squamous cell carcinoma (HNSC) compared to other cancer in the 
      Oncomine and GEPIA dataset. Further, MMP1 demonstrated significant upregulation 
      in 40 paired LSCC tissues. Eventually, MMP1 downregulation inhibited cell 
      viability, colony formation, and cell migration in TU686 and FaDu cells. Our 
      findings suggest that the four-gene signature might be associated with the 
      prognosis. Further, we revealed that MMP1 is a pivotal biomarker for the 
      biotherapy and prognostic evaluation of patients with LSCC.
FAU - Yang, Chuan
AU  - Yang C
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei, China.
FAU - Cao, Huan
AU  - Cao H
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei, China.
FAU - Yang, Jian-Wang
AU  - Yang JW
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei, China.
FAU - Liu, Tao
AU  - Liu T
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei, China.
FAU - Wang, Jian-Tao
AU  - Wang JT
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei, China.
FAU - Wang, Bao-Shan
AU  - Wang BS
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei, China.
LA  - eng
PT  - Journal Article
DEP - 20210928
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Biomarkers, Tumor/genetics
MH  - *Carcinoma, Squamous Cell/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - *Laryngeal Neoplasms/genetics
MH  - Prognosis
MH  - Squamous Cell Carcinoma of Head and Neck
EDAT- 2021/09/30 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/09/29 08:40
PHST- 2021/05/11 00:00 [received]
PHST- 2021/07/01 00:00 [accepted]
PHST- 2021/09/30 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/09/29 08:40 [entrez]
AID - 210511N643 [pii]
AID - 10.4149/neo_2021_210511N643 [doi]
PST - ppublish
SO  - Neoplasma. 2021 Nov;68(6):1212-1224. doi: 10.4149/neo_2021_210511N643. Epub 2021 
      Sep 28.