PMID- 34587984 OWN - NLM STAT- MEDLINE DCOM- 20211103 LR - 20240117 IS - 1532-429X (Electronic) IS - 1097-6647 (Print) IS - 1097-6647 (Linking) VI - 23 IP - 1 DP - 2021 Sep 30 TI - Quantification of myocardial hemorrhage using T2* cardiovascular magnetic resonance at 1.5T with ex-vivo validation. PG - 104 LID - 10.1186/s12968-021-00779-4 [doi] LID - 104 AB - BACKGROUND: T2* cardiovascular magnetic resonance (CMR) is commonly used in the diagnosis of intramyocardial hemorrhage (IMH). For quantifying IMH with T2* CMR, despite the lack of consensus studies, two different methods [subject-specific T2* (ssT2*) and absolute T2* thresholding (aT2* < 20 ms)] are interchangeably used. We examined whether these approaches yield equivalent information. METHODS: ST elevation myocardial infarction (STEMI) patients (n = 70) were prospectively recruited for CMR at 4-7 days post revascularization and for 6-month follow up (n = 43). Canines studies were performed for validation purposes, where animals (n = 20) were subject to reperfused myocardial infarction (MI) and those surviving the MI (n = 16) underwent CMR at 7 days and 8 weeks and then euthanized. Both in patients and animals, T2* of IMH and volume of IMH were determined using ssT2* and aT2* < 20 ms. In animals, ex-vivo T2* CMR and mass spectrometry for iron concentration ([Fe](Hemo)) were determined on excised myocardial sections. T2* values based on ssT2* and absolute T2* threshold approaches were independently regressed against [Fe](Hemo) and compared. A range of T2* cut-offs were tested to determine the optimized conditions relative to ssT2*. RESULTS: While both approaches showed many similarities, there were also differences. Compared to ssT2*, aT2* < 20 ms showed lower T2* and volume of IMH in patients and animals independent of MI age (all p < 0.005). While T2* determined from both methods were highly correlated against [Fe](Hemo) (R(2) = 0.9 for both), the slope of the regression curve for ssT2* was significantly larger as compared to aT2* < 20 ms (0.46 vs. 0.32, p < 0.01). Further, slightly larger absolute T2* cut-offs (patients: 23 ms; animals: 25 ms) showed similar IMH characteristics compared to ssT2*. CONCLUSION: Current quantification methods have excellent capacity to identify IMH, albeit the T2*of IMH and volume of IMH based on aT2* < 20 ms are smaller compared to ssT2*. Thus the method used to quantify IMH from T2* CMR may influence the diagnosis for IMH. CI - (c) 2021. The Author(s). FAU - Chen, Yinyin AU - Chen Y AD - Biomedical Imaging Research Institute, Dept of Biomedical Sciences, Cedars-Sinai Medical Center, Suite 400, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA. AD - Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. AD - Department of Medical Imaging, Shanghai Medical School, Fudan University and Shanghai Institute of Medical Imaging, Shanghai, 200032, China. FAU - Ren, Daoyuan AU - Ren D AD - Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai, China. FAU - Guan, Xingmin AU - Guan X AD - Biomedical Imaging Research Institute, Dept of Biomedical Sciences, Cedars-Sinai Medical Center, Suite 400, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA. AD - Department of Bioengineering, University of California at Los Angeles, Los Angeles, CA, USA. FAU - Yang, Hsin-Jung AU - Yang HJ AD - Biomedical Imaging Research Institute, Dept of Biomedical Sciences, Cedars-Sinai Medical Center, Suite 400, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA. FAU - Liu, Ting AU - Liu T AD - Biomedical Imaging Research Institute, Dept of Biomedical Sciences, Cedars-Sinai Medical Center, Suite 400, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA. AD - Department of Radiology, The First Affiliated Hospital of China Medical University, Shenyang, 110001, China. FAU - Tang, Richard AU - Tang R AD - Biomedical Imaging Research Institute, Dept of Biomedical Sciences, Cedars-Sinai Medical Center, Suite 400, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA. FAU - Ho, Hao AU - Ho H AD - Academia Sinica, Taipei, Taiwan. FAU - Jin, Hang AU - Jin H AD - Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. AD - Department of Medical Imaging, Shanghai Medical School, Fudan University and Shanghai Institute of Medical Imaging, Shanghai, 200032, China. FAU - Zeng, Mengsu AU - Zeng M AD - Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai, 200032, China. zengmengsu65@gmail.com. AD - Department of Medical Imaging, Shanghai Medical School, Fudan University and Shanghai Institute of Medical Imaging, Shanghai, 200032, China. zengmengsu65@gmail.com. FAU - Dharmakumar, Rohan AU - Dharmakumar R AUID- ORCID: 0000-0003-4120-4635 AD - Biomedical Imaging Research Institute, Dept of Biomedical Sciences, Cedars-Sinai Medical Center, Suite 400, 8700 Beverly Blvd, Los Angeles, CA, 90048, USA. Rohan.Dharmakumar@cshs.org. AD - Department of Bioengineering, University of California at Los Angeles, Los Angeles, CA, USA. Rohan.Dharmakumar@cshs.org. LA - eng GR - R01 HL133407/HL/NHLBI NIH HHS/United States GR - HL133407/NH/NIH HHS/United States GR - HL136578/NH/NIH HHS/United States PT - Journal Article PT - Research Support, N.I.H., Extramural DEP - 20210930 PL - England TA - J Cardiovasc Magn Reson JT - Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance JID - 9815616 SB - IM EIN - J Cardiovasc Magn Reson. 2022 Feb 7;24(1):11. PMID: 35130921 MH - Animals MH - Dogs MH - Hemorrhage/diagnostic imaging/etiology MH - Humans MH - Magnetic Resonance Imaging MH - *Magnetic Resonance Imaging, Cine MH - Magnetic Resonance Spectroscopy MH - Myocardium MH - Predictive Value of Tests MH - *ST Elevation Myocardial Infarction/diagnostic imaging PMC - PMC8482734 OTO - NOTNLM OT - Hemorrhage OT - Iron OT - Mass spectrometry OT - Myocardial infarction OT - T2* COIS- The authors declare that they have no competing interests. EDAT- 2021/10/01 06:00 MHDA- 2021/11/04 06:00 PMCR- 2021/09/30 CRDT- 2021/09/30 05:40 PHST- 2021/01/30 00:00 [received] PHST- 2021/05/23 00:00 [accepted] PHST- 2021/09/30 05:40 [entrez] PHST- 2021/10/01 06:00 [pubmed] PHST- 2021/11/04 06:00 [medline] PHST- 2021/09/30 00:00 [pmc-release] AID - S1097-6647(23)00445-3 [pii] AID - 779 [pii] AID - 10.1186/s12968-021-00779-4 [doi] PST - epublish SO - J Cardiovasc Magn Reson. 2021 Sep 30;23(1):104. doi: 10.1186/s12968-021-00779-4.